FastQCFastQC Report
Thu 2 Feb 2017
SRR4062455_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062455_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992444
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG145261.4636594105057819No Hit
TCCATGTACTCTGCGTTGATACCAC121951.2287846971718304No Hit
GAGTACATGGAAGCAGTGGTATCAA58270.5871364026584875No Hit
CATGTACTCTGCGTTGATACCACTG48470.48839027693250203No Hit
GTATCAACGCAGAGTACTTTTTTTT43490.4382111232472563No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36880.3716078690586068No Hit
GCTTCCATGTACTCTGCGTTGATAC35450.35719899561083546No Hit
TATCAACGCAGAGTACTTTTTTTTT32590.32838124871529273No Hit
CATGGAAGCAGTGGTATCAACGCAG31590.31830511343713097No Hit
GCGTTGATACCACTGCTTCCATGTA31260.31497998879533756No Hit
CCCATGTACTCTGCGTTGATACCAC30420.3065160351616817No Hit
GTACATGGGAAGCAGTGGTATCAAC28010.28223254914131174No Hit
GTATCAACGCAGAGTACATGGAAGC25510.25704221094590723No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24560.24746988243165355No Hit
ACTCTGCGTTGATACCACTGCTTCC21000.2115988408413976No Hit
ACGCAGAGTACATGGAAGCAGTGGT20220.2037394553244314No Hit
CAGTGGTATCAACGCAGAGTACATG18750.1889275364655336No Hit
TATCAACGCAGAGTACATGGAAGCA17660.17794454901233722No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17250.17381333354829087No Hit
GGTATCAACGCAGAGTACTTTTTTT15070.15184735864189816No Hit
GTACATGGGGTGGTATCAACGCAAA14170.14277883689155257No Hit
GTATCAACGCAGAGTACATGGGAAG13960.1406628484831386No Hit
ATACCACTGCTTCCATGTACTCTGC11520.11607707840442381No Hit
GGTATCAACGCAGAGTACATGGAAG11210.11295347646819366No Hit
AAGCAGTGGTATCAACGCAGAGTAC10040.10116439819274439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA1055.456968E-1215.3727861
TGGGTTA456.773577E-414.7736575
GTTTAAG653.3877623E-614.6076261
TAGTACT759.692958E-713.9294484
CGAGCCG2000.013.77741515
TTAAGAC707.2809216E-613.566963
CGTTATT802.0069892E-613.0568812
CTCGTAG604.0923568E-412.66569110
CGTCGTA905.403381E-712.66569110
AACTCGG604.0923568E-412.66569110
GCTCGTA604.0923568E-412.6656919
GTATAGT604.1080633E-412.6599421
AGGCCCG1900.012.49903810
CGCCAGT1001.4314173E-712.35590718
GAGCCGC2400.012.27546416
CGACCCG701.09297325E-412.210885
GCGTTAT701.09555E-412.20781
CTATTCC1950.012.1760914
CCTATTC1800.012.13489153
GTAGTAC550.00307467112.0869283
Produced by FastQC (version 0.11.5)