##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1877213
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233823226240176	32.0	32.0	32.0	32.0	32.0
2	31.408168385793193	32.0	32.0	32.0	32.0	32.0
3	31.48805702922364	32.0	32.0	32.0	32.0	32.0
4	31.594662939155015	32.0	32.0	32.0	32.0	32.0
5	31.52569420731691	32.0	32.0	32.0	32.0	32.0
6	35.10842509613986	36.0	36.0	36.0	36.0	36.0
7	35.122182725135616	36.0	36.0	36.0	36.0	36.0
8	35.06296195477018	36.0	36.0	36.0	36.0	36.0
9	35.195909574459584	36.0	36.0	36.0	36.0	36.0
10	35.07067818089902	36.0	36.0	36.0	36.0	36.0
11	35.196472643221625	36.0	36.0	36.0	36.0	36.0
12	35.09915923233005	36.0	36.0	36.0	36.0	36.0
13	35.149254772900036	36.0	36.0	36.0	36.0	36.0
14	35.107670786426475	36.0	36.0	36.0	36.0	36.0
15	35.07308121134895	36.0	36.0	36.0	36.0	36.0
16	35.08444699669137	36.0	36.0	36.0	36.0	36.0
17	35.05385483693113	36.0	36.0	36.0	36.0	36.0
18	35.0671868349516	36.0	36.0	36.0	36.0	36.0
19	35.04884581557873	36.0	36.0	36.0	36.0	36.0
20	35.037737326558045	36.0	36.0	36.0	36.0	36.0
21	35.02900842898488	36.0	36.0	36.0	36.0	36.0
22	35.01426210025181	36.0	36.0	36.0	36.0	36.0
23	34.9814006188962	36.0	36.0	36.0	36.0	36.0
24	34.959477693793936	36.0	36.0	36.0	32.0	36.0
25	34.93658577902454	36.0	36.0	36.0	32.0	36.0
26	34.89464860940128	36.0	36.0	36.0	32.0	36.0
27	34.87923639991839	36.0	36.0	36.0	32.0	36.0
28	34.86452309887051	36.0	36.0	36.0	32.0	36.0
29	34.84054659753581	36.0	36.0	36.0	32.0	36.0
30	34.82873227492032	36.0	36.0	36.0	32.0	36.0
31	34.82803762812212	36.0	36.0	36.0	32.0	36.0
32	34.78781949624257	36.0	36.0	36.0	32.0	36.0
33	34.76245157049306	36.0	36.0	36.0	32.0	36.0
34	34.746522637548324	36.0	36.0	36.0	32.0	36.0
35	34.718810278854875	36.0	36.0	36.0	32.0	36.0
36	34.68570055715574	36.0	36.0	36.0	32.0	36.0
37	34.676575860064894	36.0	36.0	36.0	32.0	36.0
38	34.64056076747817	36.0	36.0	36.0	32.0	36.0
39	34.63774116203116	36.0	36.0	36.0	32.0	36.0
40	34.622455203538436	36.0	36.0	36.0	32.0	36.0
41	34.581324015974744	36.0	36.0	36.0	32.0	36.0
42	34.548342676084175	36.0	36.0	36.0	32.0	36.0
43	34.548021987915064	36.0	36.0	36.0	32.0	36.0
44	34.49939351581307	36.0	36.0	36.0	32.0	36.0
45	34.45772802553573	36.0	36.0	36.0	32.0	36.0
46	34.45619383628816	36.0	36.0	36.0	32.0	36.0
47	34.411215456104344	36.0	36.0	36.0	32.0	36.0
48	34.36715545865067	36.0	36.0	36.0	32.0	36.0
49	34.34594902123521	36.0	36.0	36.0	32.0	36.0
50	33.83708668115978	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	9.0
21	46.0
22	154.0
23	482.0
24	1340.0
25	3023.0
26	6525.0
27	11832.0
28	20015.0
29	30281.0
30	43224.0
31	60859.0
32	90015.0
33	146228.0
34	324490.0
35	1138686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4267520403042	18.584909580878524	11.62070321365893	26.367635165158347
2	16.536326122695556	20.870380058109863	35.90942362132911	26.683870197865467
3	18.135898849619515	24.653673946106046	28.90392897952008	28.306498224754357
4	12.388904396519946	15.862513131175382	36.16197850855418	35.5866039637505
5	14.643410204382773	36.557385869371245	33.03061506605803	15.768588860187949
