FastQCFastQC Report
Thu 2 Feb 2017
SRR4062452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877213
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49470.2635289655462646No Hit
TATCAACGCAGAGTACTTTTTTTTT33730.1796812615297252No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32580.17355515863143928No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23690.12619771970468988No Hit
GAATAACGCCGCCGCATCGCCAGTC23440.1248659582050625No Hit
GATTAAGAGGGACGGCCGGGGGCAT23270.12396036038531588No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21730.11575670954761126No Hit
GCGCAAGACGGACCAGAGCGAAAGC21520.11463802988792428No Hit
GAATAGGACCGCGGTTCTATTTTGT21490.11447821850796899No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21440.11421186620804351No Hit
GTATCTGATCGTCTTCGAACCTCCG20240.10781941100983213No Hit
GAACTACGACGGTATCTGATCGTCT20160.10739324732995136No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20000.10654091997018984No Hit
CTATTGGAGCTGGAATTACCGCGGC19710.10499607663062209No Hit
GTCCTATTCCATTATTCCTAGCTGC19660.10472972433069662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCA3550.015.52581216
CGCATCG3850.013.81903513
CCGTCGT3600.013.1956119
CGCCAGT4200.012.89743718
GAGTACG604.1173602E-412.6576591
GCATCGC4150.012.59147514
CGTCGTA3700.012.58219410
GTCGTAG3650.012.49292311
CGGTCCA4200.012.44157710
TCGCCAG4450.012.3860917
AACGGAC1251.4078978E-912.16204815
AGCAACT2950.011.9159913
CGAACGA1850.011.81437716
CATCGCC4550.011.69427715
TATGACC1302.6375346E-911.6861733
ACCGTCG4000.011.638848
TACCGTC4100.011.58669957
CGGACAT2050.011.5848465
GCAACTT3050.011.52560914
CCGCATC4650.011.44156712
Produced by FastQC (version 0.11.5)