##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992444
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.129368508449847	32.0	32.0	32.0	32.0	32.0
2	31.39129865261919	32.0	32.0	32.0	32.0	32.0
3	31.47047490840793	32.0	32.0	32.0	32.0	32.0
4	31.589383380825517	32.0	32.0	32.0	32.0	32.0
5	31.514158985292873	32.0	32.0	32.0	32.0	32.0
6	35.10005602331215	36.0	36.0	36.0	36.0	36.0
7	35.11175340875656	36.0	36.0	36.0	36.0	36.0
8	35.06238034589357	36.0	36.0	36.0	36.0	36.0
9	35.16307217334177	36.0	36.0	36.0	36.0	36.0
10	35.06563594520195	36.0	36.0	36.0	36.0	36.0
11	35.17461035584879	36.0	36.0	36.0	36.0	36.0
12	35.08576504064713	36.0	36.0	36.0	36.0	36.0
13	35.1181154805712	36.0	36.0	36.0	36.0	36.0
14	35.077318216443444	36.0	36.0	36.0	36.0	36.0
15	35.04701827004849	36.0	36.0	36.0	36.0	36.0
16	35.04616985945807	36.0	36.0	36.0	36.0	36.0
17	35.01984998649798	36.0	36.0	36.0	36.0	36.0
18	35.027727509058444	36.0	36.0	36.0	36.0	36.0
19	34.98349529041437	36.0	36.0	36.0	36.0	36.0
20	34.97065527122941	36.0	36.0	36.0	36.0	36.0
21	34.91813341609199	36.0	36.0	36.0	32.0	36.0
22	34.890244688869096	36.0	36.0	36.0	32.0	36.0
23	34.82134911390467	36.0	36.0	36.0	32.0	36.0
24	34.7976308990734	36.0	36.0	36.0	32.0	36.0
25	34.771368460084396	36.0	36.0	36.0	32.0	36.0
26	34.71452696575323	36.0	36.0	36.0	32.0	36.0
27	34.68116790468782	36.0	36.0	36.0	32.0	36.0
28	34.639118176945	36.0	36.0	36.0	32.0	36.0
29	34.59650720846718	36.0	36.0	36.0	32.0	36.0
30	34.5672934694552	36.0	36.0	36.0	32.0	36.0
31	34.55596285533491	36.0	36.0	36.0	32.0	36.0
32	34.4934797328615	36.0	36.0	36.0	32.0	36.0
33	34.4374231694685	36.0	36.0	36.0	32.0	36.0
34	34.3954973781896	36.0	36.0	36.0	32.0	36.0
35	34.3026951646642	36.0	36.0	36.0	32.0	36.0
36	34.260137599703356	36.0	36.0	36.0	32.0	36.0
37	34.18482957224791	36.0	36.0	36.0	32.0	36.0
38	34.10733200059651	36.0	36.0	36.0	32.0	36.0
39	33.952541402839856	36.0	36.0	36.0	32.0	36.0
40	33.85944798900492	36.0	36.0	36.0	32.0	36.0
41	33.85744183047104	36.0	36.0	36.0	32.0	36.0
42	33.6827428046318	36.0	36.0	36.0	27.0	36.0
43	33.71872569132364	36.0	36.0	36.0	27.0	36.0
44	33.7034341484255	36.0	36.0	36.0	27.0	36.0
45	33.49149070375759	36.0	36.0	36.0	21.0	36.0
46	33.612293489607474	36.0	36.0	36.0	27.0	36.0
47	33.48235870235499	36.0	36.0	36.0	21.0	36.0
48	33.46918113263821	36.0	36.0	36.0	21.0	36.0
49	33.512078263357935	36.0	36.0	36.0	21.0	36.0
50	32.87742784479527	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	12.0
21	24.0
22	106.0
23	365.0
24	1045.0
25	2585.0
26	5532.0
27	10611.0
28	17272.0
29	24410.0
30	32537.0
31	43384.0
32	61753.0
33	84017.0
34	166016.0
35	542774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67664629623658	17.64195475507111	12.569334709277951	25.112064239414362
2	14.641993794834537	21.48923971104364	36.79558322811692	27.073183266004904
3	18.632039587239944	26.097370019981035	28.53459298839931	26.73599740437971
4	12.176630976923589	16.66142706576304	35.151374085336975	36.0105678719764
5	13.388967034915824	37.63134242335084	33.7736940321066	15.20599650962674
6	33.680422956280616	34.513014853245984	17.685751221984535	14.12081096848887
