##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1817252
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28080695467662	32.0	32.0	32.0	32.0	32.0
2	31.40676582004037	32.0	32.0	32.0	32.0	32.0
3	31.481859422908876	32.0	32.0	32.0	32.0	32.0
4	31.588607964112846	32.0	32.0	32.0	32.0	32.0
5	31.521042761268113	32.0	32.0	32.0	32.0	32.0
6	35.102880613145565	36.0	36.0	36.0	36.0	36.0
7	35.1192146163548	36.0	36.0	36.0	36.0	36.0
8	35.05865614675345	36.0	36.0	36.0	36.0	36.0
9	35.193269838195256	36.0	36.0	36.0	36.0	36.0
10	35.05737839331034	36.0	36.0	36.0	36.0	36.0
11	35.19623310360919	36.0	36.0	36.0	36.0	36.0
12	35.09659474855441	36.0	36.0	36.0	36.0	36.0
13	35.144636517114854	36.0	36.0	36.0	36.0	36.0
14	35.106297860725974	36.0	36.0	36.0	36.0	36.0
15	35.06998203881465	36.0	36.0	36.0	36.0	36.0
16	35.081335444946546	36.0	36.0	36.0	36.0	36.0
17	35.05225032081407	36.0	36.0	36.0	36.0	36.0
18	35.067524482020104	36.0	36.0	36.0	36.0	36.0
19	35.04602443689703	36.0	36.0	36.0	36.0	36.0
20	35.03847952843084	36.0	36.0	36.0	36.0	36.0
21	35.03970170345114	36.0	36.0	36.0	36.0	36.0
22	35.018121317241636	36.0	36.0	36.0	36.0	36.0
23	34.98501803822475	36.0	36.0	36.0	36.0	36.0
24	34.950591882688805	36.0	36.0	36.0	32.0	36.0
25	34.92770402783984	36.0	36.0	36.0	32.0	36.0
26	34.88885526058026	36.0	36.0	36.0	32.0	36.0
27	34.872131658129966	36.0	36.0	36.0	32.0	36.0
28	34.8503036452842	36.0	36.0	36.0	32.0	36.0
29	34.83182354456069	36.0	36.0	36.0	32.0	36.0
30	34.821215219463234	36.0	36.0	36.0	32.0	36.0
31	34.819267223257974	36.0	36.0	36.0	32.0	36.0
32	34.77938667834731	36.0	36.0	36.0	32.0	36.0
33	34.7584627778646	36.0	36.0	36.0	32.0	36.0
34	34.739190822186465	36.0	36.0	36.0	32.0	36.0
35	34.71528439644034	36.0	36.0	36.0	32.0	36.0
36	34.68739613438312	36.0	36.0	36.0	32.0	36.0
37	34.68192303544033	36.0	36.0	36.0	32.0	36.0
38	34.63977973335564	36.0	36.0	36.0	32.0	36.0
39	34.64332024397277	36.0	36.0	36.0	32.0	36.0
40	34.63161575829879	36.0	36.0	36.0	32.0	36.0
41	34.59442774034641	36.0	36.0	36.0	32.0	36.0
42	34.56727410397677	36.0	36.0	36.0	32.0	36.0
43	34.56415455864129	36.0	36.0	36.0	32.0	36.0
44	34.525649166983996	36.0	36.0	36.0	32.0	36.0
45	34.48095916251571	36.0	36.0	36.0	32.0	36.0
46	34.48815519256548	36.0	36.0	36.0	32.0	36.0
47	34.45719691050003	36.0	36.0	36.0	32.0	36.0
48	34.407515853607535	36.0	36.0	36.0	32.0	36.0
49	34.39810934311807	36.0	36.0	36.0	32.0	36.0
50	33.86486023952649	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	9.0
21	45.0
22	150.0
23	452.0
24	1291.0
25	2953.0
26	6152.0
27	11099.0
28	18831.0
29	28756.0
30	41235.0
31	58687.0
32	86945.0
33	143273.0
34	317166.0
35	1100203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.507219337422505	18.206929573278956	11.67097322777443	26.61487786152411
2	16.693750711953612	20.657193028990477	35.99010116207339	26.658955096982528
3	18.38193618255316	24.2600066254101	28.616874105101896	28.741183086934846
4	12.653742002334297	15.910587927136016	35.711231647396694	35.72443842313299
5	14.747164811025005	36.39612799772844	33.04342658223878	15.813280609007782
6	34.12533808044595	35.474908446577096	16.6737011245044	13.72605234847255
7	30.185593514599802	30.515267291089675	20.808297808062836	18.490841386247688
8	28.498469117106488	31.9695892479414	19.56255929282235	19.969382342129766
9	27.915390930922072	13.92729241734223	18.600887493864363	39.556429157871335
10	16.530728814715843	26.25464162372637	30.713902089528585	26.500727472029194
11	37.79800489970571	20.923900482706856	22.037890177036537	19.240204440550897
12	24.92587709354564	23.770891433879285	28.145738730786924	23.15749274178815
13	29.75971411779984	19.091463374369653	25.165634705588435	25.98318780224207
14	23.991939477848973	19.606251637087208	24.519824438217704	31.88198444684612
15	25.783545705273674	26.67522170838167	21.94489261808489	25.59633996825977
16	26.330250289998304	25.341449617334305	23.32235705339711	25.005943039270285
17	24.571220722277374	25.30914809833749	24.857972367068516	25.261658812316618
