##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062454_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1920100
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.337875631477527	32.0	32.0	32.0	32.0	32.0
2	31.464329982813396	32.0	32.0	32.0	32.0	32.0
3	31.531965001822822	32.0	32.0	32.0	32.0	32.0
4	31.62181136399146	32.0	32.0	32.0	32.0	32.0
5	31.57009426592365	32.0	32.0	32.0	32.0	32.0
6	35.159865631998336	36.0	36.0	36.0	36.0	36.0
7	35.17013488880787	36.0	36.0	36.0	36.0	36.0
8	35.11124056038748	36.0	36.0	36.0	36.0	36.0
9	35.24561481172856	36.0	36.0	36.0	36.0	36.0
10	35.130101557210565	36.0	36.0	36.0	36.0	36.0
11	35.2502666527785	36.0	36.0	36.0	36.0	36.0
12	35.15464663298786	36.0	36.0	36.0	36.0	36.0
13	35.21739857299099	36.0	36.0	36.0	36.0	36.0
14	35.177331909796365	36.0	36.0	36.0	36.0	36.0
15	35.13829956773085	36.0	36.0	36.0	36.0	36.0
16	35.14956356439769	36.0	36.0	36.0	36.0	36.0
17	35.11774230508828	36.0	36.0	36.0	36.0	36.0
18	35.1393370136972	36.0	36.0	36.0	36.0	36.0
19	35.11904015415864	36.0	36.0	36.0	36.0	36.0
20	35.107662100932245	36.0	36.0	36.0	36.0	36.0
21	35.10874069058903	36.0	36.0	36.0	36.0	36.0
22	35.093521691578566	36.0	36.0	36.0	36.0	36.0
23	35.05767772511848	36.0	36.0	36.0	36.0	36.0
24	35.03512837873028	36.0	36.0	36.0	36.0	36.0
25	35.013196187698554	36.0	36.0	36.0	36.0	36.0
26	34.974352377480336	36.0	36.0	36.0	36.0	36.0
27	34.95122024894537	36.0	36.0	36.0	36.0	36.0
28	34.945960627050674	36.0	36.0	36.0	32.0	36.0
29	34.923386802770686	36.0	36.0	36.0	32.0	36.0
30	34.906714233633664	36.0	36.0	36.0	32.0	36.0
31	34.908438622988385	36.0	36.0	36.0	32.0	36.0
32	34.87300921826988	36.0	36.0	36.0	32.0	36.0
33	34.864323733138896	36.0	36.0	36.0	32.0	36.0
34	34.84721420759335	36.0	36.0	36.0	32.0	36.0
35	34.82637623040467	36.0	36.0	36.0	32.0	36.0
36	34.7929909900526	36.0	36.0	36.0	32.0	36.0
37	34.7942070725483	36.0	36.0	36.0	32.0	36.0
38	34.75736576219988	36.0	36.0	36.0	32.0	36.0
39	34.75750117181397	36.0	36.0	36.0	32.0	36.0
40	34.75222957137649	36.0	36.0	36.0	32.0	36.0
41	34.71034894015936	36.0	36.0	36.0	32.0	36.0
42	34.69569918233425	36.0	36.0	36.0	32.0	36.0
43	34.69361699911463	36.0	36.0	36.0	32.0	36.0
44	34.65360397895943	36.0	36.0	36.0	32.0	36.0
45	34.62252747252747	36.0	36.0	36.0	32.0	36.0
46	34.62222175928337	36.0	36.0	36.0	32.0	36.0
47	34.602202489453674	36.0	36.0	36.0	32.0	36.0
48	34.55120618717775	36.0	36.0	36.0	32.0	36.0
49	34.54617311598354	36.0	36.0	36.0	32.0	36.0
50	34.038318316754335	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	13.0
21	48.0
22	165.0
23	421.0
24	1129.0
25	2670.0
26	5269.0
27	9816.0
28	16914.0
29	26642.0
30	39394.0
31	56547.0
32	85076.0
33	142507.0
34	323952.0
35	1209530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62696083155145	19.71236362405165	11.938941079339614	25.721734465057285
2	16.080038999715626	21.354479706918802	37.184780944305615	25.380700349059953
3	17.646225525784835	25.260796153497612	29.57316406278482	27.519814257932733
4	11.241862402999844	16.719493776365816	38.39987500651007	33.63876881412426
5	13.14374251341076	38.2445185146607	34.3122754023228	14.29946356960575
6	31.939007057478	38.08319501523626	17.463286896298143	12.514511030987594