7	28.594963544542562	31.0734913002648	21.921639911168793	18.409905244023843
8	28.81371644143146	32.180455521923655	20.616578869941275	18.38924916670361
9	27.308644114932427	14.803253382558612	18.5115734489805	39.37652905352846
10	15.646726666693537	27.637629931764412	32.30600416748955	24.409639234052502
11	36.64267202985761	21.53562316866241	21.40161056946286	20.42009423201712
12	23.330585907114155	24.45397422927641	29.705454413548775	22.509985450060658
13	30.871364026584875	19.90238240142517	25.262684846701678	23.96356872528828
14	24.358452466839438	19.216802157099043	25.36939111929741	31.05535425676411
15	25.798735243499888	26.147671808182626	24.928459439525053	23.125133508792437
16	25.772134246365535	25.360725642958194	25.340875656460216	23.526264454216054
17	23.57765274413468	25.449496394758796	27.353684439625813	23.61916642148071
18	25.352160927971756	23.59881262821882	28.67385968377057	22.37516676003885
19	24.26756572663042	27.56336881476436	25.798634482147104	22.370430976458117
20	24.15924727239018	23.573622290023415	28.96102953919818	23.30610089838822
21	25.430754783141417	25.614039683851182	24.289027894772904	24.666177638234497
22	23.845778703886566	25.776970791299053	25.650112248147	24.727138256667377
23	22.95928032211389	24.870723184381184	25.7224588994442	26.447537594060723
24	22.997670397523688	27.132614031622943	25.616357194965154	24.25335837588821
25	25.270544232218644	24.20469064249469	25.048163926629613	25.47660119865705
26	22.821640314214203	24.96745408305154	26.67999403492792	25.530911567806346
27	25.44032711165567	25.85626997593819	25.959852646597692	22.743550265808448
28	24.031280354357527	23.837818557016817	26.60674053145568	25.524160557169978
29	23.045632801447738	25.941211796333093	27.601758890174153	23.411396512045012
30	25.0323443942429	24.614184780199185	27.13694676979255	23.216524055765365
31	22.968852650628147	25.27366783415487	26.17669107778373	25.580788437433245
32	23.119692395742227	25.900907255220446	26.243898900089075	24.735501448948252
33	23.02346530383578	25.212505693016436	26.334483356239748	25.429545646908036
34	22.78717993156289	26.25488188754227	27.701008822664047	23.256929358230792
35	25.205049352910592	25.263692460229493	26.404411735070187	23.126846451789724
36	23.79852162943199	26.418115279048493	26.142432217837985	23.640930873681537
37	25.22358944182241	25.871484940208212	26.2081286198516	22.696796998117776
38	23.922760377411723	25.7548032936871	25.220868885297303	25.101567443603873
39	23.507522842598675	25.51499127406685	26.686845806917063	24.29064007641741
40	25.113054237820975	25.527687204517335	25.316088363675938	24.043170193985755
41	22.51814711963597	25.700190640479466	27.08938741127963	24.692274828604937
42	25.676511722575786	25.91299861755424	26.09588047285288	22.3146091870171
43	24.358150182781095	24.144636876236845	25.5755488470886	25.921664093893458
44	23.75499272503033	25.12877300885491	27.444772702540394	23.67146156357437
45	24.466670159726895	25.366469040066747	26.060815522084873	24.106045278121485
46	23.308341133949252	24.963952589720517	27.33900082926677	24.388705447063458