6	33.845869462034166	35.74944824768365	16.79670956919371	13.607972721088471
7	29.879028112419846	30.728319055962217	21.10692819621428	18.285724635403653
8	28.45729280587765	31.854616391427076	19.817942875954937	19.870147926740334
9	27.91441354816955	13.758800945870286	18.9935825076856	39.33320299827457
10	16.363406816381517	26.145408112984512	31.092156297660416	26.399028772973555
11	37.537349251257055	21.171278911876275	22.239937609637263	19.051434227229407
12	24.800595350660792	23.972399509272524	28.230307375881157	22.996697764185527
13	29.3624111914844	19.276288838826495	25.53189222533618	25.829407744352935
14	23.89877973357312	19.743044609215897	24.957583396236867	31.400592260974115
15	25.643653650384906	26.649666287203424	22.357558785284354	25.349121277127317
16	26.130279302348747	25.541907071813373	23.459671331915985	24.868142293921895
17	24.103125218075945	25.413099099569415	25.171357752157054	25.312417930197583
18	25.126237672549678	24.32494341345388	26.285775775045238	24.2630431389512
19	25.677586933395414	24.80203365308039	25.5421734241133	23.9782059894109
20	25.870639080381398	23.857708208924613	25.000039952844986	25.271612757849006
21	27.299033194421728	23.779933337346375	24.395739854774074	24.525293613457823
22	25.930355266024684	24.148831272743156	24.745833317796116	25.174980143436045
23	24.591668606599253	23.800495734900622	25.57919639380294	26.028639264697183
24	24.86734323702212	24.95539930737748	25.027474239737312	25.149783215863092
25	25.021241595919058	24.132210889227807	25.39631890467411	25.450228610179025
26	24.68222838857391	24.93238646866392	25.76154117833192	24.623843964430247
27	25.620960434431257	24.62091408913107	24.79500195236236	24.963123524075318
28	24.503559265783903	24.324890142993898	25.84219265474935	25.329357936472846
29	24.61947578671147	24.65468756076162	25.636835031506816	25.089001621020095
30	24.521777763098807	24.849018198787245	25.925188031406133	24.704016006707814
31	25.027154616977406	24.701139401868623	24.686756377672644	25.584949603481334
32	24.622032768790756	24.703589843027935	24.783495533005578	25.890881855175728
33	24.480280074770416	24.230974322040176	25.414164508769115	25.87458109442029
34	25.161449446599825	24.346091786067962	25.737995635018507	24.7544631323137
35	26.064383743347186	24.210092301726014	25.22952909446078	24.49599486046602
36	24.74807067711549	24.83570058379097	24.965147801554753	25.45108093753879
37	25.85828033366485	24.647442778203647	24.602375969056254	24.891900919075248
38	24.691337637231364	24.365375692582568	25.603487723556146	25.339798946629926
39	25.077495201663318	24.416728416008198	24.80586912619932	25.69990725612917
40	25.361426753383874	24.551236327470562	25.5628956330475	24.52444128609806
41	24.327606936453137	24.947302197459745	25.795953895482292	24.92913697060483
42	25.609081121854576	25.17966794391473	25.1418991877853	24.069351746445395
43	24.73645771683874	24.00217769640419	25.456194901697355	25.805169685059713
44	24.37837368481893	24.562742746827347	25.30346849292009	25.755415075433636
