##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062456_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1817252 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.166468381930517 32.0 32.0 32.0 32.0 32.0 2 30.846831369562395 32.0 32.0 32.0 32.0 32.0 3 30.83716347540132 32.0 32.0 32.0 32.0 32.0 4 30.896887168097766 32.0 32.0 32.0 32.0 32.0 5 30.720952295003666 32.0 32.0 32.0 32.0 32.0 6 34.3601663390658 36.0 36.0 36.0 32.0 36.0 7 34.230269659904074 36.0 36.0 36.0 32.0 36.0 8 34.23982859834519 36.0 36.0 36.0 32.0 36.0 9 34.37633470756945 36.0 36.0 36.0 32.0 36.0 10 34.07314835807032 36.0 36.0 36.0 32.0 36.0 11 34.35295985366917 36.0 36.0 36.0 32.0 36.0 12 34.15163444585561 36.0 36.0 36.0 32.0 36.0 13 34.272853737401306 36.0 36.0 36.0 32.0 36.0 14 34.17938843924783 36.0 36.0 36.0 32.0 36.0 15 34.1250987755138 36.0 36.0 36.0 32.0 36.0 16 34.127993255750994 36.0 36.0 36.0 32.0 36.0 17 34.055200379474066 36.0 36.0 36.0 32.0 36.0 18 34.06534591790241 36.0 36.0 36.0 32.0 36.0 19 34.04995179534814 36.0 36.0 36.0 32.0 36.0 20 34.03581891779456 36.0 36.0 36.0 32.0 36.0 21 34.0186022631974 36.0 36.0 36.0 32.0 36.0 22 33.96578570280842 36.0 36.0 36.0 32.0 36.0 23 33.9455771681638 36.0 36.0 36.0 32.0 36.0 24 33.909424779832406 36.0 36.0 36.0 32.0 36.0 25 33.46494157111947 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 67.0 5 180.0 6 306.0 7 63.0 8 270.0 9 365.0 10 206.0 11 66.0 12 134.0 13 97.0 14 314.0 15 420.0 16 647.0 17 920.0 18 1274.0 19 1758.0 20 2641.0 21 3786.0 22 5559.0 23 8061.0 24 11202.0 25 15680.0 26 21481.0 27 27677.0 28 37368.0 29 49335.0 30 63317.0 31 85515.0 32 122741.0 33 178335.0 34 404699.0 35 772759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.128240405702506 17.58323037595723 11.136181652582831 26.152347565757434 2 17.284596708335258 20.169205743354045 36.336311548121394 26.209886000189304 3 18.98172096739755 23.984626593967302 28.226181016132827 28.807471422502317 4 12.894913537848518 15.486961881803873 35.57144272364033 36.04668185670729 5 15.085271326363953 36.15927002614205 33.05882981130268 15.696628836191307 6 33.944033860681444 35.24475000880871 16.973558454599907 13.83765767590994 7 30.193234054831514 30.441852474290158 20.811151320788795 18.553762150089536 8 28.445908992522252 31.828713966820754 19.705802815846422 20.019574224810576 9 27.83881916315162 14.129569061528175 18.67541417748906 39.35619759783115 10 16.53746285998884 26.14469467371057 30.761983834419322 26.555858631881268 11 37.49914604503152 21.120565219020165 22.066031103794646 19.314257632153662 12 24.906991710801524 23.82836003464669 28.152914884191453 23.11173337036033 13 29.536434057276345 19.262503718885327 25.17751479289941 26.02354743093892 14 23.84422124396009 19.772673428796537 24.64586971972606 31.737235607517313 15 25.6438014143137 26.737371333373 22.037830357280587 25.580996895032715 16 26.159792465584005 25.365176725427318 23.403042170715675 25.071988638273 17 24.340438660767905 25.355110271229208 24.95887210961561 25.345578958387282 18 25.162240165033705 24.40864575607939 25.9488391108721 24.4802749680148 19 25.958106367245914 24.65873814385729 25.090777251633988 24.292378237262806 20 26.067060152931838 23.964867219046013 24.622091757999886 25.34598087002226 21 27.29712098137443 23.769314858363217 23.90127630688668 25.032287853375678 22 26.275312256136 24.11305149084945 24.392902648390553 25.218733604623996 23 24.66360102163677 23.942118270711703 25.188856775231645 26.205423932419887 24 24.971099030315987 24.92189299422862 24.703689295565727 25.403318679889665 25 24.99156976487909 24.323101709409244 24.988649487353545 25.696679038358123 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 279.0 1 279.0 2 653.0 3 1027.0 4 1027.0 5 1027.0 6 2617.5 7 4208.0 8 4208.0 9 4208.0 10 4357.5 11 4507.0 12 4507.0 13 4507.0 14 5039.5 15 5572.0 16 5572.0 17 5572.0 18 8939.5 19 12307.0 20 12307.0 21 12307.0 22 19582.0 23 26857.0 24 26857.0 25 26857.0 26 40187.5 27 53518.0 28 53518.0 29 53518.0 30 65142.0 31 76766.0 32 76766.0 33 76766.0 34 95157.0 35 113548.0 36 113548.0 37 113548.0 38 127999.0 39 142450.0 40 142450.0 41 142450.0 42 161653.5 43 180857.0 44 180857.0 45 180857.0 46 205254.5 47 229652.0 48 229652.0 49 229652.0 50 232306.5 51 234961.0 52 234961.0 53 234961.0 54 217510.5 55 200060.0 56 200060.0 57 200060.0 58 185489.5 59 170919.0 60 170919.0 61 170919.0 62 154355.5 63 137792.0 64 137792.0 65 137792.0 66 117694.0 67 97596.0 68 97596.0 69 97596.0 70 75654.0 71 53712.0 72 53712.0 73 53712.0 74 43332.0 75 32952.0 76 32952.0 77 32952.0 78 27005.0 79 21058.0 80 21058.0 81 21058.0 82 15227.0 83 9396.0 84 9396.0 85 9396.0 86 7005.5 87 4615.0 88 4615.0 89 4615.0 90 3098.5 91 1582.0 92 1582.0 93 1582.0 94 937.0 95 292.0 96 292.0 97 292.0 98 530.5 99 769.0 100 769.