FastQCFastQC Report
Thu 2 Feb 2017
SRR4062454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920100
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC30270.15764803916462686No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT29580.15405447632935784No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT28570.14879433362845687No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG28420.14801312431644184No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA28180.14676318941721786No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC27840.14499244830998387No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27110.14119056299151087No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT26290.13691995208582886No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA25480.13270142180094785No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25380.13218061559293787No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG24540.12780584344565388No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA24520.12770168220405187No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA24120.12561845737201188No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG23710.12348315191917088No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT23060.12009791156710586No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT22850.1190042185302849No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC22610.11775428363106089No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC22470.11702515493984687No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC20640.1074944013332639No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT19470.1014009686995469No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA19330.1006718400083329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG1351.1277734E-1021.1850595
GTATCAA40100.020.686941
TCGACCA550.004484710319.99988220
CGCGCCA2100.019.90464410
ACCGTAC708.126024E-418.8570338
TAGGACG4450.018.2920274
TCGGCAA4000.018.14989335
GTCCTAT7100.017.9751
CGTCGTA7550.017.4833410
TACCGTC7400.017.2431417
AATCACG2850.016.98235534
GGTATCA20900.016.9503571
TACGACG7550.016.9005625
GTATTAG10850.016.8324621
ACCGTCG8150.016.7360978
GGCCGCG9650.016.64238740
CGGCCGC10050.016.63671939
CGAGGGA2251.8189894E-1216.62212419
TAACGGC9450.016.52900336
ATCACGG2800.016.49990335
Produced by FastQC (version 0.11.5)