FastQCFastQC Report
Thu 2 Feb 2017
SRR4062450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611243
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG51050.8351833886032233No Hit
TCCATGTACTCTGCGTTGATACCAC40810.6676559077159165No Hit
GTATCAACGCAGAGTACTTTTTTTT40180.6573490412160139No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37680.6164487773275112No Hit
GTATTAGAGGCACTGCCTGCCCAGT34430.5632784342724579No Hit
CCATAGGGTCTTCTCGTCTTATTAT33080.5411922917726665No Hit
TATCAACGCAGAGTACTTTTTTTTT29450.48180510860656073No Hit
CTCTAATACTTGTAATGCTAGAGGT27830.45530173760681103No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25520.41750989377383463No Hit
CCATTGGGATGTCCTGATCCAACAT23040.37693683199644No Hit
GAGTACATGGAAGCAGTGGTATCAA20770.3397993923856797No Hit
GTCAGGATACCGCGGCCGTTAAACT20450.33456415860795136No Hit
ATCTATAACTTTATAGATGCAACAC20310.3322737438301952No Hit
CTTCTACACCATTGGGATGTCCTGA19930.3260569037191428No Hit
GATATATTTTGATCAACGGACCAAG19410.31754964883033426No Hit
GTTATATAATTTAAGCTCCATAGGG18570.3038071601637974No Hit
CCTCTAGCATTACAAGTATTAGAGG18500.3026619527749193No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18330.2998807348305011No Hit
CTGTTAGTATGAGTAACAAGAATTC17940.29350029366389474No Hit
ATCCTGACCGTGCAAAGGTAGCATA17810.29137347994169255No Hit
GTTATAGATTAACCCAATTTTAAGT17270.282539022941776No Hit
GTATCCTGACCGTGCAAAGGTAGCA16740.27386816699741345No Hit
CATGTACTCTGCGTTGATACCACTG16020.2620888909975247No Hit
GGTCAGGATACCGCGGCCGTTAAAC15870.2596348751642146No Hit
CCTATAACTTCTCTGTTAACCCAAC15650.2560356519420264No Hit
ACCTATAACTTCTCTGTTAACCCAA15650.2560356519420264No Hit
GTTCATGCTAGTCCCTAATTAAGGA15540.2542360403309322No Hit
GTACATGGGGTGGTATCAACGCAAA15160.2480192002198798No Hit
CTATAGAACTAGTACCGCAAGGGAA15090.24687399283100175No Hit
GTACATGGGAGAAATCGTAAATAGA15060.24638318966433972No Hit
TGCTAGAGGTGATGTTTTTGGTAAA15010.24556518438656966No Hit
ATCGTAAATAGATAGAAACCGACCT14940.24441997699769158No Hit
ATAAATAATCCACCTATAACTTCTC14880.24343837066436755No Hit
GCCTAAAGGAAAGATCCAAAAAGAT14670.2400027484977333No Hit
CACTATAAATAATCCACCTATAACT14480.2368943284422071No Hit
GGTATCAACGCAGAGTACTTTTTTT14440.23623992421999107No Hit
GACTATAGGCAATAATCACACTATA14280.2336223073311269No Hit
GATTAAACCTTGTACCTTTTGCATA14190.2321498978311408No Hit
GATTAAAGATAAGAGACAGTTGGAC14110.23084108938670875No Hit
GTATCAACGCAGAGTACATGGGAGA13080.21399018066464562No Hit
TCCCAATGGTGTAGAAGCTATTAAT12980.21235417010910554No Hit
AAATATATCTGGGTCAATAAGATAT12920.21137256377578148No Hit
GATAGAAACCGACCTGGATTGCTCC12760.20875494688691731No Hit
CTGTTAACCCAACACCGGAATGCCT12260.2005748941092168No Hit
CATGGAAGCAGTGGTATCAACGCAG12030.19681206983147456No Hit
TACTAACAGTGTTGCATCTATAAAG11890.1945216550537184No Hit
GCTTCCATGTACTCTGCGTTGATAC11810.19321284660928634No Hit
