FastQCFastQC Report
Thu 2 Feb 2017
SRR4062452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877213
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24820.1322172816830056No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21750.11586325046758145No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21620.11517073448777523No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20680.11016331124917629No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG20590.10968387710931045No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA20340.10835211560968308No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA19600.10441010157078605No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA19410.10339796283106925No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC19240.10249236501132264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1850.020.2161434
TTAACGG3750.018.77326435
TAACGGC4200.016.76184536
TACCGTC6300.016.412647
GCATTAG1351.0844578E-616.2988411
GTATTAG6500.016.2486921
GTATCAA50550.016.0183281
CGTCGTA6000.015.7666110
ATACCGT6300.015.7142296
CTAGATA4650.015.6128473
ACGATTA1704.494359E-815.52935640
CGCAATA6750.015.31846336
GTCCTAT7550.015.1546841
GTCGTAG6250.015.13594611
TATTAGA5600.014.9285172
CCTATTC8700.014.9194863
TACGCTA5700.014.6666149
CGAGCCG8700.014.66661415
AACGCAG55350.014.6268676
ATACGAA6800.014.55877140
Produced by FastQC (version 0.11.5)