##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062457_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1025503 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15352368544997 32.0 32.0 32.0 32.0 32.0 2 31.370772196668366 32.0 32.0 32.0 32.0 32.0 3 31.473623187840502 32.0 32.0 32.0 32.0 32.0 4 31.59298802636365 32.0 32.0 32.0 32.0 32.0 5 31.51620814371094 32.0 32.0 32.0 32.0 32.0 6 35.1132575916404 36.0 36.0 36.0 36.0 36.0 7 35.126071791111286 36.0 36.0 36.0 36.0 36.0 8 35.069384487417395 36.0 36.0 36.0 36.0 36.0 9 35.199437739333774 36.0 36.0 36.0 36.0 36.0 10 35.07612849499221 36.0 36.0 36.0 36.0 36.0 11 35.2050408433715 36.0 36.0 36.0 36.0 36.0 12 35.109974324794756 36.0 36.0 36.0 36.0 36.0 13 35.150814770897796 36.0 36.0 36.0 36.0 36.0 14 35.10889582965628 36.0 36.0 36.0 36.0 36.0 15 35.067324035132025 36.0 36.0 36.0 36.0 36.0 16 35.06888424509729 36.0 36.0 36.0 36.0 36.0 17 35.045132973769945 36.0 36.0 36.0 36.0 36.0 18 35.061147553932074 36.0 36.0 36.0 36.0 36.0 19 35.02759231323555 36.0 36.0 36.0 36.0 36.0 20 35.011022883404536 36.0 36.0 36.0 36.0 36.0 21 34.97609465793859 36.0 36.0 36.0 36.0 36.0 22 34.961740726258235 36.0 36.0 36.0 36.0 36.0 23 34.88476971788479 36.0 36.0 36.0 32.0 36.0 24 34.87746013419756 36.0 36.0 36.0 32.0 36.0 25 34.842546535700045 36.0 36.0 36.0 32.0 36.0 26 34.77362426048485 36.0 36.0 36.0 32.0 36.0 27 34.74227574175795 36.0 36.0 36.0 32.0 36.0 28 34.70358350975082 36.0 36.0 36.0 32.0 36.0 29 34.671103838799105 36.0 36.0 36.0 32.0 36.0 30 34.65048858950193 36.0 36.0 36.0 32.0 36.0 31 34.63643694850234 36.0 36.0 36.0 32.0 36.0 32 34.5885287512567 36.0 36.0 36.0 32.0 36.0 33 34.54595452182978 36.0 36.0 36.0 32.0 36.0 34 34.51268499458315 36.0 36.0 36.0 32.0 36.0 35 34.44108598414632 36.0 36.0 36.0 32.0 36.0 36 34.40260047995959 36.0 36.0 36.0 32.0 36.0 37 34.3368220278244 36.0 36.0 36.0 32.0 36.0 38 34.276044048627845 36.0 36.0 36.0 32.0 36.0 39 34.16972841620161 36.0 36.0 36.0 32.0 36.0 40 34.0823576332785 36.0 36.0 36.0 32.0 36.0 41 34.085316181425114 36.0 36.0 36.0 32.0 36.0 42 33.92720645380852 36.0 36.0 36.0 32.0 36.0 43 33.96573388863806 36.0 36.0 36.0 32.0 36.0 44 33.94963934771522 36.0 36.0 36.0 32.0 36.0 45 33.76784075716989 36.0 36.0 36.0 32.0 36.0 46 33.88383359190563 36.0 36.0 36.0 32.0 36.0 47 33.768942655457856 36.0 36.0 36.0 32.0 36.0 48 33.77396263102107 36.0 36.0 36.0 32.0 36.0 49 33.80822581698932 36.0 36.0 36.0 32.0 36.0 50 33.217995461739264 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 12.0 21 32.0 22 105.0 23 363.0 24 883.0 25 2315.0 26 5128.0 27 9521.0 28 15741.0 29 22033.0 30 29848.0 31 40312.0 32 59542.0 33 83831.0 34 175157.0 35 580680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86806636472511 17.430980607862185 11.859690802485751 25.841262224926954 2 15.282371939897956 20.99192944407171 36.65900828509463 27.0666903309357 3 18.47824179081773 25.324281471051137 28.634868132361056 27.562608605770073 4 12.066468845044822 16.18132760216206 35.65167532420676 36.100528228586356 5 13.654275024061363 37.399890590276186 33.964503273027965 14.981331112634482 6 34.35648150405414 