##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062453_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1850832
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15757886183079	32.0	32.0	32.0	32.0	32.0
2	30.745700312075865	32.0	32.0	32.0	32.0	32.0
3	30.729423308004183	32.0	32.0	32.0	32.0	32.0
4	30.822481456987994	32.0	32.0	32.0	32.0	32.0
5	30.570513693301177	32.0	32.0	32.0	32.0	32.0
6	34.22963078226441	36.0	36.0	36.0	32.0	36.0
7	34.10984357305255	36.0	36.0	36.0	32.0	36.0
8	34.09695477493365	36.0	36.0	36.0	32.0	36.0
9	34.299700891274846	36.0	36.0	36.0	32.0	36.0
10	33.89734778737346	36.0	36.0	36.0	32.0	36.0
11	34.28291762839631	36.0	36.0	36.0	32.0	36.0
12	34.010238638623065	36.0	36.0	36.0	32.0	36.0
13	34.15593959905599	36.0	36.0	36.0	32.0	36.0
14	34.05115591258418	36.0	36.0	36.0	32.0	36.0
15	33.97386094469947	36.0	36.0	36.0	32.0	36.0
16	33.978825738910935	36.0	36.0	36.0	32.0	36.0
17	33.89143477095706	36.0	36.0	36.0	32.0	36.0
18	33.9032786336091	36.0	36.0	36.0	32.0	36.0
19	33.90106449423827	36.0	36.0	36.0	32.0	36.0
20	33.89124458621852	36.0	36.0	36.0	32.0	36.0
21	33.87241143442517	36.0	36.0	36.0	32.0	36.0
22	33.80997681042904	36.0	36.0	36.0	32.0	36.0
23	33.76782765804784	36.0	36.0	36.0	32.0	36.0
24	33.725358649515464	36.0	36.0	36.0	27.0	36.0
25	33.26556273070705	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	69.0
5	178.0
6	323.0
7	75.0
8	278.0
9	333.0
10	220.0
11	70.0
12	152.0
13	115.0
14	291.0
15	337.0
16	555.0
17	744.0
18	1043.0
19	1573.0
20	2372.0
21	3627.0
22	5537.0
23	8707.0
24	12256.0
25	17333.0
26	24230.0
27	31033.0
28	42120.0
29	55877.0
30	71320.0
31	97607.0
32	138155.0
33	197014.0
34	420465.0
35	716810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.90827401298762	17.002452063721236	11.081134238871423	26.008139684419724
2	17.012134929567267	19.635471232830824	36.53889482934968	26.81349900825223
3	19.142654597954547	23.447374449948562	28.204941169351006	29.20502978274589
4	12.703008530492212	15.036654205425664	35.29973445560156	36.96060280848056
5	14.86956940038624	36.276154087715476	33.36102491647934	15.493251595418943
6	35.033452635209244	34.641978151593925	16.617989122825794	13.706580090371038
7	30.67508445178787	30.2134651401186	20.634026393076034	18.477424015017498
8	28.67686503884957	32.36270701022722	19.410615709415612	19.549812241507606
9	27.285623504279283	14.306296561701437	18.358107651329757	40.04997228268953
10	15.818191948528398	26.596979061803033	31.53068405755394	26.05414493211463
11	37.78194797270103	20.999011126450313	21.68153944025862	19.537501460590036
12	24.566612528173838	23.631830836867124	28.492068043537305	23.309488591421736
13	29.586947642888635	19.148740502716986	25.129520084606625	26.134791769787753
14	23.73316887655463	19.485585687932446	24.68745097402773	32.093794461485196
15	25.507530947338815	26.76170616729008	22.360409043086918	25.370353842284192
16	26.3056743615444	25.524268425866776	23.47317250485336	24.696884707735464
17	24.382047514665757	25.64819282844042	25.165916157972525	24.803843498921303
18	25.452848664527895	24.345518580834337	26.158428054381872	24.043204700255895
19	25.501181845084968	25.49734064567857	24.93896009534831	24.062517413888155
20	25.46722247947618	24.0769150874399	25.281512768692142	25.17434966439177
21	26.737559415416147	24.31268549565955	24.061444710256186	24.888310378668123
22	25.56755186088831	24.43231286277731	24.68555016073534	25.314585115599037
23	24.19851210604626	24.21138915190045	25.14843771134857	26.441661030704722
24	24.649727946399732	25.283969639932717	24.825939257142455	25.240363156525092
25	25.184930309960134	24.323373903578176	24.787234790756756	25.70446099570493
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	190.0
1	190.0
2	519.5
3	849.0
4	849.0
5	849.0
6	2208.0
7	3567.0
8	3567.0
9	3567.0
10	3846.0
11	4125.0
12	4125.0
13	4125.0
14	4681.5
15	5238.0
16	5238.0
17	5238.0
18	8452.5
19	11667.0
20	11667.0
21	11667.0
22	18635.5
23	25604.0
24	25604.0
25	25604.0
26	37825.0
27	50046.0
28	50046.0
29	50046.0
30	60725.0
31	71404.0
32	71404.0
33	71404.0
34	90872.0
35	110340.0
36	110340.0
37	110340.0
38	126286.0
39	142232.0
40	142232.0
41	142232.0
42	164421.5
43	186611.0
44	186611.0
45	186611.0
46	221269.0
47	255927.0
48	255927.0
49	255927.0
50	252432.5
51	248938.0
52	248938.0
53	248938.0
54	231444.5
55	213951.0
56	213951.0
57	213951.0
58	197962.0
59	181973.0
60	181973.0