GTGTAGAAGCTATTAATGGTTCGTT11480.18781401177600396No Hit
CCTATAGTCTGATTAACTAACAATG11450.18732320860934196No Hit
GTTAGTATGAGTAACAAGAATTCCA11420.18683240544267993No Hit
CTATTAAAGGTTTTTTCCGTTCCAG11420.18683240544267993No Hit
GTCCTGATCCAACATCGAGGTCGTA11400.1865052033315719No Hit
ACCTTTGCACGGTCAGGATACCGCG11260.18421478855381576No Hit
AGCATGAACGGCTAAACGAGGGTCC11230.1837239853871537No Hit
ATTATAACCTAGACTTACAAGTCAA10720.17538033155389918No Hit
CCATTAATAGCTTCTACACCATTGG10720.17538033155389918No Hit
ACCCTATGGAGCTTAAATTATATAA10690.17488952838723715No Hit
GACCTGGATTGCTCCGGTCTGAACT10640.1740715231094671No Hit
CTTAAATAGGATTGCGCTGTTATCC10620.17374432099835907No Hit
GCGTTGATACCACTGCTTCCATGTA10470.17129030516504892No Hit
GGATTGCTCCGGTCTGAACTCAGAT10400.17014509777617087No Hit
CTTTATTGGTGGCTGCTTTTAGGCC10390.16998149672061685No Hit
GTTATAGGTGGATTATTTATAGTGT10200.16687307666509063No Hit
CTATTATATAAATCAAAACATTTAT10200.16687307666509063No Hit
GTCCTAAAGTGTGTATTTCTCATTT10130.16572786927621258No Hit
CCAATAAAGAAAGCGTTCAAGCTCA10130.16572786927621258No Hit
ATCCTAGCCCTAGCCCTACACAAAT10130.16572786927621258No Hit
GGATACCGCGGCCGTTAAACTTTAG9990.16343745449845642No Hit
ATTTAAGAGTTCATATCGACAATTA9950.16278305027624038No Hit
CCCATGTACTCTGCGTTGATACCAC9800.16032903444293023No Hit
GTTATACGCGTATGCCTGGAGAATT9720.15902022599849813No Hit
AGGGATAACAGCGCAATCCTATTTA9540.15607540699852596No Hit
CAGTTGGACCCTCGTTTAGCCGTTC9480.1550938006652019No Hit
GTACATGGGTGGTATCAACGCAAAA9400.1537849922207698No Hit
GTTTAGATTATAGCCAAAAGAGGGA9180.15018576899858158No Hit
ATTTCAGCCTCTTCACTGAAAGGTC9130.1493677637208115No Hit
CCTTTAGGCATTCCGGTGTTGGGTT9120.14920416266525752No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9010.1474045510541634No Hit
GTTTAAAATTGAACTTAAATTCATT8870.14511413627640724No Hit
GGGCAGGCAGTGCCTCTAATACTTG8820.1442961309986372No Hit
TTATATAACTTATCTATTTAATTTA8810.1441325299430832No Hit
CTTTAGGACGTGAAATATGGCGAGG8760.14331452466531314No Hit
GTTATCCCTAGGGTAACTTGGTCCG8740.1429873225542051No Hit
CTCTTAAATAGGATTGCGCTGTTAT8720.14266012044309712No Hit
CTATAGTATAAGTTTGAAATTTCGG8690.14216931727643506No Hit
GTATCAACGCAGAGTACATGGAAGC8580.14036970566534096No Hit
GATAAAAGGAACTCGGCAAACAAGA8580.14036970566534096No Hit
GCTATCACCAAGCTCGTTAGGCTTT8460.13840649299869284No Hit
AATCTAAACTTACTTTTTGATTTTG8400.13742488666536876No Hit
GGTTTAATCTTTGCTTGTTCTTACT8090.13235325394319444No Hit
ACTAAAAGTATTGGAGAAAGAAATT8080.13218965288764045No Hit
GTCCTACAGTGGACATTTCTAAATT8020.13120804655431637No Hit
CCTTTAATAGTGAATAATTAACAAA8010.13104444549876235No Hit
CTGTAGGACGTGGAATATGGCAAGA7980.13055364233210032No Hit
GTTCTATAGCTCCTAGATGTACGAA7950.13006283916543832No Hit
ACGTAGGACTTTAATCGTTGAACAA7930.12973563705433028No Hit
CCCTAATTGTCGATATGAACTCTTA7850.12842682860989818No Hit