34.876913100502684 17.255374233906554 13.511231161536625 7 29.413565830621657 30.837647476409135 21.515880499618238 18.23290619335097 8 28.616591077744285 32.63091380522533 20.1145194114498 18.63797570558058 9 27.398457147370607 14.299421844694749 18.319205307054194 39.98291570088045 10 15.26070620953815 27.432684253483412 32.6290610558916 24.67754848108684 11 37.140310657306706 21.187261275686176 21.564734574155317 20.1076934928518 12 23.444885095411717 24.252196239308905 29.55817779177633 22.74474087350305 13 30.452568154359373 19.601795411617516 25.25472865510876 24.690907778914347 14 23.86779950911894 19.417690635717303 25.444976757747174 31.269533097416584 15 25.598657439324896 26.604895353792234 23.982962507179405 23.813484699703462 16 25.881055443036242 25.49724379158325 24.79329655788428 23.828404207496224 17 23.78754620903108 25.678813226289925 26.615816823549025 23.91782374112996 18 25.299682204732704 23.916556070533193 27.743751115306342 23.040010609427764 19 24.536934557968138 27.03775610602797 25.59309919132367 22.83221014468022 20 24.485837681606 23.839910755989987 27.700162749402 23.97408881300201 21 25.78110449213703 25.24829278900208 24.29100646219465 24.67959625666624 22 24.30748617995267 25.448389717046172 25.334104337091162 24.910019765909997 23 23.27170178926829 24.77398895956423 25.594171835674786 26.36013741549269 24 23.419921735967616 26.614159100460945 25.394172420753524 24.571746742817915 25 24.96530970655376 24.5747696496256 24.964919654062445 25.4950009897582 26 23.541520600134763 25.10455844595286 26.070913493183344 25.283007460729028 27 25.4122123484768 25.563552715106635 25.424011436339043 23.600223500077522 28 24.328353988237968 24.105243963206348 26.119962593966083 25.446439454589605 29 23.35907354732263 25.86057768724226 26.92035030614245 23.859998459292658 30 24.98676259357603 24.75331617752459 26.718108089396132 23.541813139503247 31 23.662826924933423 25.36014034088637 25.532543542047172 25.44448919213303 32 23.622944057696564 25.718013501666988 25.627521323682135 25.031521116954313 33 23.386767274205926 25.06818605113783 25.89324458338981 25.651802091266436 34 23.61270517979957 25.89597495082901 26.83239346935114 23.658926400020285 35 25.495781094740828 25.0889563463003 25.95311764080651 23.46214491815236 36 24.079988064393763 25.986077076322545 25.719183659140928 24.214751200142757 37 25.331959048388935 25.608116212239263 25.763259590659416 23.296665148712385 38 24.109339514365146 25.520744454184925 25.258531666899074 25.111384364550858 39 24.0274284911892 25.448682256414656 25.92308359897533 24.60080565342081 40 25.023817580250864 25.44536681023849 25.298804586627245 24.232011022883405 41 23.02382343103823 25.804897694107183 26.491000026328543 24.68027884852604 42 25.684859039905295 25.916647732868654 25.571841330547056 22.826651896678996 43 24.62771927532148 24.240592177692314 25.323475406702855 25.808213140283353 44 24.1136300917696 25.130594449748074 26.56179455350204 24.19398090498029 45 24.662433947048424 25.350194002357867 25.640685595263985 24.346686455329724 46 23.599294393667094 25.23283741312783 26.651948657290436 