61	181973.0
62	160959.0
63	139945.0
64	139945.0
65	139945.0
66	116576.5
67	93208.0
68	93208.0
69	93208.0
70	70440.0
71	47672.0
72	47672.0
73	47672.0
74	37355.0
75	27038.0
76	27038.0
77	27038.0
78	22323.0
79	17608.0
80	17608.0
81	17608.0
82	12386.0
83	7164.0
84	7164.0
85	7164.0
86	5242.0
87	3320.0
88	3320.0
89	3320.0
90	2248.0
91	1176.0
92	1176.0
93	1176.0
94	708.5
95	241.0
96	241.0
97	241.0
98	519.5
99	798.0
100	798.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007888344268955799
2	0.003187755560742412
3	0.005186856505614772
4	0.01588474804844524
5	0.037334560889372995
6	0.048788869005938956
7	0.0678613726151266
8	0.08904103667972026
9	0.09763176776714473
10	0.11891949134227202
11	0.12232336592408172
12	0.13431797159331588
13	0.12302575274255037
14	0.1254570917295573
15	0.1149212894525273
16	0.11416487287879182
17	0.12470067515582181
18	0.13086006725623936
19	0.13242693015897714
20	0.12145888983981258
21	0.12999559117197024
22	0.14928421380222517
23	0.13950482809893064
24	0.1341558823275154
25	0.13145439456417438
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1850832.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.16665674382348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91717745936639	42.4892914191736
2	12.256726882018851	13.032983818781805
3	3.4558969327730367	5.512154579003294
4	1.4542548351949909	3.092714705634307
5	0.7373368569975164	1.9600867790278302
6	0.46768634737756976	1.4919191696877503
7	0.3096700999306322	1.1524886734786208
8	0.222797840326829	0.9476333039937369
9	0.1592212586837661	0.7618735806083385
>10	0.8428557430423743	8.073140383740526
>50	0.07848218542512407	2.9339608579353706
>100	0.08025297163821365	9.344427540884995
>500	0.012032308049280788	4.357398427824068
>1k	0.0055063104632301916	4.53744098059301
>5k	1.0196871228204058E-4	0.31248577963269586
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5764	0.3114275093579536	No Hit
TCCATGTACTCTGCGTTGATACCAC	4836	0.26128789647034417	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3222	0.17408387146969578	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2376	0.1283746985139656	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2368	0.12794246047183105	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2173	0.11740665819480105	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2119	0.11448905141039274	No Hit
CATGTACTCTGCGTTGATACCACTG	2063	0.11146338511545079	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1990	0.10751921298097288	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1984	0.10719503444937195	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1897	0.10249444574115857	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1883	0.10173802916742308	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1880	0.10157593990162263	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1870	0.10103564234895443	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1868	0.10092758283842078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0805951053364108E-4
2	0.0	0.0	0.0	0.0	1.0805951053364108E-4
3	0.0	0.0	0.0	0.0	1.0805951053364108E-4
4	0.0	0.0	0.0	0.0	1.0805951053364108E-4
5	0.0	0.0	0.0	5.402975526682054E-5	1.0805951053364108E-4
6	0.0	0.0	0.0	5.402975526682054E-5	1.6208926580046164E-4
7	0.0	0.0	0.0	5.402975526682054E-5	1.6208926580046164E-4
8	0.0	0.0	0.0	5.402975526682054E-5	1.6208926580046164E-4
9	0.0	0.0	0.0	5.402975526682054E-5	1.6208926580046164E-4
10	0.0	0.0	0.0	5.402975526682054E-5	1.6208926580046164E-4
11	0.0	0.0	0.0	1.0805951053364108E-4	1.6208926580046164E-4
12	0.0	0.0	0.0	2.1611902106728216E-4	4.3223804213456433E-4
13	0.0	0.0	0.0	2.1611902106728216E-4	4.862677974013849E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	190	0.0	13.98972	1
GCATCGC	340	0.0	13.412796	14
ATCGCCA	355	0.0	13.382758	16
CGCATCG	350	0.0	13.301023	13
TCTATAC	130	1.4551915E-11	13.148809	3
CGACCAT	240	0.0	13.063857	10
AACGGAC	135	2.7284841E-11	12.668326	15
TTCCGTT	60	4.088158E-4	12.668325	15
CGTTATT	195	0.0	12.659078	2
CGCCAGT	395	0.0	12.509322	18
TCGCCAG	380	0.0	12.502652	17
CATCGCC	380	0.0	12.501637	15
CCGACCA	260	0.0	12.058946	9
GACCATA	270	0.0	11.964207	11
GAACGTC	180	0.0	11.610747	5
CGAACGA	140	6.7848305E-10	11.53785	16
CGGTCCA	420	0.0	11.310699	10
CGCAAGA	420	0.0	11.302748	2
TCTTATA	160	4.5474735E-11	11.274491	2
CCGCATC	430	0.0	11.268614	12
>>END_MODULE