47	24.410546086227537	24.487326237047128	27.728315149267868	23.373812527457467
48	23.707332605164623	28.053270511988586	24.641088061391876	23.598308821454914
49	22.612258223134	25.527082636400642	28.21720923296428	23.643449907501076
50	22.666009701322594	27.985917564955937	24.314871801072506	25.033200932648963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	42.0
1	45.5
2	49.0
3	183.0
4	317.0
5	350.5
6	384.0
7	354.5
8	325.0
9	425.5
10	526.0
11	774.0
12	1022.0
13	1811.5
14	2601.0
15	3568.0
16	4535.0
17	5215.5
18	5896.0
19	6333.5
20	6771.0
21	7396.0
22	8021.0
23	8396.5
24	8772.0
25	8735.0
26	8698.0
27	10086.0
28	11474.0
29	13030.0
30	14586.0
31	15916.0
32	17246.0
33	19722.5
34	22199.0
35	24891.5
36	27584.0
37	31364.0
38	35144.0
39	37960.0
40	40776.0
41	44830.0
42	48884.0
43	52846.0
44	56808.0
45	67853.0
46	78898.0
47	87209.0
48	95520.0
49	90795.5
50	86071.0
51	76138.0
52	66205.0
53	62138.0
54	58071.0
55	58759.5
56	59448.0
57	57855.0
58	56262.0
59	51613.0
60	46964.0
61	41132.5
62	35301.0
63	30625.5
64	25950.0
65	21915.5
66	17881.0
67	15592.5
68	13304.0
69	12262.5
70	11221.0
71	8733.0
72	6245.0
73	5505.0
74	4765.0
75	3609.5
76	2454.0
77	2182.5
78	1911.0
79	1650.5
80	1390.0
81	1086.5
82	783.0
83	676.0
84	569.0
85	460.5
86	352.0
87	251.0
88	150.0
89	91.0
90	32.0
91	22.5
92	13.0
93	11.0
94	9.0
95	6.5
96	4.0
97	5.0
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015718771033932395
2	0.004534260875172806
3	3.022840583448537E-4
4	2.015227055632358E-4
5	0.0
6	1.007613527816179E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.007613527816179E-4
47	0.0
48	0.0
49	0.0
50	2.015227055632358E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	992444.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.381118551300034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91749843379061	44.259204550850924
2	12.167920823715876	13.477461313220825
3	3.512950284494859	5.836533485112989
4	1.4515585681117553	3.215557485790098
5	0.7493866256329298	2.0750934777467993
6	0.4517805574941312	1.5012067568252943
7	0.30618916112855993	1.1869968762108718
8	0.21876730741651643	0.9692462549746237
9	0.16977495394138153	0.8462094166142239
>10	0.869860790129544	8.924169344056962
>50	0.09124345226303922	3.5357118100631753
>100	0.08425777183880723	8.907631694183719
>500	0.006424884405673193	2.3979035143119227
>1k	0.002019249384640146	1.6169354048896059
>5k	3.671362517527538E-4	1.2501386151479084
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6517	0.6566617360778039	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5780	0.5824006190777515	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2433	0.24515237131767634	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2188	0.22046583988618	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1427	0.14378645041936874	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1411	0.14217426877486286	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1363	0.1373377238413452	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1359	0.13693467843021873	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1347	0.1357255421968393	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1140	0.1148679421710444	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1110	0.11184510158759586	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1101	0.11093824941256132	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1026	0.10338114795393996	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.007613527816179E-4