45	24.49024165078763	24.824993221333965	25.372293927220834	25.312471200657573
46	24.330988897891608	24.63521681899371	25.47683771296887	25.556956570145818
47	24.502200338693942	24.328165560504385	25.85154252771798	25.318091573083684
48	25.30213673142046	25.16773536087807	24.818334413835828	24.711793493865642
49	24.177064616535258	25.112813516633437	25.28178741570616	25.42833445112515
50	23.950624649746537	25.437830143851624	25.206156789962986	25.405388416438846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	29.5
2	39.0
3	106.5
4	174.0
5	226.5
6	279.0
7	293.0
8	307.0
9	441.5
10	576.0
11	820.0
12	1064.0
13	1835.5
14	2607.0
15	3764.5
16	4922.0
17	6145.5
18	7369.0
19	8060.0
20	8751.0
21	9625.5
22	10500.0
23	10860.0
24	11220.0
25	12670.0
26	14120.0
27	16851.5
28	19583.0
29	22302.5
30	25022.0
31	26292.0
32	27562.0
33	32109.5
34	36657.0
35	42420.0
36	48183.0
37	59331.0
38	70479.0
39	76069.0
40	81659.0
41	90125.0
42	98591.0
43	99411.5
44	100232.0
45	109954.5
46	119677.0
47	126718.0
48	133759.0
49	137464.0
50	141169.0
51	134772.0
52	128375.0
53	126105.5
54	123836.0
55	126415.0
56	128994.0
57	126464.0
58	123934.0
59	113547.5
60	103161.0
61	91220.0
62	79279.0
63	69716.5
64	60154.0
65	52136.0
66	44118.0
67	38966.5
68	33815.0
69	31380.5
70	28946.0
71	23247.0
72	17548.0
73	16009.5
74	14471.0
75	10921.5
76	7372.0
77	6597.0
78	5822.0
79	5276.0
80	4730.0
81	3853.0
82	2976.0
83	2701.0
84	2426.0
85	2001.0
86	1576.0
87	1178.0
88	780.0
89	516.0
90	252.0
91	163.5
92	75.0
93	50.0
94	25.0
95	17.0
96	9.0
97	9.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015288622015722245
2	0.004208366338822499
3	4.261636798807594E-4
4	5.3270459985094925E-5
5	0.0
6	6.392455198211391E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0654091997018985E-4
47	1.0654091997018985E-4
48	0.0
49	0.0
50	5.3270459985094925E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1877213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.26697174238218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22036949185134	38.81634131183203
2	13.655777610653	13.728691745498992
3	4.126673630835079	6.223061603736618
4	1.6517051334789663	3.3210486108533916
5	0.8427153359604644	2.1180373989798387
6	0.5178347273635292	1.5617990164564026
7	0.3392535094538609	1.1937272601254851
8	0.2573962250424136	1.0350823016642279
9	0.17180909807460285	0.7772690770200208
>10	1.007128441198617	9.269585437866146
>50	0.09359877239576263	3.281512855757772
>100	0.09717801852079548	10.580724629344965
>500	0.013973337226891732	4.7506188846251245
>1k	0.004586667944704919	3.342499866239045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2482	0.1322172816830056	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2175	0.11586325046758145	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2162	0.11517073448777523	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2068	0.11016331124917629	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2059	0.10968387710931045	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2034	0.10835211560968308	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1960	0.10441010157078605	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1941	0.10339796283106925	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1924	0.10249236501132264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0654091997018985E-4