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008639418198466696 2 0.0030815759179244266 3 0.004842476442452671 4 0.014967654458490073 5 0.035162982349173366 6 0.047764426727828616 7 0.06691421993207326 8 0.08798999808502068 9 0.09497857204174215 10 0.11891581354704794 11 0.11930101053678852 12 0.129866413683958 13 0.12062168593018469 14 0.12337309299976008 15 0.11533898435659998 16 0.11198226773171799 17 0.12007140451626962 18 0.1293161322700429 19 0.13041669509787304 20 0.12078677035435921 21 0.12810551315942972 22 0.1494564320193347 23 0.13850583188242468 24 0.13393849614692954 25 0.12964630111839195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1817252.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.092000293613 #Duplication Level Percentage of deduplicated Percentage of total 1 79.42711931364957 38.992361646665195 2 12.471761973016596 12.245274848824044 3 3.4113729339670664 5.024133632278039 4 1.4152936398302407 2.7791838312837918 5 0.7691702871068115 1.8880053980242992 6 0.45723378393946407 1.3467912633243573 7 0.32946737945105187 1.132194888212286 8 0.23316634115161622 0.9157281670620651 9 0.18604351609721206 0.8219923512182217 >10 1.0442072455671758 9.58570601810343 >50 0.12010867380920891 4.168668794293468 >100 0.11548870252408618 12.280119200181481 >500 0.014281084229965515 4.83910114665576 >1k 0.005285125659908498 3.9807388138736113 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3744 0.20602536136980454 No Hit TATCAACGCAGAGTACTTTTTTTTT 2521 0.1387259444479907 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2307 0.1269499221902081 No Hit GATTAAGAGGGACGGCCGGGGGCAT 2188 0.12040157336461867 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 2058 0.11324791498372269 No Hit GAATAGGACCGCGGTTCTATTTTGT 2027 0.11154204260058595 No Hit GAATAACGCCGCCGCATCGCCAGTC 2021 0.1112118737522369 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 1971 0.1084604666826615 No Hit GTATCTGATCGTCTTCGAACCTCCG 1933 0.10636939730978422 No Hit GCGCAAGACGGACCAGAGCGAAAGC 1923 0.10581911589586915 No Hit GTCCTATTCCATTATTCCTAGCTGC 1843 0.10141686458454853 No Hit CTATTGGAGCTGGAATTACCGCGGC 1820 0.10015121733254387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.5028141391507615E-5 2 0.0 0.0 0.0 0.0 5.5028141391507615E-5 3 0.0 0.0 0.0 0.0 5.5028141391507615E-5 4 0.0 0.0 0.0 0.0 5.5028141391507615E-5 5 0.0 0.0 0.0 0.0 5.5028141391507615E-5 6 0.0 0.0 0.0 0.0 5.5028141391507615E-5 7 0.0 0.0 0.0 5.5028141391507615E-5 5.5028141391507615E-5 8 0.0 0.0 0.0 5.5028141391507615E-5 5.5028141391507615E-5 9 0.0 0.0 0.0 1.1005628278301523E-4 5.5028141391507615E-5 10 0.0 0.0 0.0 1.1005628278301523E-4 5.5028141391507615E-5 11 0.0 0.0 0.0 1.1005628278301523E-4 5.5028141391507615E-5 12 0.0 0.0 0.0 1.1005628278301523E-4 2.2011256556603046E-4 13 0.0 0.0 0.0 1.1005628278301523E-4 2.2011256556603046E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGTT 110 1.8553692E-10 13.819284 7 AATCGTA 100 1.0068106E-8 13.3006935 13 GCGTTAT 180 0.0 12.6586075 1 AAGACGG 335 0.0 12.476889 5 TAATACT 245 0.0 12.406418 4 GGCGATC 55 0.0030663204 12.092208 8 ATAACGA 160 3.6379788E-12 11.875947 12 CAATTAG 105 2.7431997E-7 11.754421 1 ATCGCCA 300 0.0 11.71857 16 GTATTAT 130 2.6411726E-9 11.684869 1 CGCATCG 310 0.0 11.645769 13 TTTACGA 90 7.4511827E-6 11.612678 10 TACCGGC 115 7.0675014E-8 11.566141 7 CAAGACG 355 0.0 11.5054245 4 GCGCGCT 100 1.9274848E-6 11.401223 15 CGTTATT 210 0.0 11.302639 2 CGAACGA 170 1.0913936E-11 11.178287 16 CGATAAC 170 1.0913936E-11 11.177978 10 CGTTCAA 85 5.3194446E-5 11.177362 14 ATCCCCG 325 0.0 11.108579 12 >>END_MODULE