7	29.156502265507005	32.04015415863757	21.249830737982396	17.55351283787303
8	27.35916220986522	33.09850169183985	20.109483938067775	19.432852160227153
9	27.13082651945211	14.642987344409145	19.926878808395397	38.29930732774335
10	15.814124264361231	26.558981303057134	32.09478672985782	25.532107702723817
11	36.12764960158325	22.377896984532057	23.344669548461017	18.14978386542368
12	23.76730378626113	25.913181605124734	28.73782615488777	21.58168845372637
13	27.58788604760169	20.248841206187176	26.650434873183688	25.512837873027443
14	22.44674756523098	21.904952867038176	25.567001718660485	30.08129784907036
15	24.45924691422322	28.394510702567576	22.982552992031664	24.16368939117754
16	25.1929066194469	27.020415603353992	24.37711577522004	23.409562001979065
17	23.046976719962505	26.891047341284306	25.722618613613875	24.339357325139314
18	23.893495130461957	25.512525389302642	27.53814905473673	23.055830425498673
19	24.362064475808552	26.421175980417686	26.360345815322116	22.856413728451646
20	24.300869746367376	25.71324410186969	25.883235248164162	24.10265090359877
21	26.392635800218738	25.09171397323056	25.109733868027707	23.405916358522994
22	24.799645851778553	25.890526535076297	25.645278891724388	23.664548721420758
23	23.440445810114056	25.45705952814958	26.591115046091353	24.51137961564502
24	23.400187490234885	26.71131711890006	26.058017811572316	23.830477579292744
25	23.52559762512369	25.907400656215824	26.52538930264049	24.04161241602
26	22.994062809228684	26.81933232644133	27.276912660798917	22.909692203531066
27	24.552158741732203	26.10608822457164	25.65210145304932	23.689651580646842
28	22.920160408312068	25.981094734649236	27.266809020363524	23.83193583667517
29	23.269569293265977	26.066715275246082	26.814697151190042	23.8490182802979
30	23.34852351440029	26.142492578511533	27.260871829592208	23.248112077495964
31	23.819905213270143	25.82235300244779	26.09582834227384	24.26191344200823
32	23.322639445862194	26.030571324410186	26.175355450236964	24.47143377949065
33	23.11551481693662	25.61840529139107	26.614811728555804	24.651268163116505
34	23.874485703869592	25.75891880631217	27.260403104005	23.10619238581324
35	24.925316389771364	25.495911671267123	26.389354721108276	23.189417217853237
36	22.932034789854693	25.904848705796574	26.606322587365238	24.55679391698349
37	25.020728087078798	25.83021717618874	25.386177803239413	23.762876933493047
38	22.962501953023278	25.699911462944634	26.86672569137024	24.470860892661843
39	23.85979896880371	26.05890318212593	25.370553616999114	24.710744232071246
40	24.007082964428935	25.896099161502008	26.94932555596063	23.14749231810843
41	23.13389927607937	26.420238529243267	26.992969116191865	23.452893078485495
42	24.442008228738086	26.670850476537684	26.134159679183377	22.752981615540858
43	23.27253788865163	25.502838393833656	26.588771418155304	24.63585229935941
44	23.077183480027085	26.035779386490287	26.440445810114056	24.446591323368576
45	23.395083589396386	26.258163637310556	26.697203270662982	23.64954950263007
46	23.54037322072791	25.792551105491025	26.34648145380155	24.32059421997951
47	23.71767095463778	25.282849851570234	26.89099526066351	24.108483933128483