18	25.455963179570034	24.26036675155675	25.869884859116954	24.41378520975627
19	26.065564929905154	24.599725299518173	25.069170373729126	24.26553939684755
20	26.18112402682732	23.80055160208931	24.60313704428445	25.41518732679893
21	27.494425649277037	23.72510802024155	23.90895704063058	24.87150928985083
22	26.334322372461276	23.970808671554632	24.38753678631252	25.30733216967157
23	24.86721709482229	23.702601510412425	25.1982663934336	26.23191500133168
24	25.10400318722995	24.74054231333904	24.71897128191357	25.436483217517434
25	25.26000796807487	24.046362309685172	25.024982776191745	25.668646946048206
26	24.895501559497525	24.82539570736475	25.39449674563572	24.88460598750201
27	25.878304164749856	24.36161853171712	24.39056333408905	25.369513969443975
28	24.82071831534647	24.358096730668063	25.462566556537013	25.358618397448456
29	24.91377090243951	24.512945920543768	25.093272699658602	25.48001047735812
30	24.90414097769599	24.677094866314633	25.434557232568732	24.984206923420636
31	25.4769289014402	24.438492845241054	24.318916693997313	25.765661559321433
32	25.18682054002417	24.42115898070273	24.458027835435043	25.93399264383806
33	24.930499457422524	23.954190172854396	25.022121312839385	26.09318905688369
34	25.38283077966072	24.273518477349317	25.318392826091262	25.025257916898703
35	26.258493593623776	23.949787921543077	25.00808913678455	24.783629348048592
36	24.91465135270177	24.684083440271355	24.75022726622395	25.65103794080293
37	26.068151252550553	24.476462262801196	24.35908723721311	25.096299247435137
38	25.01419726047901	24.173312231875382	25.15149247325082	25.660998034394787
39	25.479845392933946	24.140185290757692	24.494291380612047	25.885677935696314
40	25.782830339435588	24.3021331108729	25.094373262486435	24.82066328720508
41	24.57193608811546	24.709011188321707	25.521281583401752	25.19777114016108
42	25.956554181808578	24.770642706680196	24.94744812497111	24.325354986540116
43	24.99367176373998	23.768635280082233	25.238822133639143	25.998870822538645
44	24.730692276029963	24.33245361677962	24.93022431671557	26.00662979047485
45	24.836786532632786	24.613317250441877	25.056403844926294	25.49349237199904
46	24.619659034442684	24.238754030125722	25.217595147835937	25.923991787595657
47	24.91789801304387	24.091265273060642	25.59633996825977	25.39449674563572
48	25.47555319790541	24.84184912164081	24.639950870875367	25.042646809578418
49	24.449388417236577	24.781538278675715	25.14455892743549	25.62451437665222
50	24.313495666529096	25.18602283670381	24.830100426468565	25.670381070298525
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	17.0
2	20.0
3	86.5
4	153.0
5	184.0
6	215.0
7	218.0
8	221.0
9	315.0
10	409.0
11	581.5
12	754.0
13	1403.5
14	2053.0
15	2956.0
16	3859.0
17	4689.5
18	5520.0
19	5908.5
20	6297.0
21	7109.5
22	7922.0
23	8515.0
24	9108.0
25	10717.5
26	12327.0
27	15099.0
28	17871.0
29	20709.5
30	23548.0
31	25387.5
32	27227.0
33	31519.0
34	35811.0
35	41896.5
36	47982.0
37	57533.5
38	67085.0
39	72629.0
40	78173.0
41	85587.5
42	93002.0
43	93918.0
44	94834.0
45	104087.0
46	113340.0
47	119954.0
48	126568.0
49	130233.0
50	133898.0
51	128838.0
52	123778.0
53	121402.0
54	119026.0
55	121865.5
56	124705.0
57	123048.0
58	121391.0
59	111211.0
60	101031.0
61	89555.5
62	78080.0
63	69970.5
64	61861.0
65	53582.0
66	45303.0
67	41718.0
68	38133.0
69	35316.5
70	32500.0
71	26346.0
72	20192.0
73	17924.5
74	15657.0
75	11970.0
76	8283.0
77	7490.0
78	6697.0
79	5805.0
80	4913.0
81	3862.0
82	2811.0
83	2508.0
84	2205.0
85	1828.5
86	1452.0
87	1078.0
88	704.0
89	458.0
90	212.0
91	132.5
92	53.0
93	39.0
94	25.0
95	20.0
96	15.0
97	13.5
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014142232337617456
2	0.0037969417560140255
3	3.301688483490457E-4
4	1.6508442417452284E-4
5	5.5028141391507615E-5
6	3.851969897405533E-4
7	5.5028141391507615E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	5.5028141391507615E-5
47	0.0
