##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062450_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	611243
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.857295707271902	32.0	32.0	32.0	32.0	32.0
2	30.659549475413215	32.0	32.0	32.0	32.0	32.0
3	30.722550605896508	32.0	32.0	32.0	32.0	32.0
4	30.693063151643454	32.0	32.0	32.0	32.0	32.0
5	30.642500609413933	32.0	32.0	32.0	32.0	32.0
6	34.164270183871224	36.0	36.0	36.0	32.0	36.0
7	33.918341805141324	36.0	36.0	36.0	32.0	36.0
8	33.861613139127975	36.0	36.0	36.0	32.0	36.0
9	33.9827531767235	36.0	36.0	36.0	32.0	36.0
10	33.78356234754427	36.0	36.0	36.0	32.0	36.0
11	34.18033417151607	36.0	36.0	36.0	32.0	36.0
12	33.94397809054664	36.0	36.0	36.0	32.0	36.0
13	34.08702267347029	36.0	36.0	36.0	32.0	36.0
14	33.975783771756895	36.0	36.0	36.0	32.0	36.0
15	33.97870568660909	36.0	36.0	36.0	32.0	36.0
16	33.9561336489743	36.0	36.0	36.0	32.0	36.0
17	33.90748196707365	36.0	36.0	36.0	32.0	36.0
18	33.97677192213244	36.0	36.0	36.0	32.0	36.0
19	33.91148528490306	36.0	36.0	36.0	32.0	36.0
20	33.837230037808204	36.0	36.0	36.0	32.0	36.0
21	33.77941342477542	36.0	36.0	36.0	32.0	36.0
22	33.72887051467256	36.0	36.0	36.0	27.0	36.0
23	33.76584762524888	36.0	36.0	36.0	32.0	36.0
24	33.735298400145275	36.0	36.0	36.0	27.0	36.0
25	33.31646824585312	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	20.0
5	59.0
6	107.0
7	19.0
8	95.0
9	107.0
10	77.0
11	30.0
12	51.0
13	28.0
14	326.0
15	303.0
16	431.0
17	572.0
18	805.0
19	1054.0
20	1512.0
21	2223.0
22	2990.0
23	3952.0
24	4885.0
25	6326.0
26	8236.0
27	9931.0
28	13088.0
29	16887.0
30	21939.0
31	29616.0
32	41327.0
33	59014.0
34	130881.0
35	254348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.05499971367098	23.12245882997783	12.607148408419711	23.215393047931478
2	13.423207493149004	23.182134238619167	42.016605996155256	21.378052272076566
3	15.742802497958996	29.49515640057001	33.09632063139398	21.66572047007701
4	8.672488468479965	19.680569940980217	43.9876560784487	27.65928551209112
5	8.860910721533406	42.43051100433365	38.272463635292425	10.436114638840518
6	24.566106910209825	44.212085501980425	20.522275688238565	10.69953189957118
7	24.151451205477077	37.236120274312135	23.601058874953548	15.011369645257243
8	24.249852221911112	36.21979555044677	22.243181330674147	17.28717089696797
9	25.333254727795573	16.960291102508172	23.777028841681897	33.929425328014354
10	14.004055585766794	27.84153077069233	37.03064293155298	21.12377071198789
11	28.76967227077955	25.896800020966282	28.644202167389565	16.689325540864598
12	21.31746689623199	30.68219235979942	31.891505809848486	16.108834934120104
13	23.80829142847783	24.16799069630309	30.639301567541892	21.38441630767719
14	19.402843275844276	26.831429680602398	29.58264877729966	24.183078266253666
15	22.002951393722803	31.28739401106199	28.36417119393867	18.345483401276535
16	21.18589103154143	30.62260671315935	28.728122614902336	19.46337964039688
17	17.251766602128768	30.292025802055385	31.633398526439244	20.822809069376603
18	17.847348952719194	28.839947709677315	34.54126381385169	18.7714395237518
19	20.07011278657373	31.348278714708123	30.956269606598465	17.625338892119682
20	18.70018443838022	30.759440591513215	32.6446105008665	17.89576446924007
21	21.053079919664242	28.382898836573084	29.43001582470292	21.134005419059758
22	18.79082905824659	31.916751731471578	29.498672005950986	19.79374720433085
23	19.563222910318164	31.17828238146728	30.69088109046823	18.56761361774632
