FastQCFastQC Report
Thu 2 Feb 2017
SRR4062454_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062454_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920100
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCA31800.16561637414717983No Hit
GCGCAAGACGGACCAGAGCGAAAGC30120.15686682985261183No Hit
GTATTAGAGGCACTGCCTGCCCAGT29890.15566897557418885No Hit
GTATCAACGCAGAGTACTTTTTTTT29840.15540857247018383No Hit
CCATAGGGTCTTCTCGTCTTATTAT29520.15374199260455185No Hit
GTCTTGCGCCGGTCCAAGAATTTCA27630.14389875527316284No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC27540.14343002968595386No Hit
GAATAGGACCGCGGTTCTATTTTGT26830.13973230560908287No Hit
GTACATGGGGAATAATTGCAATCCC26350.13723243581063488No Hit
GATTAAGAGGGACGGCCGGGGGCAT25830.13452424352898287No Hit
TCGTAGTTCCGACCATAAACGATGC25250.13150356752252487No Hit
GAACTACGACGGTATCTGATCGTCT24960.12999322951929587No Hit
GAATAACGCCGCCGCATCGCCAGTC24960.12999322951929587No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24690.12858705275766885No Hit
GTGCATGGCCGTTCTTAGTTGGTGG24660.12843081089526587No Hit
GTATCTGATCGTCTTCGAACCTCCG24530.12775376282485287No Hit
CTCTAATACTTGTAATGCTAGAGGT23380.12176449143273788No Hit
GAATAATTGCAATCCCCGATCCCCA22800.11874381542627988No Hit
CATCTAAGGGCATCACAGACCTGTT22120.11520233321181189No Hit
ATCAGATACCGTCGTAGTTCCGACC22000.11457736576219989No Hit
CCATTGGGATGTCCTGATCCAACAT20340.1059319827092339No Hit
GTCCTATTCCATTATTCCTAGCTGC20260.10551533774282588No Hit
TATCAACGCAGAGTACTTTTTTTTT19870.10348419353158689No Hit
CTCTTAATCATGGCCTCAGTTCCGA19580.1019738555283579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACCG2300.013.2163035
AACCGCG1950.013.154827
GTTTAGA2550.013.0286291
CGGTCCA5150.012.91391610
ATCCCCG3550.012.84669212
TCGGCAA1501.8189894E-1212.66793613
GAACCGC2200.012.5236566
AACGGAC2750.012.43793515
CGCGCCA1001.4412217E-712.35123710
GAATAAG701.09946224E-412.2043681
AGCCCCG4850.012.14713116
TTCGCAG550.003065993512.09243715
ATCGCCA4250.012.07337316
TAGGGTC5300.012.0075064
CGAGGGA951.0363092E-612.00370819
GACGGCC5100.011.92245311
CGCCAGT4150.011.90729218
ACGGTAT4400.011.876199
TAGAACC2800.011.8730934
TCGCCAG4250.011.85071917
Produced by FastQC (version 0.11.5)