FastQCFastQC Report
Thu 2 Feb 2017
SRR4062453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850832
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG57640.3114275093579536No Hit
TCCATGTACTCTGCGTTGATACCAC48360.26128789647034417No Hit
GTATCAACGCAGAGTACTTTTTTTT32220.17408387146969578No Hit
GAGTACATGGAAGCAGTGGTATCAA23760.1283746985139656No Hit
TATCAACGCAGAGTACTTTTTTTTT23680.12794246047183105No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21730.11740665819480105No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21190.11448905141039274No Hit
CATGTACTCTGCGTTGATACCACTG20630.11146338511545079No Hit
GAATAGGACCGCGGTTCTATTTTGT19900.10751921298097288No Hit
GCGCAAGACGGACCAGAGCGAAAGC19840.10719503444937195No Hit
GAATAACGCCGCCGCATCGCCAGTC18970.10249444574115857No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18830.10173802916742308No Hit
GATTAAGAGGGACGGCCGGGGGCAT18800.10157593990162263No Hit
GTATCTGATCGTCTTCGAACCTCCG18700.10103564234895443No Hit
GTCCTATTCCATTATTCCTAGCTGC18680.10092758283842078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1900.013.989721
GCATCGC3400.013.41279614
ATCGCCA3550.013.38275816
CGCATCG3500.013.30102313
TCTATAC1301.4551915E-1113.1488093
CGACCAT2400.013.06385710
AACGGAC1352.7284841E-1112.66832615
TTCCGTT604.088158E-412.66832515
CGTTATT1950.012.6590782
CGCCAGT3950.012.50932218
TCGCCAG3800.012.50265217
CATCGCC3800.012.50163715
CCGACCA2600.012.0589469
GACCATA2700.011.96420711
GAACGTC1800.011.6107475
CGAACGA1406.7848305E-1011.5378516
CGGTCCA4200.011.31069910
CGCAAGA4200.011.3027482
TCTTATA1604.5474735E-1111.2744912
CCGCATC4300.011.26861412
Produced by FastQC (version 0.11.5)