24	19.152411314288337	32.99383696966321	29.69230718823618	18.16144452781227
25	19.782650005569717	30.59428874720695	31.553426686149756	18.06963456107358
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	412.0
1	412.0
2	857.0
3	1302.0
4	1302.0
5	1302.0
6	4407.0
7	7512.0
8	7512.0
9	7512.0
10	7034.5
11	6557.0
12	6557.0
13	6557.0
14	6523.5
15	6490.0
16	6490.0
17	6490.0
18	10399.0
19	14308.0
20	14308.0
21	14308.0
22	21591.0
23	28874.0
24	28874.0
25	28874.0
26	43057.5
27	57241.0
28	57241.0
29	57241.0
30	64624.0
31	72007.0
32	72007.0
33	72007.0
34	75512.5
35	79018.0
36	79018.0
37	79018.0
38	73963.0
39	68908.0
40	68908.0
41	68908.0
42	63189.0
43	57470.0
44	57470.0
45	57470.0
46	74384.0
47	91298.0
48	91298.0
49	91298.0
50	74970.5
51	58643.0
52	58643.0
53	58643.0
54	45131.5
55	31620.0
56	31620.0
57	31620.0
58	22810.0
59	14000.0
60	14000.0
61	14000.0
62	10273.5
63	6547.0
64	6547.0
65	6547.0
66	5344.5
67	4142.0
68	4142.0
69	4142.0
70	3304.5
71	2467.0
72	2467.0
73	2467.0
74	1836.5
75	1206.0
76	1206.0
77	1206.0
78	889.5
79	573.0
80	573.0
81	573.0
82	389.0
83	205.0
84	205.0
85	205.0
86	152.0
87	99.0
88	99.0
89	99.0
90	64.5
91	30.0
92	30.0
93	30.0
94	27.0
95	24.0
96	24.0
97	24.0
98	157.0
99	290.0
100	290.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009488861222132605
2	0.0029448189999721876
3	0.003926425333296251
4	0.01505129711096896
5	0.0345198227218962
6	0.04302707761070475
7	0.06642202855492824
8	0.08670855944362553
9	0.09799703227685226
10	0.11893796738776559
11	0.1209011800544137
12	0.13317125922096448
13	0.11991957372108963
14	0.12270079166550782
15	0.11190312199894313
16	0.1114123188322811
17	0.12253719060995381
18	0.1316988497209784
19	0.13055364233210032
20	0.12122838216552173
21	0.1316988497209784
22	0.15116737533190566
23	0.141187710943111
24	0.13562527505427466
25	0.13071724338765434
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	611243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.431553935992024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.38921631658086	14.994557314924077
2	12.997060134491512	5.311002702943902
3	4.526513203937907	2.774510960047122
4	2.161828144925845	1.7667803337359196
5	1.2453452171079151	1.2722168986135034
6	0.8605038827182354	1.054885889513291
7	0.5951712746466794	0.8512191799367728
8	0.4053022726097972	0.6624764194565771
9	0.30917863068162194	0.5685299881764946
>10	2.4509895553370207	10.06558165877078
>50	0.37966301659628787	5.522273515914136
>100	0.5382564285922056	24.103650339762012
>500	0.08730647428058096	12.412841509855715
>1k	0.0528644706653059	17.804029763612217
>5k	8.009768282622106E-4	0.835443524737484
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5105	0.8351833886032233	No Hit
TCCATGTACTCTGCGTTGATACCAC	4081	0.6676559077159165	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4018	0.6573490412160139	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3768	0.6164487773275112	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	3443	0.5632784342724579	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	3308	0.5411922917726665	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2945	0.48180510860656073	No Hit
CTCTAATACTTGTAATGCTAGAGGT	2783	0.45530173760681103	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2552	0.41750989377383463	No Hit
CCATTGGGATGTCCTGATCCAACAT	2304	0.37693683199644	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2077	0.3397993923856797	No Hit
GTCAGGATACCGCGGCCGTTAAACT	2045	0.33456415860795136	No Hit
