FastQCFastQC Report
Thu 2 Feb 2017
SRR4062456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817252
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22590.1243085714034157No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20300.11170712702476046No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19460.10708476314787382No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG19430.1069196787236993No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19180.1055439751889116No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18710.10295765254351075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2450.018.8570484
CGGATCG1203.164314E-718.3332426
TGGTCGG7300.018.08210237
GTCGGAA7450.018.01333439
GTATCAA42350.017.7695671
CGTTTAT7400.017.54045331
ATCGTTT7700.016.57134429
GGTCGGA8000.016.49991838
TACATCG800.001988935516.4999185
CGAATTA800.001988935516.49991841
CATCGTT7900.016.43029828
TCGATTT1351.0862386E-616.29621330
CGCAATA7200.016.19436536
ATTTCGT2051.05501385E-1016.0974842
GTTCAAA8650.016.0261291
TATGGTC8550.015.953135535
ATTACAC2353.6379788E-1215.9148133
ATAATAC1259.445865E-615.839923
ATGGTCG8200.015.82918936
GCATCGT8200.015.82918927
Produced by FastQC (version 0.11.5)