2	0.0	0.0	0.0	0.0	1.0654091997018985E-4
3	0.0	0.0	0.0	0.0	1.0654091997018985E-4
4	0.0	0.0	0.0	0.0	1.0654091997018985E-4
5	0.0	0.0	0.0	0.0	1.0654091997018985E-4
6	0.0	0.0	0.0	0.0	1.0654091997018985E-4
7	0.0	0.0	0.0	5.3270459985094925E-5	1.0654091997018985E-4
8	0.0	0.0	0.0	1.5981137995528478E-4	1.0654091997018985E-4
9	0.0	0.0	0.0	2.130818399403797E-4	1.0654091997018985E-4
10	0.0	0.0	0.0	2.130818399403797E-4	1.0654091997018985E-4
11	0.0	0.0	0.0	2.130818399403797E-4	1.0654091997018985E-4
12	0.0	0.0	0.0	3.7289321989566446E-4	1.5981137995528478E-4
13	0.0	0.0	0.0	3.7289321989566446E-4	1.5981137995528478E-4
14	0.0	0.0	0.0	4.261636798807594E-4	1.5981137995528478E-4
15	0.0	0.0	0.0	4.7943413986585435E-4	2.663522999254746E-4
16	0.0	0.0	0.0	5.859750598360442E-4	2.663522999254746E-4
17	0.0	0.0	0.0	5.859750598360442E-4	2.663522999254746E-4
18	0.0	0.0	0.0	6.925159798062341E-4	2.663522999254746E-4
19	0.0	0.0	0.0	6.925159798062341E-4	2.663522999254746E-4
20	0.0	0.0	0.0	7.457864397913289E-4	2.663522999254746E-4
21	0.0	0.0	0.0	7.990568997764239E-4	3.1962275991056957E-4
22	0.0	0.0	0.0	0.0012784910396422783	3.7289321989566446E-4
23	0.0	0.0	0.0	0.002077547939418702	3.7289321989566446E-4
24	0.0	0.0	0.0	0.0031962275991056954	3.7289321989566446E-4
25	0.0	0.0	0.0	0.0041018254188523095	3.7289321989566446E-4
26	0.0	0.0	0.0	0.005007423238598923	4.261636798807594E-4
27	0.0	0.0	0.0	0.006765348418107056	4.261636798807594E-4
28	0.0	0.0	0.0	0.01512881063576696	4.7943413986585435E-4
29	0.0	0.0	0.0	0.03180246461110167	4.7943413986585435E-4
30	0.0	0.0	0.0	0.06179373358271011	4.7943413986585435E-4
31	0.0	0.0	0.0	0.12934067684381048	4.7943413986585435E-4
32	0.0	0.0	0.0	0.20818095762175096	5.327045998509492E-4
33	0.0	0.0	0.0	0.29245482531817113	5.327045998509492E-4
34	0.0	0.0	0.0	0.3879154896114613	5.859750598360442E-4
35	0.0	0.0	0.0	0.49711993258090587	5.859750598360442E-4
36	0.0	0.0	0.0	0.647076277438948	6.392455198211391E-4
37	0.0	0.0	0.0	0.8610637151990743	6.392455198211391E-4
38	0.0	0.0	0.0	1.1463802988792429	6.392455198211391E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	185	0.0	20.216143	4
TTAACGG	375	0.0	18.773264	35
TAACGGC	420	0.0	16.761845	36
TACCGTC	630	0.0	16.41264	7
GCATTAG	135	1.0844578E-6	16.298841	1
GTATTAG	650	0.0	16.248692	1
GTATCAA	5055	0.0	16.018328	1
CGTCGTA	600	0.0	15.76661	10
ATACCGT	630	0.0	15.714229	6
CTAGATA	465	0.0	15.612847	3
ACGATTA	170	4.494359E-8	15.529356	40
CGCAATA	675	0.0	15.318463	36
GTCCTAT	755	0.0	15.154684	1
GTCGTAG	625	0.0	15.135946	11
TATTAGA	560	0.0	14.928517	2
CCTATTC	870	0.0	14.919486	3
TACGCTA	570	0.0	14.666614	9
CGAGCCG	870	0.0	14.666614	15
AACGCAG	5535	0.0	14.626867	6
ATACGAA	680	0.0	14.558771	40
>>END_MODULE