24.51591953591464 47 24.433570647769923 24.568528809764576 27.057941322453473 23.93995922001203 48 24.289348739106565 27.271202522079406 24.649464701712233 23.789984037101792 49 23.242155313051256 25.64458612017712 27.027614741253803 24.08564382551782 50 23.2768699847782 27.345214982306242 24.406266973377942 24.971648059537614 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13.0 1 13.5 2 14.0 3 77.0 4 140.0 5 160.0 6 180.0 7 195.5 8 211.0 9 287.0 10 363.0 11 539.5 12 716.0 13 1282.5 14 1849.0 15 2533.5 16 3218.0 17 3926.0 18 4634.0 19 5086.0 20 5538.0 21 6212.0 22 6886.0 23 7391.5 24 7897.0 25 8006.5 26 8116.0 27 9503.0 28 10890.0 29 12524.5 30 14159.0 31 15632.5 32 17106.0 33 19485.5 34 21865.0 35 25037.0 36 28209.0 37 32876.5 38 37544.0 39 40678.0 40 43812.0 41 48644.0 42 53476.0 43 56951.0 44 60426.0 45 69441.0 46 78456.0 47 85701.5 48 92947.0 49 89755.0 50 86563.0 51 78695.0 52 70827.0 53 67544.0 54 64261.0 55 64025.5 56 63790.0 57 61552.0 58 59314.0 59 54496.0 60 49678.0 61 43675.5 62 37673.0 63 32557.5 64 27442.0 65 23553.0 66 19664.0 67 17175.5 68 14687.0 69 13450.0 70 12213.0 71 9602.5 72 6992.0 73 6336.5 74 5681.0 75 4042.0 76 2403.0 77 2196.0 78 1989.0 79 1745.5 80 1502.0 81 1171.0 82 840.0 83 753.0 84 666.0 85 539.5 86 413.0 87 286.5 88 160.0 89 102.5 90 45.0 91 30.5 92 16.0 93 13.0 94 10.0 95 6.0 96 2.0 97 3.0 98 4.0 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013554324073162144 2 0.004583116772939719 3 4.875656141425232E-4 4 0.0 5 0.0 6 7.801049826280372E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 1.950262456570093E-4 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1025503.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.60306394396591 #Duplication Level Percentage of deduplicated Percentage of total 1 79.53135788815443 45.812498939817964 2 12.92091896634304 14.885690428661198 3 3.614030878152006 6.245377553089717 4 1.4163998980306858 3.263558955859535 5 0.6873960091503649 1.979805813495772 6 0.40119826005419607 1.3866149416865832 7 0.26256653859623336 1.0587245978613233 8 0.18431231045663868 0.8493563043915082 9 0.12347590790280615 0.6401331556618128 >10 0.6964745965784128 7.48761767417416 >50 0.07575025119074347 3.1022493813405045 >100 0.0777789577216133 9.014532849021915 >500 0.0069779804983869514 2.8302397068669407 >1k 0.0013615571704169661 1.4435996980711387 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 3956 0.3857619139095644 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 3720 0.3627488169220373 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 1471 0.14344180368073034 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 1413 0.13778604255667706 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1106 0.10784951384832614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.751312282850465E-5 2 0.0 0.0 0.0 0.0 9.751312282850465E-5 3 0.0 0.0 0.0 9.751312282850465E-5 9.751312282850465E-5 4 0.0 0.0 0.0 2.9253936848551394E-4 9.751312282850465E-5 5 0.0 9.751312282850465E-5 0.0 3.900524913140186E-4 9.751312282850465E-5 6 0.0 9.751312282850465E-5 0.0 3.900524913140186E-4 2.9253936848551394E-4 7 0.0 