FastQCFastQC Report
Thu 2 Feb 2017
SRR4062455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992444
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA65170.6566617360778039No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC57800.5824006190777515No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA24330.24515237131767634No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC21880.22046583988618No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG14270.14378645041936874No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14110.14217426877486286No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG13630.1373377238413452No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC13590.13693467843021873No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA13470.1357255421968393No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11400.1148679421710444No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11100.11184510158759586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.11093824941256132No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10260.10338114795393996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAA450.001398103124.44424634
TAACGGC852.3519533E-723.29393236
TTAACGG852.3519533E-723.29393235
AACGTAT602.8724322E-421.99982531
CGGTTCG500.002580235221.99982539
TATGCGT1201.4308171E-820.16650639
TAGGACG1750.020.1141244
CGCGGGA2150.019.44170444
TGGTATA4000.019.24984744
ACGTATG808.9870584E-519.24984632
CTAGCGG3050.018.75394829
CGGTCCA3200.018.56235110
TCTAGCG3100.018.45146628
GATATAC2450.017.9644721
AAACGCT2450.017.9590429
TTGGACA1601.1004886E-917.8748574
TAGCGGC3200.017.87485730
GTATAAG2100.017.8147681
GTCTATA750.001288105217.6051831
TAGCCCA1002.4824578E-517.599864
Produced by FastQC (version 0.11.5)