CAATTAGGGTTTACGACCTCGATGT7800.12760882333212814No Hit
GTATAAGTTTGAAATTTCGGTTGGG7790.12744522227657412No Hit
TATCAACGCAGAGTACATGGGAGAA7710.12613641383214202No Hit
ATGCAACACTGTTAGTATGAGTAAC7710.12613641383214202No Hit
GGTTATAATCATTCGGAGGATTTTT7710.12613641383214202No Hit
GGTCTGAACTCAGATCACGTAGGAC7660.12531840855437199No Hit
GCGGTATCCTGACCGTGCAAAGGTA7530.12319159483216986No Hit
CATTTATCCTACTAAAAGTATTGGA7450.12188278638773777No Hit
GTATCAACGCAGAGTACATGGGAAG7440.12171918533218377No Hit
TGCTAGTCCCTAATTAAGGAACAAG7360.12041037688775168No Hit
ATTTTAAACTTGCTAAAAAAACAAC7340.12008317477664365No Hit
CTATAAATAATCCACCTATAACTTC7320.11975597266553563No Hit
GCTTTTAACCATTGTAGGCCTAAAA7280.1191015684433196No Hit
CTGCCTGCCCAGTGACTAAAGTTTA7200.1177927599988875No Hit
TCTTTAATCAGTGAAATTGACCTTT7150.11697475472111747No Hit
CGTCTATGTGGCAAAATAGTGAGAA7140.11681115366556345No Hit
GGGATGTCCTGATCCAACATCGAGG7130.11664755261000945No Hit
GCGTATAACAACTCGGATAACCATT7080.11582954733223938No Hit
CTATTTAAGAGTTCATATCGACAAT7010.11468433994336132No Hit
AAGTTACCCTAGGGATAACAGCGCA6910.11304832938782122No Hit
CGTTTAGCCGTTCATGCTAGTCCCT6860.11223032411005116No Hit
GTGCAAAGGTAGCATAATCACTTGT6830.11173952094338913No Hit
ACTCTGCGTTGATACCACTGCTTCC6800.11124871777672708No Hit
ACCTTGTACCTTTTGCATAATGAAC6760.11059431355451106No Hit
TAATCACACTATAAATAATCCACCT6720.10993990933229501No Hit
AACCTAGACTTACAAGTCAAAGTAA6700.10961270722118699No Hit
ACGCAGAGTACATGGAAGCAGTGGT6680.10928550511007896No Hit
ACTTTATAGATGCAACACTGTTAGT6630.10846749983230893No Hit
TCTAGGAGCTATAGAACTAGTACCG6630.10846749983230893No Hit
GTACATGGGAGTGGTATCAACGCAA6610.1081402977212009No Hit
ATATAAATCAAAACATTTATCCTAC6580.10764949455453886No Hit
GTATCAACGCAGAGTACATGGGGTG6490.10617708505455277No Hit
CTTCTCTGTTAACCCAACACCGGAA6470.10584988294344475No Hit
GATTATAACCTAGACTTACAAGTCA6450.10552268083233672No Hit
CAAGAACCCCGCCTGTTTACCAAAA6390.10454107449901268No Hit
GTACATGGGAAGCAGTGGTATCAAC6350.10388667027679663No Hit
TATCAACGCAGAGTACATGGAAGCA6180.10110545233237844No Hit
ACCCTAGGGATAACAGCGCAATCCT6170.10094185127682442No Hit
ATCTTATTGACCCAGATATATTTTG6160.10077825022127042No Hit
CATACTAACAGTGTTGCATCTATAA6150.10061464916571643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGC250.006025118319.00155611
AGTAGGG307.7281526E-418.9953295
TGAACTT1150.017.34782610
TTGCTAA1550.017.16129110
AACTTGC1450.017.035887
ACCTTGA453.530723E-516.8902748
ACTTGCT1500.016.4680168
CCTTTTT1001.8189894E-1216.1539716
TGCTAAA1650.016.12253411
AAACTTG1700.015.6483416
GAACTTA1350.015.4827511
CGGACCA2150.015.46891917
AAATTCA1300.015.34992717
GGACCTG2050.015.2939386
CCAACGT508.700123E-515.219
ACGGACC2200.015.11735316
CTTTTTC953.0195224E-1015.00368917
CACCTTT953.0195224E-1015.0024614
CCACCTT953.0195224E-1015.0024613
CATTCCG1400.014.9285729
Produced by FastQC (version 0.11.5)