##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062454_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1920100
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.222861309306808	32.0	32.0	32.0	32.0	32.0
2	30.87967345450758	32.0	32.0	32.0	32.0	32.0
3	30.87840633300349	32.0	32.0	32.0	32.0	32.0
4	30.96106556950159	32.0	32.0	32.0	32.0	32.0
5	30.769472944117492	32.0	32.0	32.0	32.0	32.0
6	34.41996510598406	36.0	36.0	36.0	32.0	36.0
7	34.29008801624915	36.0	36.0	36.0	32.0	36.0
8	34.28866881933233	36.0	36.0	36.0	32.0	36.0
9	34.45830633821155	36.0	36.0	36.0	32.0	36.0
10	34.130691630644236	36.0	36.0	36.0	32.0	36.0
11	34.44348158950055	36.0	36.0	36.0	32.0	36.0
12	34.20082808187074	36.0	36.0	36.0	32.0	36.0
13	34.35742200927035	36.0	36.0	36.0	32.0	36.0
14	34.25584396646008	36.0	36.0	36.0	32.0	36.0
15	34.18957398052185	36.0	36.0	36.0	32.0	36.0
16	34.19301911358784	36.0	36.0	36.0	32.0	36.0
17	34.10597052236863	36.0	36.0	36.0	32.0	36.0
18	34.12962449872403	36.0	36.0	36.0	32.0	36.0
19	34.11134472162908	36.0	36.0	36.0	32.0	36.0
20	34.11935732513932	36.0	36.0	36.0	32.0	36.0
21	34.09366335086714	36.0	36.0	36.0	32.0	36.0
22	34.03546429873444	36.0	36.0	36.0	32.0	36.0
23	34.01491224415395	36.0	36.0	36.0	32.0	36.0
24	33.983520649966145	36.0	36.0	36.0	32.0	36.0
25	33.54889016197073	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	67.0
5	172.0
6	347.0
7	84.0
8	295.0
9	328.0
10	246.0
11	75.0
12	135.0
13	109.0
14	285.0
15	312.0
16	566.0
17	766.0
18	890.0
19	1297.0
20	2084.0
21	3072.0
22	4795.0
23	7144.0
24	10441.0
25	15033.0
26	21100.0
27	27936.0
28	37784.0
29	50897.0
30	66230.0
31	91286.0
32	132722.0
33	193594.0
34	436724.0
35	813272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.241739239134965	19.04563731822159	11.39318304929372	25.319440393349723
2	16.694461342310962	20.780434909418176	37.47291711804803	25.052186630222828
3	18.27936043983173	25.00803382487668	29.189268580791634	27.523337154499956
4	11.553340761124629	16.198939794782525	38.236144382511455	34.01157506158139
5	13.484014189695747	37.911224270235664	34.38026363158473	14.22449790848386
6	31.9339215885496	37.792714284597764	17.724041089288182	12.549323037564447
7	29.338017518267957	31.953562570714883	21.21475070056916	17.493669210448004
8	27.47621470257692	32.856758610767656	20.20900619887699	19.458020487778434
9	27.10614155856951	14.774542957963446	19.997456026532394	38.12185945693465
10	15.811867543287454	26.470902624283106	32.10662737203042	25.610602460399022
11	36.0311585542631	22.475393731580496	23.29190052789545	18.201547186260953
12	23.916784700333555	25.86830363196025	28.722284686774458	21.49262698093174
13	27.401495395913866	20.4052346678639	26.654579604359895	25.53869033186234
14	22.40963767466017	21.968698311122978	25.563741184862888	30.057922829353963
15	24.53253899809898	28.366287539482997	23.042743313829504	24.05843014858852
16	25.12353207089127	27.0519723680252	24.456143442672097	23.36835211841143
17	22.953880008238972	26.915626148828164	25.80028836401655	24.330205478916316
18	23.75181738725941	25.600493953862873	27.611092685185763	23.036595973691952
19	24.25642895026276	26.515889915031625	26.366583799947534	22.861097334758078
20	24.265200491830463	25.785797196065772	25.905104670407887	24.043897641695878
21	26.239100959532752	25.131466416353774	25.078170629954112	23.55126199415937
22	24.794817000497083	25.953699970425482	25.673028926173764	23.578454102903677
23	23.43692059758473	25.62620443990122	26.55163151896985	24.3852434435442
24	23.39679554674556	26.808965126290797	26.047564587365237	23.746674739598408
25	23.404128827425943	26.1417481338088	26.480775909993103	23.973347128772147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	252.0
1	252.0
2	640.5
3	1029.0
4	1029.0
5	1029.0
6	3230.0
7	5431.0
8	5431.0
9	5431.0
10	5902.0
11	6373.0
12	6373.0
13	6373.0
14	7888.5
15	9404.0
16	9404.0
17	9404.0
18	15239.5
19	21075.0
20	21075.0
21	21075.0
22	32867.5
23	44660.0
24	44660.0
25	44660.0
26	65348.5
27	86037.0
28	86037.0
29	86037.0
30	100806.0
31	115575.0
32	115575.0
33	115575.0
34	134801.0
35	154027.0
36	154027.0
37	154027.0
38	163180.0
39	172333.0
40	172333.0
41	172333.0
42	185262.0
43	198191.0
44	198191.0
45	198191.0
46	222762.5
47	247334.0
48	247334.0
49	247334.0
50	238519.0
51	229704.0
52	229704.0
53	229704.0
54	204576.0
55	179448.0
56	179448.0
57	179448.0
58	163758.0
59	148068.0
60	148068.0
61	148068.0
62	132443.5
63	116819.0
64	116819.0
65	116819.0
66	99803.5
67	82788.0
68	82788.0
69	82788.0
70	63076.5
71	43365.0
72	43365.0
73	43365.0
74	35727.0
75	28089.0
76	28089.0
77	28089.0
78	22717.5
79	17346.0
80	17346.0
81	17346.0