2	0.0	0.0	0.0	0.0	1.007613527816179E-4
3	0.0	0.0	0.0	0.0	1.007613527816179E-4
4	0.0	0.0	0.0	0.0	1.007613527816179E-4
5	0.0	0.0	0.0	0.0	1.007613527816179E-4
6	0.0	0.0	0.0	0.0	1.007613527816179E-4
7	0.0	0.0	0.0	0.0	1.007613527816179E-4
8	0.0	0.0	0.0	0.0	1.007613527816179E-4
9	0.0	0.0	0.0	0.0	1.007613527816179E-4
10	0.0	0.0	0.0	0.0	1.007613527816179E-4
11	0.0	0.0	0.0	0.0	1.007613527816179E-4
12	0.0	0.0	0.0	0.0	1.007613527816179E-4
13	0.0	0.0	0.0	1.007613527816179E-4	1.007613527816179E-4
14	0.0	0.0	0.0	2.015227055632358E-4	1.007613527816179E-4
15	0.0	0.0	0.0	2.015227055632358E-4	1.007613527816179E-4
16	0.0	0.0	0.0	3.0228405834485374E-4	1.007613527816179E-4
17	0.0	0.0	0.0	3.0228405834485374E-4	1.007613527816179E-4
18	0.0	0.0	0.0	4.030454111264716E-4	1.007613527816179E-4
19	0.0	0.0	0.0	8.060908222529432E-4	1.007613527816179E-4
20	0.0	0.0	0.0	9.068521750345612E-4	1.007613527816179E-4
21	0.0	0.0	0.0	0.0017129429972875044	1.007613527816179E-4
22	0.0	0.0	0.0	0.0026197951723220653	1.007613527816179E-4
23	0.0	0.0	0.0	0.005844158461333839	1.007613527816179E-4
24	0.0	0.0	0.0	0.009270044455908847	1.007613527816179E-4
25	0.0	0.0	0.0	0.012393646392139003	1.007613527816179E-4
26	0.0	0.0	0.0	0.0163233391506221	1.007613527816179E-4
27	0.0	0.0	0.0	0.022469781670300792	1.007613527816179E-4
28	0.0	0.0	0.0	0.034258859945750085	1.007613527816179E-4
29	0.0	0.0	0.0	0.05390732373816558	1.007613527816179E-4
30	0.0	0.0	0.0	0.08685628609775463	1.007613527816179E-4
31	0.0	0.0	0.0	0.15748999439766878	1.007613527816179E-4
32	0.0	0.0	0.0	0.23678917903680208	1.007613527816179E-4
33	0.0	0.0	0.0	0.3065160351616817	1.007613527816179E-4
34	0.0	0.0	0.0	0.3980073434873907	1.007613527816179E-4
35	0.0	0.0	0.0	0.4947382421577439	1.007613527816179E-4
36	0.0	0.0	0.0	0.6425551466883774	1.007613527816179E-4
37	0.0	0.0	0.0	0.8788405189612714	1.007613527816179E-4
38	0.0	0.0	0.0	1.1219776632233154	1.007613527816179E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCAA	45	0.0013981031	24.444246	34
TAACGGC	85	2.3519533E-7	23.293932	36
TTAACGG	85	2.3519533E-7	23.293932	35
AACGTAT	60	2.8724322E-4	21.999825	31
CGGTTCG	50	0.0025802352	21.999825	39
TATGCGT	120	1.4308171E-8	20.166506	39
TAGGACG	175	0.0	20.114124	4
CGCGGGA	215	0.0	19.441704	44
TGGTATA	400	0.0	19.249847	44
ACGTATG	80	8.9870584E-5	19.249846	32
CTAGCGG	305	0.0	18.753948	29
CGGTCCA	320	0.0	18.562351	10
TCTAGCG	310	0.0	18.451466	28
GATATAC	245	0.0	17.964472	1
AAACGCT	245	0.0	17.95904	29
TTGGACA	160	1.1004886E-9	17.874857	4
TAGCGGC	320	0.0	17.874857	30
GTATAAG	210	0.0	17.814768	1
GTCTATA	75	0.0012881052	17.605183	1
TAGCCCA	100	2.4824578E-5	17.59986	4
>>END_MODULE