FastQCFastQC Report
Thu 2 Feb 2017
SRR4062453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850832
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA25440.13745169739879148No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21880.11821710452380335No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21790.11773083672640197No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20330.10984249245744616No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20280.10957234368111206No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19620.10600637983350192No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18560.10027922577521893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG4800.020.6249128
ATACCGT5100.020.2744246
TACCGTC4950.019.5554717
TAATACC2600.018.6153054
CGTCGTA5400.018.33325410
CCGTCGT5800.017.0688929
GTATTAG5950.017.0110871
CGCAATA6300.016.76183336
TTAATAC2500.016.7199293
TACGAAT6450.016.71310841
ATACGAA6450.016.71310840
CAATACG6450.016.71310838
CGTCTTA2900.016.68958315
GATATAC1753.621608E-916.3454361
GTCGTAG6100.016.22943911
CGAGCCG7750.016.18057615
AGATACC7000.016.0285034
CTAGGAC3200.015.8124323
GACGGTA6450.015.6898568
CTAGCGG7300.015.67116529
Produced by FastQC (version 0.11.5)