82	12178.5
83	7011.0
84	7011.0
85	7011.0
86	5238.5
87	3466.0
88	3466.0
89	3466.0
90	2371.5
91	1277.0
92	1277.0
93	1277.0
94	746.0
95	215.0
96	215.0
97	215.0
98	499.0
99	783.0
100	783.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00937451174417999
2	0.003541482214467996
3	0.0048434977344929955
4	0.014738815686682985
5	0.035362741523878964
6	0.04504973699286496
7	0.06786104890370294
8	0.0879120879120879
9	0.09588042289464091
10	0.11879589604708088
11	0.11957710535909587
12	0.13374303421696787
13	0.12223321701994687
14	0.12374355502317588
15	0.11384823707098587
16	0.11337951148377688
17	0.12421228061038489
18	0.13082651945211188
19	0.13348263111296288
20	0.12322274881516589
21	0.13020155200249986
22	0.15150252591010885
23	0.14139888547471485
24	0.13442008228738087
25	0.13322222800895786
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1920100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.74967855499761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.08289843604312	36.73183319875445
2	11.966436590688177	10.709883817641149
3	2.9132336661550764	3.9109881034811065
4	0.9774001764619091	1.7495337486507347
5	0.43553876521306406	0.9745109870762597
6	0.2591340526014159	0.6957699333940321
7	0.16683670179885032	0.522612214367218
8	0.11858958309266887	0.42454765786944887
9	0.0922974375165589	0.37172525942843887
>10	0.6687427305801663	6.154682498512175
>50	0.1210888570227778	3.890780731976525
>100	0.1566088711907714	15.27128542689804
>500	0.026957066311115756	8.53051052676673
>1k	0.014237065324485378	10.061335895183646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCA	3180	0.16561637414717983	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3012	0.15686682985261183	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	2989	0.15566897557418885	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2984	0.15540857247018383	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	2952	0.15374199260455185	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2763	0.14389875527316284	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2754	0.14343002968595386	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2683	0.13973230560908287	No Hit
GTACATGGGGAATAATTGCAATCCC	2635	0.13723243581063488	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2583	0.13452424352898287	No Hit
TCGTAGTTCCGACCATAAACGATGC	2525	0.13150356752252487	No Hit
GAACTACGACGGTATCTGATCGTCT	2496	0.12999322951929587	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2496	0.12999322951929587	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2469	0.12858705275766885	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2466	0.12843081089526587	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2453	0.12775376282485287	No Hit
CTCTAATACTTGTAATGCTAGAGGT	2338	0.12176449143273788	No Hit
GAATAATTGCAATCCCCGATCCCCA	2280	0.11874381542627988	No Hit
CATCTAAGGGCATCACAGACCTGTT	2212	0.11520233321181189	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2200	0.11457736576219989	No Hit
CCATTGGGATGTCCTGATCCAACAT	2034	0.1059319827092339	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2026	0.10551533774282588	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1987	0.10348419353158689	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1958	0.1019738555283579	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.208062080099995E-5	0.0
3	0.0	0.0	0.0	1.041612416019999E-4	0.0
4	0.0	0.0	0.0	1.5624186240299984E-4	0.0
5	0.0	0.0	0.0	1.5624186240299984E-4	0.0
6	0.0	0.0	0.0	1.5624186240299984E-4	0.0
7	0.0	0.0	0.0	2.083224832039998E-4	5.208062080099995E-5
8	0.0	0.0	0.0	2.083224832039998E-4	5.208062080099995E-5
9	0.0	0.0	0.0	3.124837248059997E-4	5.208062080099995E-5
10	0.0	0.0	0.0	3.124837248059997E-4	5.208062080099995E-5
11	0.0	0.0	0.0	3.124837248059997E-4	5.208062080099995E-5
12	0.0	0.0	0.0	3.124837248059997E-4	5.208062080099995E-5
13	0.0	0.0	0.0	3.645643456069996E-4	5.208062080099995E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAACCG	230	0.0	13.216303	5
AACCGCG	195	0.0	13.15482	7
GTTTAGA	255	0.0	13.028629	1
CGGTCCA	515	0.0	12.913916	10
ATCCCCG	355	0.0	12.846692	12
TCGGCAA	150	1.8189894E-12	12.667936	13
GAACCGC	220	0.0	12.523656	6
AACGGAC	275	0.0	12.437935	15
CGCGCCA	100	1.4412217E-7	12.351237	10
GAATAAG	70	1.09946224E-4	12.204368	1
AGCCCCG	485	0.0	12.147131	16
TTCGCAG	55	0.0030659935	12.092437	15
ATCGCCA	425	0.0	12.073373	16
TAGGGTC	530	0.0	12.007506	4
CGAGGGA	95	1.0363092E-6	12.003708	19
GACGGCC	510	0.0	11.922453	11
CGCCAGT	415	0.0	11.907292	18
ACGGTAT	440	0.0	11.87619	9
TAGAACC	280	0.0	11.873093	4
TCGCCAG	425	0.0	11.850719	17
>>END_MODULE