48	24.070465079943755	26.581375970001563	25.78433414926306	23.563824800791625
49	22.651320243737306	26.467996458517785	26.547783969584916	24.33289932815999
50	22.316129368262068	26.68985990313005	26.484245612207697	24.509765116400185
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	38.5
2	40.0
3	124.0
4	208.0
5	237.0
6	266.0
7	237.5
8	209.0
9	315.0
10	421.0
11	660.0
12	899.0
13	1605.5
14	2312.0
15	3088.5
16	3865.0
17	5271.5
18	6678.0
19	7408.5
20	8139.0
21	10491.5
22	12844.0
23	13212.5
24	13581.0
25	18771.5
26	23962.0
27	31254.0
28	38546.0
29	42733.5
30	46921.0
31	46151.5
32	45382.0
33	51058.5
34	56735.0
35	64918.0
36	73101.0
37	88572.0
38	104043.0
39	106425.5
40	108808.0
41	116490.5
42	124173.0
43	117225.5
44	110278.0
45	116496.5
46	122715.0
47	125769.5
48	128824.0
49	130836.0
50	132848.0
51	121788.0
52	110728.0
53	105892.5
54	101057.0
55	103134.0
56	105211.0
57	104241.5
58	103272.0
59	93676.0
60	84080.0
61	73068.0
62	62056.0
63	55383.0
64	48710.0
65	43073.5
66	37437.0
67	34818.5
68	32200.0
69	29851.0
70	27502.0
71	20486.5
72	13471.0
73	12191.0
74	10911.0
75	7994.5
76	5078.0
77	4704.5
78	4331.0
79	3789.0
80	3247.0
81	2584.0
82	1921.0
83	1677.5
84	1434.0
85	1191.0
86	948.0
87	715.0
88	482.0
89	309.5
90	137.0
91	82.0
92	27.0
93	18.0
94	9.0
95	7.0
96	5.0
97	4.0
98	3.0
99	5.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01640539555231498
2	0.0044789333888859955
3	9.374511744179991E-4
4	0.0
5	0.0
6	4.6872558720899955E-4
7	0.0
8	5.2080620800999944E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5624186240299984E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1920100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.98022996401555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48183330882351	39.574973300109505
2	11.810461226779385	11.333372912839284
3	2.8291871451956467	4.072351495131714
4	0.923112460497484	1.7716459254927
5	0.4240826398045371	1.017379129078423
6	0.2584465638947619	0.7440195341448189
7	0.16269974650798522	0.5464459876778117
8	0.12066210453377786	0.46315164187781965
9	0.08377931856249118	0.3617775873771159
>10	0.628930624974639	6.157588080606063
>50	0.10884981444604447	3.7251104891199898
>100	0.13286035968413523	13.76746675776827
>500	0.02386874626937599	7.9931157573595115
>1k	0.011225940026236196	8.471601401417054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3027	0.15764803916462686	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	2958	0.15405447632935784	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2857	0.14879433362845687	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	2842	0.14801312431644184	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2818	0.14676318941721786	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2784	0.14499244830998387	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2711	0.14119056299151087	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2629	0.13691995208582886	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	2548	0.13270142180094785	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	2538	0.13218061559293787	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2454	0.12780584344565388	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2452	