##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062452_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1877213
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.163846617299157	32.0	32.0	32.0	32.0	32.0
2	30.86968553914766	32.0	32.0	32.0	32.0	32.0
3	30.871926627399233	32.0	32.0	32.0	32.0	32.0
4	30.91761190658705	32.0	32.0	32.0	32.0	32.0
5	30.77440386359992	32.0	32.0	32.0	32.0	32.0
6	34.392682663075526	36.0	36.0	36.0	32.0	36.0
7	34.271459338924245	36.0	36.0	36.0	32.0	36.0
8	34.275471137265725	36.0	36.0	36.0	32.0	36.0
9	34.3938673981056	36.0	36.0	36.0	32.0	36.0
10	34.12946160078798	36.0	36.0	36.0	32.0	36.0
11	34.39910974407273	36.0	36.0	36.0	32.0	36.0
12	34.2040732724523	36.0	36.0	36.0	32.0	36.0
13	34.3202183236532	36.0	36.0	36.0	32.0	36.0
14	34.22958715926216	36.0	36.0	36.0	32.0	36.0
15	34.1872675077362	36.0	36.0	36.0	32.0	36.0
16	34.18597782989996	36.0	36.0	36.0	32.0	36.0
17	34.106834972909304	36.0	36.0	36.0	32.0	36.0
18	34.12674747085173	36.0	36.0	36.0	32.0	36.0
19	34.11015798420318	36.0	36.0	36.0	32.0	36.0
20	34.09321158547272	36.0	36.0	36.0	32.0	36.0
21	34.064071578451674	36.0	36.0	36.0	32.0	36.0
22	34.01730703974456	36.0	36.0	36.0	32.0	36.0
23	34.01438089337758	36.0	36.0	36.0	32.0	36.0
24	33.98055361858244	36.0	36.0	36.0	32.0	36.0
25	33.535801211689886	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	59.0
5	179.0
6	323.0
7	73.0
8	297.0
9	323.0
10	198.0
11	66.0
12	145.0
13	130.0
14	369.0
15	463.0
16	825.0
17	1062.0
18	1425.0
19	2038.0
20	2940.0
21	3966.0
22	5889.0
23	8283.0
24	11330.0
25	15490.0
26	21217.0
27	27090.0
28	36414.0
29	48335.0
30	62186.0
31	85138.0
32	122527.0
33	180445.0
34	418135.0
35	819841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12029578485275	17.8484741934109	11.107168733751012	25.92406128798534
2	17.199749404943283	20.393078377013442	36.158298936888826	26.248873281154445
3	18.641776160176608	24.43488137196824	28.49960151891613	28.42374094893902
4	12.680618726860743	15.461938004774764	35.98570234385448	35.871740924510014
5	14.935792292943601	36.31622868580162	33.090922461560105	15.657056559694677
6	33.67614008515364	35.52763762855253	17.070539812713147	13.72568247358068
7	30.008283794041642	30.628608834341176	21.051391506818938	18.31171586479825
8	28.501973220502723	31.639772362543678	19.920623326548	19.937631090405596
9	27.796197159066672	14.049503049950946	18.992663055069745	39.161636735912644
10	16.376674936906532	26.213335921783298	30.97297274234185	26.437016398968318
11	37.284125630469696	21.36916923867901	22.189066507519236	19.15763862333206
12	24.810628790824623	23.994523023517182	28.247660896852235	22.947187288805964
13	29.113526411593444	19.468573449152558	25.539162587980424	25.878737551273577
14	23.805457565248744	19.847810712239895	24.960877955221125	31.38585376729024
15	25.539125783483605	26.701558674409466	22.428869847726997	25.330445694379932
16	25.932704783876794	25.71186452428683	23.482929810555213	24.872500881281162
17	23.974569920717677	25.514988439660897	25.122656931269983	25.387784708351447
18	24.956421925372148	24.510720099680178	26.22247967245503	24.310378302492644
19	25.61676256394856	25.010974802493564	25.40350734951719	23.96875528404069
20	25.685739383566574	24.112628250291877	24.93467815356914	25.266954212572408
21	27.138602945294586	23.92959840237851	24.34089845939832	24.590900192928583
22	25.853959643317864	24.267733540476723	24.70894468908752	25.169362127117896
23	24.50941900572598	24.030547088627312	25.454200171341938	26.00583373430477
24	24.6977524463849	25.120058889265245	25.11099079588522	25.07119786846464
25	24.78161076496107	24.469568992969194	25.278317257403526	25.470502984666215
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	297.0
1	297.0
2	782.5
3	1268.0
4	1268.0
5	1268.0
6	3398.0
7	5528.0
8	5528.0
9	5528.0
10	5691.0
11	5854.0
12	5854.0
13	5854.0
14	5995.5
15	6137.0
16	6137.0
17	6137.0
18	10038.5
19	13940.0
20	13940.0
21	13940.0
22	21561.5
23	29183.0
24	29183.0
25	29183.0
26	43379.5
27	57576.0
28	57576.0
29	57576.0
30	69017.5
31	80459.0
32	80459.0
33	80459.0
34	99787.5
35	119116.0
36	119116.0
37	119116.0
38	133434.0
39	147752.0
40	147752.0
41	147752.0
42	167768.5
43	187785.0
44	187785.0
45	187785.0
46	214498.0
47	241211.0
48	241211.0
49	241211.0
50	243529.0
51	245847.0
52	245847.0
