##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062455_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 992444 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.005412899871427 32.0 32.0 32.0 32.0 32.0 2 30.69458528642422 32.0 32.0 32.0 32.0 32.0 3 30.70069444724337 32.0 32.0 32.0 32.0 32.0 4 30.73743203646755 32.0 32.0 32.0 32.0 32.0 5 30.56677857894249 32.0 32.0 32.0 32.0 32.0 6 34.16641745025412 36.0 36.0 36.0 32.0 36.0 7 33.99952239118782 36.0 36.0 36.0 32.0 36.0 8 33.9843658685024 36.0 36.0 36.0 32.0 36.0 9 34.14194151004994 36.0 36.0 36.0 32.0 36.0 10 33.84803072012124 36.0 36.0 36.0 32.0 36.0 11 34.17944891600936 36.0 36.0 36.0 32.0 36.0 12 33.96595576173568 36.0 36.0 36.0 32.0 36.0 13 34.06244684838641 36.0 36.0 36.0 32.0 36.0 14 33.98085232013091 36.0 36.0 36.0 32.0 36.0 15 33.93000209583614 36.0 36.0 36.0 32.0 36.0 16 33.90730157066797 36.0 36.0 36.0 32.0 36.0 17 33.82486870795733 36.0 36.0 36.0 32.0 36.0 18 33.851407233052946 36.0 36.0 36.0 32.0 36.0 19 33.834386625341075 36.0 36.0 36.0 32.0 36.0 20 33.80509933054157 36.0 36.0 36.0 32.0 36.0 21 33.72835646142251 36.0 36.0 36.0 32.0 36.0 22 33.680699364397384 36.0 36.0 36.0 27.0 36.0 23 33.60825598220151 36.0 36.0 36.0 27.0 36.0 24 33.58758579829189 36.0 36.0 36.0 27.0 36.0 25 33.072991523955004 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 35.0 5 86.0 6 171.0 7 47.0 8 144.0 9 170.0 10 128.0 11 42.0 12 66.0 13 50.0 14 255.0 15 351.0 16 474.0 17 700.0 18 926.0 19 1252.0 20 1964.0 21 2727.0 22 3976.0 23 5411.0 24 7487.0 25 10125.0 26 13597.0 27 17206.0 28 22551.0 29 29790.0 30 38525.0 31 51837.0 32 74248.0 33 104764.0 34 222621.0 35 380716.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.457067424448546 16.922216511986456 11.991394338805081 24.62932172475992 2 15.061113854153021 21.104079966949143 37.339909916264446 26.49489626263339 3 19.21499553608316 26.017178522413296 28.084082861582303 26.68374307992124 4 12.44356659142212 16.21745001612383 35.05572799097065 36.283255401483395 5 13.570631571202071 37.58563792632493 33.78435722543702 15.059373277035984 6 33.37372267041393 34.31917589623502 18.123186134024724 14.18391529932632 7 28.703453504926372 31.17299385765736 21.894446886683255 18.22910575073301 8 28.87379764783713 32.05744666689526 20.628520832030723 18.4402348532369 9 27.264585297959144 15.264423925486263 18.544117721218967 38.92687305533563 10 15.720272045160627 27.71717310295711 32.08393775484058 24.47861709704168 11 36.301357425894395 21.905238437265456 21.38137453494847 20.41202960189168 12 23.448686387666974 24.461943581258325 29.52732152225675 22.56204850881795 13 30.51658253336077 20.235674135767347 25.218100968037415 24.029642362834466 14 24.355845735885907 19.493554169890675 25.250766447988976 30.89983364623444 15 25.61512948183058 26.218154087009854 25.085944624224275 23.08077180693529 16 25.74445485659642 25.64903067796559 25.274294077951264 23.332220387486725 17 23.47343954287928 25.52832389069133 27.34805013477959 23.6501864316498 18 25.123637266118244 