FastQCFastQC Report
Thu 2 Feb 2017
SRR4062451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855218
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG147431.7238879443603854No Hit
TCCATGTACTCTGCGTTGATACCAC122411.431330958890014No Hit
GTATCAACGCAGAGTACTTTTTTTT67820.7930141788409504No Hit
GAGTACATGGAAGCAGTGGTATCAA61030.7136192175562255No Hit
GTACTTTTTTTTTTTTTTTTTTTTT60970.7129176420515003No Hit
CATGTACTCTGCGTTGATACCACTG54000.6314179542525999No Hit
TATCAACGCAGAGTACTTTTTTTTT50150.5864001926994054No Hit
GAGTACTTTTTTTTTTTTTTTTTTT42950.502211132132392No Hit
GCTTCCATGTACTCTGCGTTGATAC37170.4346260251772063No Hit
GCGTTGATACCACTGCTTCCATGTA33590.39276535339527463No Hit
CCCATGTACTCTGCGTTGATACCAC32960.385398810595661No Hit
CATGGAAGCAGTGGTATCAACGCAG32490.37990313580864765No Hit
GTACATGGGAAGCAGTGGTATCAAC30470.3562834271495689No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30400.35546492239405625No Hit
GTATCAACGCAGAGTACATGGAAGC28600.33441765725230294No Hit
GTACATGGGGTGGTATCAACGCAAA27250.31863220839598794No Hit
GGTATCAACGCAGAGTACTTTTTTT24180.28273492840421977No Hit
ACGCAGAGTACATGGAAGCAGTGGT22410.262038451014829No Hit
ACTCTGCGTTGATACCACTGCTTCC21670.25338568645655263No Hit
CAGTGGTATCAACGCAGAGTACATG20400.23853567160653774No Hit
GTATCAACGCAGAGTACATGGGAAG19120.22356872750573537No Hit
TATCAACGCAGAGTACATGGAAGCA18670.21830691122029705No Hit
GTACATGGGTGGTATCAACGCAAAA16850.19702578757696868No Hit
GTACATGGGAGTGGTATCAACGCAA14240.16650725312142634No Hit
GCAGAGTACTTTTTTTTTTTTTTTT13750.16077771983283795No Hit
ATACCACTGCTTCCATGTACTCTGC13280.15528204504582457No Hit
GTATCAACGCAGAGTACATGGGGTG12690.148383219249361No Hit
TATCAACGCAGAGTACATGGGAAGC12490.1460446342336106No Hit
GGTATCAACGCAGAGTACATGGAAG12460.14569384648124806No Hit
GCGCAAGACGGACCAGAGCGAAAGC10710.1252312275934323No Hit
GCGTTGATACCACTGCTTCCCATGT10520.12300957182846946No Hit
AAGCAGTGGTATCAACGCAGAGTAC10470.12242492557453187No Hit
GGTATCAACGCAGAGTACATGGGAA10360.12113870381586916No Hit
GAATAGGACCGCGGTTCTATTTTGT10290.12032019906035654No Hit
GTACTCTGCGTTGATACCACTGCTT10250.11985248205720646No Hit
AAAAAGTACTCTGCGTTGATACCAC10240.11973555280641895No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10220.11950169430484392No Hit
GAGTACATGGGAAGCAGTGGTATCA10000.1169292507875185No Hit
CCACTGCTTCCATGTACTCTGCGTT9870.11540917052728077No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9730.1137721610162555No Hit
GAATAACGCCGCCGCATCGCCAGTC9650.11283672700995535No Hit
GTGGTATCAACGCAGAGTACATGGA9430.11026428349262996No Hit
GATTAAGAGGGACGGCCGGGGGCAT9180.10734105222294198No Hit
GAACTACGACGGTATCTGATCGTCT8730.10207923593750366No Hit
TATCAACGCAGAGTACATGGGGTGG8700.1017284481851411No Hit
GTATCTGATCGTCTTCGAACCTCCG8580.10032529717569087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG1250.015.20220113
ATCGCCA1400.014.25456716
TTAGCGG400.005283758514.247065
GCATCGC1350.014.07693714
CGCCACT1101.8371793E-1013.8234217
TAGGACG1051.371518E-913.5678344
CGGTCCA1650.013.24279110
TCGCGTA655.4454693E-513.154219
TGTTAGA655.4754517E-513.1465152
TCGCCAG1450.013.10841617
TCGAACG802.0062962E-613.0567473
GTAGGAC957.414019E-812.9942763
CGACTTT751.4745414E-512.66850113
TTAGAGT1207.403287E-1012.6633114
GTCTTGA604.1140357E-412.6573871
ACTCTAA1600.012.46909610
AATTAGA1301.9826984E-1012.4161532
GTCCTGC853.969846E-612.285111
CCGCATC1551.8189894E-1212.25983912
GCGTAAC701.08891436E-412.2153411
Produced by FastQC (version 0.11.5)