53	245847.0
54	227273.5
55	208700.0
56	208700.0
57	208700.0
58	191127.0
59	173554.0
60	173554.0
61	173554.0
62	156729.0
63	139904.0
64	139904.0
65	139904.0
66	117360.5
67	94817.0
68	94817.0
69	94817.0
70	72581.5
71	50346.0
72	50346.0
73	50346.0
74	40655.0
75	30964.0
76	30964.0
77	30964.0
78	25859.5
79	20755.0
80	20755.0
81	20755.0
82	14846.5
83	8938.0
84	8938.0
85	8938.0
86	6736.5
87	4535.0
88	4535.0
89	4535.0
90	3076.0
91	1617.0
92	1617.0
93	1617.0
94	978.5
95	340.0
96	340.0
97	340.0
98	565.0
99	790.0
100	790.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009215789577421422
2	0.004314907258792689
3	0.004527989098733068
4	0.013956860516094871
5	0.03446598761035642
6	0.04474718638747974
7	0.06717405004120469
8	0.08517946551616679
9	0.0944485255535733
10	0.11810060978695545
11	0.11836696208688093
12	0.13083224972339313
13	0.11836696208688093
14	0.12310803302555437
15	0.11154834320878879
16	0.11197450688866953
17	0.12081740324619529
18	0.12875470178397444
19	0.1312584134032739
20	0.1207108623262251
21	0.12923413592384028
22	0.15059559037786335
23	0.1384499255012617
24	0.13360231364261807
25	0.1312584134032739
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1877213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.60456022748298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.38340257706452	35.59814885782882
2	13.85673815819532	12.915743761001504
3	4.270048651690188	5.970112186859335
4	1.7984953121028682	3.352723323669757
5	0.9050260116404785	2.10891696334687
6	0.5620714487117775	1.5717055610181985
7	0.3732914469090911	1.2177958603915302
8	0.26980046747067815	1.0059145708512227
9	0.20071425029531031	0.8418779429761695
>10	1.1299231169402593	9.749169926870756
>50	0.1139613884807326	3.746814406186596
>100	0.11209050508562683	11.359459677964107
>500	0.01789727607722704	5.637731496030907
>1k	0.00653938933590988	4.92388546500429
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4947	0.2635289655462646	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3373	0.1796812615297252	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3258	0.17355515863143928	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2369	0.12619771970468988	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2344	0.1248659582050625	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2327	0.12396036038531588	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2173	0.11575670954761126	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2152	0.11463802988792428	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2149	0.11447821850796899	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2144	0.11421186620804351	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2024	0.10781941100983213	No Hit
GAACTACGACGGTATCTGATCGTCT	2016	0.10739324732995136	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2000	0.10654091997018984	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1971	0.10499607663062209	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1966	0.10472972433069662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0654091997018985E-4
2	0.0	0.0	0.0	0.0	1.0654091997018985E-4
3	0.0	0.0	0.0	0.0	1.0654091997018985E-4
4	0.0	0.0	0.0	0.0	1.0654091997018985E-4
5	0.0	0.0	0.0	0.0	1.5981137995528478E-4
6	0.0	0.0	0.0	0.0	3.1962275991056957E-4
7	0.0	0.0	0.0	0.0	3.7289321989566446E-4
8	0.0	0.0	0.0	0.0	3.7289321989566446E-4
9	0.0	0.0	0.0	0.0	3.7289321989566446E-4
10	0.0	0.0	0.0	0.0	3.7289321989566446E-4
11	0.0	0.0	0.0	0.0	3.7289321989566446E-4
12	0.0	0.0	0.0	1.0654091997018985E-4	3.7289321989566446E-4
13	0.0	0.0	0.0	1.0654091997018985E-4	4.7943413986585435E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCCA	355	0.0	15.525812	16
CGCATCG	385	0.0	13.819035	13
CCGTCGT	360	0.0	13.195611	9
CGCCAGT	420	0.0	12.897437	18
GAGTACG	60	4.1173602E-4	12.657659	1
GCATCGC	415	0.0	12.591475	14
CGTCGTA	370	0.0	12.582194	10
GTCGTAG	365	0.0	12.492923	11
CGGTCCA	420	0.0	12.441577	10
TCGCCAG	445	0.0	12.38609	17
AACGGAC	125	1.4078978E-9	12.162048	15
AGCAACT	295	0.0	11.91599	13
CGAACGA	185	0.0	11.814377	16
CATCGCC	455	0.0	11.694277	15
TATGACC	130	2.6375346E-9	11.686173	3
ACCGTCG	400	0.0	11.63884	8
TACCGTC	410	0.0	11.5866995	7
CGGACAT	205	0.0	11.584846	5
GCAACTT	305	0.0	11.525609	14
CCGCATC	465	0.0	11.441567	12
>>END_MODULE