23.869205795767982 28.645609489853776 22.361547448259998 19 24.055183212583902 27.82991027784308 25.77950726968404 22.335399239888982 20 24.117327210603676 23.819832761513158 28.857768599960252 23.205071427922917 21 25.35507680635678 25.723723105668515 24.155311674606587 24.76588841336812 22 23.723484336308726 26.197909624244968 25.491464332635733 24.587141706810574 23 22.84520812628329 25.47954051286191 25.479036025446344 26.196215335408457 24 22.7826223821732 27.46542607126966 25.46065781017155 24.291293736385587 25 25.07612123706847 24.76598646868083 24.895629662362715 25.26226263188799 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 294.0 1 294.0 2 708.0 3 1122.0 4 1122.0 5 1122.0 6 3050.5 7 4979.0 8 4979.0 9 4979.0 10 4753.0 11 4527.0 12 4527.0 13 4527.0 14 4008.0 15 3489.0 16 3489.0 17 3489.0 18 5602.0 19 7715.0 20 7715.0 21 7715.0 22 11937.5 23 16160.0 24 16160.0 25 16160.0 26 23800.5 27 31441.0 28 31441.0 29 31441.0 30 38502.0 31 45563.0 32 45563.0 33 45563.0 34 52625.0 35 59687.0 36 59687.0 37 59687.0 38 67073.5 39 74460.0 40 74460.0 41 74460.0 42 90345.0 43 106230.0 44 106230.0 45 106230.0 46 142713.0 47 179196.0 48 179196.0 49 179196.0 50 156594.0 51 133992.0 52 133992.0 53 133992.0 54 115999.0 55 98006.0 56 98006.0 57 98006.0 58 89462.0 59 80918.0 60 80918.0 61 80918.0 62 71189.0 63 61460.0 64 61460.0 65 61460.0 66 50847.5 67 40235.0 68 40235.0 69 40235.0 70 30170.5 71 20106.0 72 20106.0 73 20106.0 74 15554.0 75 11002.0 76 11002.0 77 11002.0 78 8951.0 79 6900.0 80 6900.0 81 6900.0 82 4820.0 83 2740.0 84 2740.0 85 2740.0 86 1996.0 87 1252.0 88 1252.0 89 1252.0 90 829.0 91 406.0 92 406.0 93 406.0 94 257.0 95 108.0 96 108.0 97 108.0 98 282.0 99 456.0 100 456.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007456340105839725 2 0.003425885994575009 3 0.004231976816827952 4 0.012494407744920619 5 0.03335200777071553 6 0.04362966575444055 7 0.06448726578023546 8 0.08524410445324875 9 0.09562252378975539 10 0.11466641946548117 11 0.1164801238155503 12 0.1293775769715974 13 0.11688316922667677 14 0.11920068034065398 15 0.11164357888203263 16 0.10892302235692897 17 0.12051057792681502 18 0.12474255474364297 19 0.1282692020909996 20 0.11738697599058487 21 0.12595169097702238 22 0.14368568906658713 23 0.13491945137458639 24 0.13169508808557462 25 0.12716082721040178 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 992444.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.26799649058268 #Duplication Level Percentage of deduplicated Percentage of total 1 78.73811345911352 38.79269097578707 2 12.66992690846586 12.4844382892447 3 3.8000203368882137 5.616581658658539 4 1.5750412506896727 3.1039650724600705 5 0.8322039156467204 2.0500509797765893 6 0.5078161446568263 1.5011450419688246 7 0.3171281219017744 1.0936987037845187 8 0.23711406693151751 0.9345708013959835 9 0.1720300241473763 0.762801716337101 >10 0.9374837984279633 8.642508245979554 >50 0.0957732259818986 3.321189389532976 >100 0.10255170727421446 9.594416176531642 >500 0.009659178806929288 