ATCTATAACTTTATAGATGCAACAC	2031	0.3322737438301952	No Hit
CTTCTACACCATTGGGATGTCCTGA	1993	0.3260569037191428	No Hit
GATATATTTTGATCAACGGACCAAG	1941	0.31754964883033426	No Hit
GTTATATAATTTAAGCTCCATAGGG	1857	0.3038071601637974	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1850	0.3026619527749193	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1833	0.2998807348305011	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1794	0.29350029366389474	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	1781	0.29137347994169255	No Hit
GTTATAGATTAACCCAATTTTAAGT	1727	0.282539022941776	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	1674	0.27386816699741345	No Hit
CATGTACTCTGCGTTGATACCACTG	1602	0.2620888909975247	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	1587	0.2596348751642146	No Hit
CCTATAACTTCTCTGTTAACCCAAC	1565	0.2560356519420264	No Hit
ACCTATAACTTCTCTGTTAACCCAA	1565	0.2560356519420264	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	1554	0.2542360403309322	No Hit
GTACATGGGGTGGTATCAACGCAAA	1516	0.2480192002198798	No Hit
CTATAGAACTAGTACCGCAAGGGAA	1509	0.24687399283100175	No Hit
GTACATGGGAGAAATCGTAAATAGA	1506	0.24638318966433972	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	1501	0.24556518438656966	No Hit
ATCGTAAATAGATAGAAACCGACCT	1494	0.24441997699769158	No Hit
ATAAATAATCCACCTATAACTTCTC	1488	0.24343837066436755	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	1467	0.2400027484977333	No Hit
CACTATAAATAATCCACCTATAACT	1448	0.2368943284422071	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1444	0.23623992421999107	No Hit
GACTATAGGCAATAATCACACTATA	1428	0.2336223073311269	No Hit
GATTAAACCTTGTACCTTTTGCATA	1419	0.2321498978311408	No Hit
GATTAAAGATAAGAGACAGTTGGAC	1411	0.23084108938670875	No Hit
GTATCAACGCAGAGTACATGGGAGA	1308	0.21399018066464562	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	1298	0.21235417010910554	No Hit
AAATATATCTGGGTCAATAAGATAT	1292	0.21137256377578148	No Hit
GATAGAAACCGACCTGGATTGCTCC	1276	0.20875494688691731	No Hit
CTGTTAACCCAACACCGGAATGCCT	1226	0.2005748941092168	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1203	0.19681206983147456	No Hit
TACTAACAGTGTTGCATCTATAAAG	1189	0.1945216550537184	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1181	0.19321284660928634	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	1148	0.18781401177600396	No Hit
CCTATAGTCTGATTAACTAACAATG	1145	0.18732320860934196	No Hit
GTTAGTATGAGTAACAAGAATTCCA	1142	0.18683240544267993	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	1142	0.18683240544267993	No Hit
GTCCTGATCCAACATCGAGGTCGTA	1140	0.1865052033315719	No Hit
ACCTTTGCACGGTCAGGATACCGCG	1126	0.18421478855381576	No Hit
AGCATGAACGGCTAAACGAGGGTCC	1123	0.1837239853871537	No Hit
ATTATAACCTAGACTTACAAGTCAA	1072	0.17538033155389918	No Hit
CCATTAATAGCTTCTACACCATTGG	1072	0.17538033155389918	No Hit
ACCCTATGGAGCTTAAATTATATAA	1069	0.17488952838723715	No Hit
GACCTGGATTGCTCCGGTCTGAACT	1064	0.1740715231094671	No Hit
CTTAAATAGGATTGCGCTGTTATCC	1062	0.17374432099835907	No Hit
GCGTTGATACCACTGCTTCCATGTA	1047	0.17129030516504892	No Hit
GGATTGCTCCGGTCTGAACTCAGAT	1040	0.17014509777617087	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	1039	0.16998149672061685	No Hit
GTTATAGGTGGATTATTTATAGTGT	1020	0.16687307666509063	No Hit
CTATTATATAAATCAAAACATTTAT	1020	0.16687307666509063	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1013	0.16572786927621258	No Hit
CCAATAAAGAAAGCGTTCAAGCTCA	1013	0.16572786927621258	No Hit