9.751312282850465E-5 0.0 4.875656141425232E-4 2.9253936848551394E-4 8 0.0 9.751312282850465E-5 0.0 4.875656141425232E-4 2.9253936848551394E-4 9 0.0 9.751312282850465E-5 0.0 6.825918597995325E-4 2.9253936848551394E-4 10 0.0 9.751312282850465E-5 0.0 6.825918597995325E-4 2.9253936848551394E-4 11 0.0 9.751312282850465E-5 0.0 6.825918597995325E-4 2.9253936848551394E-4 12 0.0 9.751312282850465E-5 0.0 6.825918597995325E-4 4.875656141425232E-4 13 0.0 9.751312282850465E-5 0.0 6.825918597995325E-4 4.875656141425232E-4 14 0.0 9.751312282850465E-5 0.0 8.776181054565419E-4 4.875656141425232E-4 15 0.0 9.751312282850465E-5 0.0 0.0010726443511135512 6.825918597995325E-4 16 0.0 9.751312282850465E-5 0.0 0.001365183719599065 6.825918597995325E-4 17 0.0 9.751312282850465E-5 0.0 0.0014626968424275696 6.825918597995325E-4 18 0.0 9.751312282850465E-5 0.0 0.001657723088084579 7.801049826280372E-4 19 0.0 9.751312282850465E-5 0.0 0.0019502624565700928 7.801049826280372E-4 20 0.0 9.751312282850465E-5 0.0 0.0022428018250556067 8.776181054565419E-4 21 0.0 9.751312282850465E-5 0.0 0.00273036743919813 0.0011701574739420558 22 0.0 9.751312282850465E-5 0.0 0.0037054986674831766 0.0011701574739420558 23 0.0 9.751312282850465E-5 0.0 0.0058507873697102785 0.0011701574739420558 24 0.0 9.751312282850465E-5 0.0 0.008678667931736914 0.0011701574739420558 25 0.0 9.751312282850465E-5 0.0 0.009946338528507475 0.0011701574739420558 26 0.0 9.751312282850465E-5 0.0 0.012774219090534108 0.0011701574739420558 27 0.0 9.751312282850465E-5 0.0 0.016479717758017286 0.0011701574739420558 28 0.0 9.751312282850465E-5 0.0 0.0231106101103556 0.0011701574739420558 29 0.0 9.751312282850465E-5 0.0 0.03715249979766027 0.0011701574739420558 30 0.0 9.751312282850465E-5 0.0 0.05519242752093363 0.0011701574739420558 31 0.0 9.751312282850465E-5 0.0 0.09410016352950698 0.0012676705967705603 32 0.0 9.751312282850465E-5 0.0 0.14305175118941632 0.0012676705967705603 33 0.0 9.751312282850465E-5 0.0 0.19141826011235463 0.0012676705967705603 34 0.0 9.751312282850465E-5 0.0 0.250998778160571 0.0012676705967705603 35 0.0 9.751312282850465E-5 0.0 0.3117494536827293 0.0012676705967705603 36 0.0 9.751312282850465E-5 0.0 0.4049719991067798 0.0012676705967705603 37 0.0 9.751312282850465E-5 0.0 0.5447083041200269 0.0012676705967705603 38 0.0 9.751312282850465E-5 0.0 0.7101880735599994 0.0012676705967705603 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTATA 75 6.184564E-11 32.266346 44 TAGGACG 95 9.622454E-10 25.473429 4 GTATTAC 110 5.2368705E-9 22.005146 1 TTAACGG 95 6.864866E-7 20.841896 35 GGACCGT 80 8.9875684E-5 19.249807 6 TGGTATA 325 0.0 18.953657 44 GTATTAT 105 1.7881412E-6 18.861553 1 TAACGGC 105 1.7923176E-6 18.856955 36 TGCGCCT 60 0.007411781 18.33315 10 TACACCG 60 0.007411781 18.33315 5 GTCTTAG 160 1.0968506E-9 17.87918 1 TCTATAC 150 9.586074E-9 17.600683 3 TGCGTCC 75 0.0012907754 17.599825 26 CTCGTAT 90 2.2151273E-4 17.11094 43 CGAGCCG 350 0.0 16.971258 15 GTCGTAT 95 3.3459498E-4 16.210363 43 GATATAA 110 5.60868E-5 16.003742 1 GGCGTTA 275 0.0 15.99984 42 ATTACAC 195 8.913048E-10 15.795484 3 GTATTAG 280 0.0 15.71796 1 >>END_MODULE