0.12770168220405187	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2412	0.12561845737201188	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2371	0.12348315191917088	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	2306	0.12009791156710586	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	2285	0.1190042185302849	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC	2261	0.11775428363106089	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2247	0.11702515493984687	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	2064	0.1074944013332639	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT	1947	0.1014009686995469	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	1933	0.1006718400083329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.208062080099995E-5	0.0
5	0.0	0.0	0.0	5.208062080099995E-5	0.0
6	0.0	0.0	0.0	5.208062080099995E-5	0.0
7	0.0	0.0	0.0	5.208062080099995E-5	0.0
8	0.0	0.0	0.0	5.208062080099995E-5	0.0
9	0.0	0.0	0.0	5.208062080099995E-5	0.0
10	0.0	0.0	0.0	5.208062080099995E-5	0.0
11	0.0	0.0	0.0	5.208062080099995E-5	0.0
12	0.0	0.0	0.0	5.208062080099995E-5	0.0
13	0.0	0.0	0.0	1.041612416019999E-4	0.0
14	0.0	0.0	0.0	1.5624186240299984E-4	0.0
15	0.0	0.0	0.0	2.6040310400499974E-4	0.0
16	0.0	0.0	0.0	4.166449664079996E-4	0.0
17	0.0	0.0	0.0	5.728868288109994E-4	0.0
18	0.0	0.0	0.0	5.728868288109994E-4	0.0
19	0.0	0.0	0.0	7.291286912139992E-4	0.0
20	5.208062080099995E-5	0.0	0.0	9.89531795218999E-4	0.0
21	5.208062080099995E-5	0.0	0.0	0.001093693036820999	0.0
22	5.208062080099995E-5	0.0	0.0	0.0016665798656319984	0.0
23	5.208062080099995E-5	0.0	0.0	0.0024998697984479974	0.0
24	5.208062080099995E-5	0.0	0.0	0.004218530284880995	0.0
25	5.208062080099995E-5	0.0	0.0	0.004895578355293995	0.0
26	5.208062080099995E-5	0.0	0.0	0.005833029529711994	0.0
27	5.208062080099995E-5	0.0	0.0	0.0077079318785479925	0.0
28	5.208062080099995E-5	0.0	0.0	0.014061767616269986	0.0
29	5.208062080099995E-5	0.0	0.0	0.025311181709285976	0.0
30	5.208062080099995E-5	0.0	0.0	0.05072652466017395	0.0
31	5.208062080099995E-5	0.0	0.0	0.10556741836362689	0.0
32	5.208062080099995E-5	0.0	0.0	0.16764751835841885	0.0
33	5.208062080099995E-5	0.0	0.0	0.23259205249726578	0.0
34	5.208062080099995E-5	0.0	0.0	0.3067548565178897	0.0
35	5.208062080099995E-5	0.0	0.0	0.38872975365866363	0.0
36	5.208062080099995E-5	0.0	0.0	0.5173168064163325	0.0
37	5.208062080099995E-5	0.0	0.0	0.6985052861830113	5.208062080099995E-5
38	5.208062080099995E-5	0.0	0.0	0.93489922399875	5.208062080099995E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	135	1.1277734E-10	21.185059	5
GTATCAA	4010	0.0	20.68694	1
TCGACCA	55	0.0044847103	19.999882	20
CGCGCCA	210	0.0	19.904644	10
ACCGTAC	70	8.126024E-4	18.857033	8
TAGGACG	445	0.0	18.292027	4
TCGGCAA	400	0.0	18.149893	35
GTCCTAT	710	0.0	17.975	1
CGTCGTA	755	0.0	17.48334	10
TACCGTC	740	0.0	17.243141	7
AATCACG	285	0.0	16.982355	34
GGTATCA	2090	0.0	16.950357	1
TACGACG	755	0.0	16.900562	5
GTATTAG	1085	0.0	16.832462	1
ACCGTCG	815	0.0	16.736097	8
GGCCGCG	965	0.0	16.642387	40
CGGCCGC	1005	0.0	16.636719	39
CGAGGGA	225	1.8189894E-12	16.622124	19
TAACGGC	945	0.0	16.529003	36
ATCACGG	280	0.0	16.499903	35
>>END_MODULE