FastQCFastQC Report
Thu 2 Feb 2017
SRR4062456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817252
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37440.20602536136980454No Hit
TATCAACGCAGAGTACTTTTTTTTT25210.1387259444479907No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23070.1269499221902081No Hit
GATTAAGAGGGACGGCCGGGGGCAT21880.12040157336461867No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20580.11324791498372269No Hit
GAATAGGACCGCGGTTCTATTTTGT20270.11154204260058595No Hit
GAATAACGCCGCCGCATCGCCAGTC20210.1112118737522369No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19710.1084604666826615No Hit
GTATCTGATCGTCTTCGAACCTCCG19330.10636939730978422No Hit
GCGCAAGACGGACCAGAGCGAAAGC19230.10581911589586915No Hit
GTCCTATTCCATTATTCCTAGCTGC18430.10141686458454853No Hit
CTATTGGAGCTGGAATTACCGCGGC18200.10015121733254387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT1101.8553692E-1013.8192847
AATCGTA1001.0068106E-813.300693513
GCGTTAT1800.012.65860751
AAGACGG3350.012.4768895
TAATACT2450.012.4064184
GGCGATC550.003066320412.0922088
ATAACGA1603.6379788E-1211.87594712
CAATTAG1052.7431997E-711.7544211
ATCGCCA3000.011.7185716
GTATTAT1302.6411726E-911.6848691
CGCATCG3100.011.64576913
TTTACGA907.4511827E-611.61267810
TACCGGC1157.0675014E-811.5661417
CAAGACG3550.011.50542454
GCGCGCT1001.9274848E-611.40122315
CGTTATT2100.011.3026392
CGAACGA1701.0913936E-1111.17828716
CGATAAC1701.0913936E-1111.17797810
CGTTCAA855.3194446E-511.17736214
ATCCCCG3250.011.10857912
Produced by FastQC (version 0.11.5)