48	0.0
49	0.0
50	1.1005628278301523E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1817252.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.562172997751055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.09716659959335	42.10081127437513
2	12.285913639109838	12.91548636268641
3	3.2879514092808293	5.18465612348455
4	1.356302504759139	2.8516082756973176
5	0.7157489303557744	1.8810659550157742
6	0.42446733517498175	1.3386555302017065
7	0.2891646673918089	1.0639386290599784
8	0.22845719829323882	0.9606565423416589
9	0.16001973567876482	0.7569886526821469
>10	0.9415353906541163	9.226447931461227
>50	0.0994778206448232	3.672653314568601
>100	0.09800518183966263	10.908317710554583
>500	0.012105350204831429	4.342385986607086
>1k	0.0036842370188617394	2.796327711263868
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2259	0.1243085714034157	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2030	0.11170712702476046	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1946	0.10708476314787382	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1943	0.1069196787236993	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1918	0.1055439751889116	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1871	0.10295765254351075	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5028141391507615E-5
2	0.0	0.0	0.0	0.0	5.5028141391507615E-5
3	0.0	0.0	0.0	0.0	5.5028141391507615E-5
4	0.0	0.0	0.0	0.0	5.5028141391507615E-5
5	0.0	0.0	0.0	0.0	5.5028141391507615E-5
6	0.0	0.0	0.0	0.0	5.5028141391507615E-5
7	0.0	0.0	0.0	0.0	5.5028141391507615E-5
8	0.0	0.0	0.0	0.0	5.5028141391507615E-5
9	0.0	0.0	0.0	0.0	5.5028141391507615E-5
10	0.0	0.0	0.0	0.0	5.5028141391507615E-5
11	0.0	0.0	0.0	0.0	5.5028141391507615E-5
12	0.0	0.0	0.0	0.0	1.1005628278301523E-4
13	0.0	0.0	0.0	0.0	1.1005628278301523E-4
14	0.0	0.0	0.0	1.1005628278301523E-4	1.1005628278301523E-4
15	0.0	0.0	0.0	1.1005628278301523E-4	1.6508442417452284E-4
16	0.0	0.0	0.0	1.1005628278301523E-4	1.6508442417452284E-4
17	0.0	0.0	0.0	2.2011256556603046E-4	1.6508442417452284E-4
18	0.0	0.0	0.0	3.851969897405533E-4	1.6508442417452284E-4
19	0.0	0.0	0.0	3.851969897405533E-4	1.6508442417452284E-4
20	0.0	0.0	0.0	6.053095553065838E-4	1.6508442417452284E-4
21	0.0	0.0	0.0	6.603376966980914E-4	1.6508442417452284E-4
22	0.0	0.0	0.0	9.354784036556295E-4	1.6508442417452284E-4
23	0.0	0.0	0.0	0.001870956807311259	2.2011256556603046E-4
24	0.0	0.0	0.0	0.0030815759179244266	2.2011256556603046E-4
25	0.0	0.0	0.0	0.003741913614622518	2.2011256556603046E-4
26	0.0	0.0	0.0	0.00462236387688664	2.751407069575381E-4
27	0.0	0.0	0.0	0.006053095553065838	3.301688483490457E-4
28	0.0	0.0	0.0	0.013041669509787305	3.301688483490457E-4
29	0.0	0.0	0.0	0.02657859229209818	3.301688483490457E-4
30	0.0	0.0	0.0	0.055303282098465155	3.301688483490457E-4
31	0.0	0.0	0.0	0.11660463160860464	3.851969897405533E-4
32	0.0	0.0	0.0	0.19127781947688047	3.851969897405533E-4
33	0.0	0.0	0.0	0.26127361532687815	3.851969897405533E-4
34	0.0	0.0	0.0	0.3472826003218046	3.851969897405533E-4
35	0.0	0.0	0.0	0.4429765382016363	3.851969897405533E-4
36	0.0	0.0	0.0	0.578125653459179	3.851969897405533E-4
37	0.0	0.0	0.0	0.7708892327536302	3.851969897405533E-4
38	0.0	0.0	0.0	1.0230281766095182	3.851969897405533E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	245	0.0	18.857048	4
CGGATCG	120	3.164314E-7	18.33324	26
TGGTCGG	730	0.0	18.082102	37
GTCGGAA	745	0.0	18.013334	39
GTATCAA	4235	0.0	17.769567	1
CGTTTAT	740	0.0	17.540453	31
ATCGTTT	770	0.0	16.571344	29
GGTCGGA	800	0.0	16.499918	38
TACATCG	80	0.0019889355	16.499918	5
CGAATTA	80	0.0019889355	16.499918	41
CATCGTT	790	0.0	16.430298	28
TCGATTT	135	1.0862386E-6	16.296213	30
CGCAATA	720	0.0	16.194365	36
ATTTCGT	205	1.05501385E-10	16.09748	42
GTTCAAA	865	0.0	16.026129	1
TATGGTC	855	0.0	15.9531355	35
ATTACAC	235	3.6379788E-12	15.914813	3
ATAATAC	125	9.445865E-6	15.83992	3
ATGGTCG	820	0.0	15.829189	36
GCATCGT	820	0.0	15.829189	27
>>END_MODULE