##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062453_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1850832
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298890985243393	32.0	32.0	32.0	32.0	32.0
2	31.417312862539656	32.0	32.0	32.0	32.0	32.0
3	31.50226546763834	32.0	32.0	32.0	32.0	32.0
4	31.60002636652057	32.0	32.0	32.0	32.0	32.0
5	31.536278279173906	32.0	32.0	32.0	32.0	32.0
6	35.130160922223084	36.0	36.0	36.0	36.0	36.0
7	35.149191282623164	36.0	36.0	36.0	36.0	36.0
8	35.0891188395273	36.0	36.0	36.0	36.0	36.0
9	35.225414840460935	36.0	36.0	36.0	36.0	36.0
10	35.09820286228032	36.0	36.0	36.0	36.0	36.0
11	35.23117441237238	36.0	36.0	36.0	36.0	36.0
12	35.13583998979918	36.0	36.0	36.0	36.0	36.0
13	35.183379690863354	36.0	36.0	36.0	36.0	36.0
14	35.13914931230927	36.0	36.0	36.0	36.0	36.0
15	35.102366395221175	36.0	36.0	36.0	36.0	36.0
16	35.10811408058646	36.0	36.0	36.0	36.0	36.0
17	35.084452829862464	36.0	36.0	36.0	36.0	36.0
18	35.098722628525984	36.0	36.0	36.0	36.0	36.0
19	35.08059780682417	36.0	36.0	36.0	36.0	36.0
20	35.073880827649404	36.0	36.0	36.0	36.0	36.0
21	35.06225524520864	36.0	36.0	36.0	36.0	36.0
22	35.04342371430794	36.0	36.0	36.0	36.0	36.0
23	34.99825754039264	36.0	36.0	36.0	36.0	36.0
24	34.9726436543133	36.0	36.0	36.0	36.0	36.0
25	34.94812116929035	36.0	36.0	36.0	32.0	36.0
26	34.89889141748144	36.0	36.0	36.0	32.0	36.0
27	34.88269005506713	36.0	36.0	36.0	32.0	36.0
28	34.8658370938043	36.0	36.0	36.0	32.0	36.0
29	34.837677325656784	36.0	36.0	36.0	32.0	36.0
30	34.82153377508061	36.0	36.0	36.0	32.0	36.0
31	34.820961599972335	36.0	36.0	36.0	32.0	36.0
32	34.78527656751126	36.0	36.0	36.0	32.0	36.0
33	34.76175093147298	36.0	36.0	36.0	32.0	36.0
34	34.74848878774519	36.0	36.0	36.0	32.0	36.0
35	34.7086202313338	36.0	36.0	36.0	32.0	36.0
36	34.68894961833381	36.0	36.0	36.0	32.0	36.0
37	34.664301243981086	36.0	36.0	36.0	32.0	36.0
38	34.62669869550559	36.0	36.0	36.0	32.0	36.0
39	34.59465797003726	36.0	36.0	36.0	32.0	36.0
40	34.567892709873185	36.0	36.0	36.0	32.0	36.0
41	34.54450376911573	36.0	36.0	36.0	32.0	36.0
42	34.487552084684076	36.0	36.0	36.0	32.0	36.0
43	34.500540837850224	36.0	36.0	36.0	32.0	36.0
44	34.468694079203296	36.0	36.0	36.0	32.0	36.0
45	34.40107043751134	36.0	36.0	36.0	32.0	36.0
46	34.4327615904631	36.0	36.0	36.0	32.0	36.0
47	34.389081775115194	36.0	36.0	36.0	32.0	36.0
48	34.365838714696956	36.0	36.0	36.0	32.0	36.0
49	34.37323430759788	36.0	36.0	36.0	32.0	36.0
50	33.83875143719149	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	40.0
22	151.0
23	428.0
24	1178.0
25	2946.0
26	6066.0
27	11521.0
28	19321.0
29	29463.0
30	42074.0
31	60557.0
32	90275.0
33	143611.0
34	318846.0
35	1124345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.507291134725904	17.52720159305282	11.567411586379075	26.398095685842204
2	16.320040113619232	20.05399993408053	36.25127854056452	27.37468141173572
3	18.451005118795607	23.68564089763165	28.54460657025566	29.31874741331708
4	12.371395258117186	15.511722874003283	35.54250912835674	36.574372739522786
5	14.618182525480433	36.35824321170155	33.40967737752535	15.61389688529267
6	35.25176124541487	34.86636471057979	16.34474646656808	13.537127577437255
7	30.605603645065376	30.264189437069078	20.61949470264978	18.51071221521576
8	28.673320971325328	32.57491765865297	19.245560915307276	19.50620045471442
9	27.30269413971663	14.10214433292703	18.345641311583115	40.24952021577323
10	15.763775426402828	26.557353665810833	31.607352801334756	26.071518106451585
11	37.93488550014264	20.864562531877556	21.676197515495733	19.524354452484072
12	24.488770455665342	23.525744097789534	28.553266855122455	23.432218591422668
13	29.764830087225636	19.00912670625967	25.15285017764983	26.073193028864853
14	23.63661315559705	19.360968472557207	24.699000233408544	32.3034181384372
15	25.61021205598347	26.66503496805761	22.303158795611917	25.421594180347
16	26.420064057677845	25.369401436759254	23.452317660381926	24.75821684518098