3.348026685769205 >1k 0.0045213177394137095 5.458338552484314 >5k 2.0551444270062316E-4 0.5900003477280941 >10k+ 4.110288854012463E-4 2.7055773625609065 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGAAGCAGTGGTATCAACG 14526 1.4636594105057819 No Hit TCCATGTACTCTGCGTTGATACCAC 12195 1.2287846971718304 No Hit GAGTACATGGAAGCAGTGGTATCAA 5827 0.5871364026584875 No Hit CATGTACTCTGCGTTGATACCACTG 4847 0.48839027693250203 No Hit GTATCAACGCAGAGTACTTTTTTTT 4349 0.4382111232472563 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3688 0.3716078690586068 No Hit GCTTCCATGTACTCTGCGTTGATAC 3545 0.35719899561083546 No Hit TATCAACGCAGAGTACTTTTTTTTT 3259 0.32838124871529273 No Hit CATGGAAGCAGTGGTATCAACGCAG 3159 0.31830511343713097 No Hit GCGTTGATACCACTGCTTCCATGTA 3126 0.31497998879533756 No Hit CCCATGTACTCTGCGTTGATACCAC 3042 0.3065160351616817 No Hit GTACATGGGAAGCAGTGGTATCAAC 2801 0.28223254914131174 No Hit GTATCAACGCAGAGTACATGGAAGC 2551 0.25704221094590723 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2456 0.24746988243165355 No Hit ACTCTGCGTTGATACCACTGCTTCC 2100 0.2115988408413976 No Hit ACGCAGAGTACATGGAAGCAGTGGT 2022 0.2037394553244314 No Hit CAGTGGTATCAACGCAGAGTACATG 1875 0.1889275364655336 No Hit TATCAACGCAGAGTACATGGAAGCA 1766 0.17794454901233722 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1725 0.17381333354829087 No Hit GGTATCAACGCAGAGTACTTTTTTT 1507 0.15184735864189816 No Hit GTACATGGGGTGGTATCAACGCAAA 1417 0.14277883689155257 No Hit GTATCAACGCAGAGTACATGGGAAG 1396 0.1406628484831386 No Hit ATACCACTGCTTCCATGTACTCTGC 1152 0.11607707840442381 No Hit GGTATCAACGCAGAGTACATGGAAG 1121 0.11295347646819366 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1004 0.10116439819274439 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.007613527816179E-4 2 0.0 0.0 0.0 0.0 1.007613527816179E-4 3 0.0 0.0 0.0 0.0 1.007613527816179E-4 4 0.0 0.0 0.0 0.0 1.007613527816179E-4 5 0.0 0.0 0.0 0.0 1.007613527816179E-4 6 0.0 0.0 0.0 0.0 1.007613527816179E-4 7 0.0 0.0 0.0 0.0 1.007613527816179E-4 8 0.0 0.0 0.0 0.0 1.007613527816179E-4 9 0.0 0.0 0.0 0.0 1.007613527816179E-4 10 0.0 0.0 0.0 0.0 1.007613527816179E-4 11 0.0 0.0 0.0 0.0 1.007613527816179E-4 12 0.0 0.0 0.0 0.0 1.007613527816179E-4 13 0.0 0.0 0.0 1.007613527816179E-4 1.007613527816179E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 105 5.456968E-12 15.372786 1 TGGGTTA 45 6.773577E-4 14.773657 5 GTTTAAG 65 3.3877623E-6 14.607626 1 TAGTACT 75 9.692958E-7 13.929448 4 CGAGCCG 200 0.0 13.777415 15 TTAAGAC 70 7.2809216E-6 13.56696 3 CGTTATT 80 2.0069892E-6 13.056881 2 CTCGTAG 60 4.0923568E-4 12.665691 10 CGTCGTA 90 5.403381E-7 12.665691 10 AACTCGG 60 4.0923568E-4 12.665691 10 GCTCGTA 60 4.0923568E-4 12.665691 9 GTATAGT 60 4.1080633E-4 12.659942 1 AGGCCCG 190 0.0 12.499038 10 CGCCAGT 100 1.4314173E-7 12.355907 18 GAGCCGC 240 0.0 12.275464 16 CGACCCG 70 1.09297325E-4 12.21088 5 GCGTTAT 70 1.09555E-4 12.2078 1 CTATTCC 195 0.0 12.176091 4 CCTATTC 180 0.0 12.1348915 3 GTAGTAC 55 0.003074671 12.086928 3 >>END_MODULE