17	24.496064472626365	25.64614184323591	25.10006310675415	24.757730577383576
18	25.59951416444064	24.24941863983333	26.055039031095205	24.096028164630827
19	25.621288155813172	25.341468053286302	24.97298512236659	24.064258668533935
20	25.580009422789317	23.91497445473171	25.299865141730855	25.20515098074812
21	26.813995003328234	24.295776169852264	23.975001512833146	24.915227313986357
22	25.63506574340621	24.364069780509524	24.6954882993162	25.305376176768068
23	24.355641138687897	23.96803167440373	25.18321490010979	26.49311228679859
24	24.72131452233374	25.17332745489596	24.80895078537652	25.296407237393776
25	25.301540064144124	24.237370004408827	24.725312724223482	25.735777207223563
26	24.739738668879728	24.870598736135964	25.311265420092155	25.07839717489216
27	25.797046949696135	24.676145646930678	24.479423308004183	25.047384095369
28	24.855308315395455	24.121854387648366	25.3996040699534	25.62323322700277
29	24.688734579907848	24.845636989202692	25.462818883615583	25.002809547273873
30	25.04192709008705	24.670472522627662	25.569041382470154	24.718559004815134
31	25.115785765536796	24.611580089386827	24.440197705680472	25.832436439395906
32	24.803493780094573	24.727527944189422	24.59250758577764	25.876470689938362
33	24.581161337171604	24.240935968256437	25.093849684898466	26.084053009673486
34	25.107411153470437	24.460837072192398	25.571256602436094	24.86049517190107
35	26.1364618722823	24.203979615653935	25.10497981448343	24.554578697580332
36	24.865357849875082	24.96158484400529	24.778369943895502	25.394687362224122
37	25.908456305056323	24.637406312404366	24.720720195025805	24.733417187513506
38	24.80046811379963	24.368122012154533	25.102386386230624	25.729023487815212
39	25.266204604199626	24.439171140330405	24.68035996784149	25.614264287628487
40	25.66364748394236	24.529670980402326	25.050733940195546	24.75594759545977
41	24.45176007330757	24.87362440243091	25.53024801818858	25.144367506072946
42	26.11376937507024	24.947320988614848	24.78312456235898	24.15578507395593
43	25.102656535006957	23.886176595174494	25.012264754445567	25.998902115372978
44	24.841368638536615	24.526699343862653	25.103791159867562	25.528140857733174
45	24.966987819531973	24.763079523154993	25.01950474165132	25.25042791566171
46	24.627086629148405	24.433930254069523	25.438721612766585	25.50026150401549
47	24.926951770879256	24.164213715777553	25.723674542043796	25.18515997129939
48	25.553318723687507	25.401278992366677	24.365528583901728	24.679873700044087
49	24.406645227659777	24.891562281179493	25.271499520215773	25.430292970944958
50	24.371363797470543	25.56774466834375	24.570247326607706	25.490644207577994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	18.0
2	29.0
3	81.0
4	133.0
5	176.5
6	220.0
7	202.5
8	185.0
9	279.0
10	373.0
11	543.5
12	714.0
13	1293.0
14	1872.0
15	2529.0
16	3186.0
17	3897.5
18	4609.0
19	4976.5
20	5344.0
21	6324.0
22	7304.0
23	7935.0
24	8566.0
25	9975.0
26	11384.0
27	14081.5
28	16779.0
29	19234.5
30	21690.0
31	23667.5
32	25645.0
33	29758.5
34	33872.0
35	39209.5
36	44547.0
37	54208.0
38	63869.0
39	69905.5
40	75942.0
41	84317.0
42	92692.0
43	96195.0
44	99698.0
45	112575.0
46	125452.0
47	135520.5
48	145589.0
49	147749.5
50	149910.0
51	141623.5
52	133337.0
53	131170.0
54	129003.0
55	130712.5
56	132422.0
57	128576.0
58	124730.0
59	114659.0
60	104588.0
61	92278.0
62	79968.0
63	69691.5
64	59415.0
65	50932.0
66	42449.0
67	37083.0
68	31717.0
69	29349.5
70	26982.0
71	21105.5
72	15229.0
73	13921.5
74	12614.0
75	9193.5
76	5773.0
77	5278.0
78	4783.0
79	4071.5
80	3360.0
81	2670.5
82	1981.0
83	1699.5
84	1418.0
85	1154.5
86	891.0
87	648.0
88	405.0
89	254.0
90	103.0
91	63.5
92	24.0
93	18.5
94	13.0
95	9.0
96	5.0
97	6.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0160468373142457
2	0.004808648218747029
3	3.241785316009233E-4
4	3.241785316009233E-4
5	0.0
6	5.94327307935026E-4
7	5.402975526682054E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1850832.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.951639102562346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.28546362732585	44.921032860580326
2	12.197343243477638	13.649226943382759
3	3.353605818082212	5.629192272767678
4	1.3630806806878213	3.0506639325408
5	0.7357082881320431	2.0582042311164015
6	0.4643429756546507	1.5588450356183343
7	0.2998561154174143	1.174420880677201
8	0.20930017037634704	0.9368550077601743
9	0.15927783728712297	0.8020670462033176
>10	0.777816036849108	7.847429791957912
>50	0.06805925852077872	2.703818296289656
>100	0.0718701079557652	8.661463334685978
>500	0.01077971609447932	4.1022403299202015
>1k	0.0034961241387500494	2.904540036499344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2544	0.13745169739879148	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2188	0.11821710452380335	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2179	0.11773083672640197	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2033	0.10984249245744616	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2028	0.10957234368111206	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1962	0.10600637983350192	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1856	0.10027922577521893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.402975526682054E-5
2	0.0	0.0	0.0	0.0	5.402975526682054E-5
3	0.0	0.0	0.0	0.0	5.402975526682054E-5
4	0.0	0.0	0.0	0.0	5.402975526682054E-5
5	0.0	0.0	0.0	5.402975526682054E-5	5.402975526682054E-5
6	0.0	0.0	0.0	5.402975526682054E-5	5.402975526682054E-5
7	0.0	0.0	0.0	5.402975526682054E-5	5.402975526682054E-5
8	0.0	0.0	0.0	5.402975526682054E-5	5.402975526682054E-5
9	0.0	0.0	0.0	1.0805951053364108E-4	5.402975526682054E-5
10	0.0	0.0	0.0	1.0805951053364108E-4	5.402975526682054E-5
11	0.0	0.0	0.0	2.1611902106728216E-4	5.402975526682054E-5
12	0.0	0.0	0.0	2.701487763341027E-4	2.701487763341027E-4
13	0.0	0.0	0.0	2.701487763341027E-4	3.782082868677438E-4
14	0.0	0.0	0.0	2.701487763341027E-4	3.782082868677438E-4
15	0.0	0.0	0.0	3.782082868677438E-4	5.402975526682054E-4
16	0.0	0.0	0.0	4.3223804213456433E-4	5.94327307935026E-4
17	0.0	0.0	0.0	4.3223804213456433E-4	5.94327307935026E-4
18	0.0	0.0	0.0	4.862677974013849E-4	6.483570632018465E-4
19	0.0	0.0	0.0	5.94327307935026E-4	6.483570632018465E-4
20	0.0	0.0	0.0	8.644760842691287E-4	7.564165737354876E-4
21	0.0	0.0	0.0	0.0010265653500695904	7.564165737354876E-4
22	0.0	0.0	0.0	0.001296714126403693	7.564165737354876E-4
23	0.0	0.0	0.0	0.001891041434338719	8.104463290023082E-4
24	0.0	0.0	0.0	0.003079696050208771	8.104463290023082E-4
25	0.0	0.0	0.0	0.0039982018897447204	8.104463290023082E-4
26	0.0	0.0	0.0	0.0052408862608815925	8.104463290023082E-4
27	0.0	0.0	0.0	0.006753719408352568	8.104463290023082E-4
28	0.0	0.0	0.0	0.010751921298097288	8.104463290023082E-4
29	0.0	0.0	0.0	0.019936979693456782	8.104463290023082E-4
30	0.0	0.0	0.0	0.03479516239183243	8.104463290023082E-4
31	0.0	0.0	0.0	0.06905002723099665	8.644760842691287E-4
32	0.0	0.0	0.0	0.10989652221271298	8.644760842691287E-4
33	0.0	0.0	0.0	0.15268808838403486	8.644760842691287E-4
34	0.0	0.0	0.0	0.20412441539804801	8.644760842691287E-4
35	0.0	0.0	0.0	0.26101774769401004	8.644760842691287E-4
36	0.0	0.0	0.0	0.3444937195812478	8.644760842691287E-4
37	0.0	0.0	0.0	0.47070722788454056	8.644760842691287E-4
38	0.0	0.0	0.0	0.63555201120361	9.185058395359492E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	480	0.0	20.624912	8
ATACCGT	510	0.0	20.274424	6
TACCGTC	495	0.0	19.555471	7
TAATACC	260	0.0	18.615305	4
CGTCGTA	540	0.0	18.333254	10
CCGTCGT	580	0.0	17.068892	9
GTATTAG	595	0.0	17.011087	1
CGCAATA	630	0.0	16.761833	36
TTAATAC	250	0.0	16.719929	3
TACGAAT	645	0.0	16.713108	41
ATACGAA	645	0.0	16.713108	40
CAATACG	645	0.0	16.713108	38
CGTCTTA	290	0.0	16.689583	15
GATATAC	175	3.621608E-9	16.345436	1
GTCGTAG	610	0.0	16.229439	11
CGAGCCG	775	0.0	16.180576	15
AGATACC	700	0.0	16.028503	4
CTAGGAC	320	0.0	15.812432	3
GACGGTA	645	0.0	15.689856	8
CTAGCGG	730	0.0	15.671165	29
>>END_MODULE