ATCCTAGCCCTAGCCCTACACAAAT	1013	0.16572786927621258	No Hit
GGATACCGCGGCCGTTAAACTTTAG	999	0.16343745449845642	No Hit
ATTTAAGAGTTCATATCGACAATTA	995	0.16278305027624038	No Hit
CCCATGTACTCTGCGTTGATACCAC	980	0.16032903444293023	No Hit
GTTATACGCGTATGCCTGGAGAATT	972	0.15902022599849813	No Hit
AGGGATAACAGCGCAATCCTATTTA	954	0.15607540699852596	No Hit
CAGTTGGACCCTCGTTTAGCCGTTC	948	0.1550938006652019	No Hit
GTACATGGGTGGTATCAACGCAAAA	940	0.1537849922207698	No Hit
GTTTAGATTATAGCCAAAAGAGGGA	918	0.15018576899858158	No Hit
ATTTCAGCCTCTTCACTGAAAGGTC	913	0.1493677637208115	No Hit
CCTTTAGGCATTCCGGTGTTGGGTT	912	0.14920416266525752	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	901	0.1474045510541634	No Hit
GTTTAAAATTGAACTTAAATTCATT	887	0.14511413627640724	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	882	0.1442961309986372	No Hit
TTATATAACTTATCTATTTAATTTA	881	0.1441325299430832	No Hit
CTTTAGGACGTGAAATATGGCGAGG	876	0.14331452466531314	No Hit
GTTATCCCTAGGGTAACTTGGTCCG	874	0.1429873225542051	No Hit
CTCTTAAATAGGATTGCGCTGTTAT	872	0.14266012044309712	No Hit
CTATAGTATAAGTTTGAAATTTCGG	869	0.14216931727643506	No Hit
GTATCAACGCAGAGTACATGGAAGC	858	0.14036970566534096	No Hit
GATAAAAGGAACTCGGCAAACAAGA	858	0.14036970566534096	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	846	0.13840649299869284	No Hit
AATCTAAACTTACTTTTTGATTTTG	840	0.13742488666536876	No Hit
GGTTTAATCTTTGCTTGTTCTTACT	809	0.13235325394319444	No Hit
ACTAAAAGTATTGGAGAAAGAAATT	808	0.13218965288764045	No Hit
GTCCTACAGTGGACATTTCTAAATT	802	0.13120804655431637	No Hit
CCTTTAATAGTGAATAATTAACAAA	801	0.13104444549876235	No Hit
CTGTAGGACGTGGAATATGGCAAGA	798	0.13055364233210032	No Hit
GTTCTATAGCTCCTAGATGTACGAA	795	0.13006283916543832	No Hit
ACGTAGGACTTTAATCGTTGAACAA	793	0.12973563705433028	No Hit
CCCTAATTGTCGATATGAACTCTTA	785	0.12842682860989818	No Hit
CAATTAGGGTTTACGACCTCGATGT	780	0.12760882333212814	No Hit
GTATAAGTTTGAAATTTCGGTTGGG	779	0.12744522227657412	No Hit
TATCAACGCAGAGTACATGGGAGAA	771	0.12613641383214202	No Hit
ATGCAACACTGTTAGTATGAGTAAC	771	0.12613641383214202	No Hit
GGTTATAATCATTCGGAGGATTTTT	771	0.12613641383214202	No Hit
GGTCTGAACTCAGATCACGTAGGAC	766	0.12531840855437199	No Hit
GCGGTATCCTGACCGTGCAAAGGTA	753	0.12319159483216986	No Hit
CATTTATCCTACTAAAAGTATTGGA	745	0.12188278638773777	No Hit
GTATCAACGCAGAGTACATGGGAAG	744	0.12171918533218377	No Hit
TGCTAGTCCCTAATTAAGGAACAAG	736	0.12041037688775168	No Hit
ATTTTAAACTTGCTAAAAAAACAAC	734	0.12008317477664365	No Hit
CTATAAATAATCCACCTATAACTTC	732	0.11975597266553563	No Hit
GCTTTTAACCATTGTAGGCCTAAAA	728	0.1191015684433196	No Hit
CTGCCTGCCCAGTGACTAAAGTTTA	720	0.1177927599988875	No Hit
TCTTTAATCAGTGAAATTGACCTTT	715	0.11697475472111747	No Hit
CGTCTATGTGGCAAAATAGTGAGAA	714	0.11681115366556345	No Hit
GGGATGTCCTGATCCAACATCGAGG	713	0.11664755261000945	No Hit
GCGTATAACAACTCGGATAACCATT	708	0.11582954733223938	No Hit
CTATTTAAGAGTTCATATCGACAAT	701	0.11468433994336132	No Hit
AAGTTACCCTAGGGATAACAGCGCA	691	0.11304832938782122	No Hit
CGTTTAGCCGTTCATGCTAGTCCCT	686	0.11223032411005116	No Hit
GTGCAAAGGTAGCATAATCACTTGT	683	0.11173952094338913	No Hit
ACTCTGCGTTGATACCACTGCTTCC	680	0.11124871777672708	No Hit
ACCTTGTACCTTTTGCATAATGAAC	676	0.11059431355451106	No Hit
TAATCACACTATAAATAATCCACCT	672	0.10993990933229501	No Hit
AACCTAGACTTACAAGTCAAAGTAA	670	0.10961270722118699	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	668	0.10928550511007896	No Hit
ACTTTATAGATGCAACACTGTTAGT	663	0.10846749983230893	No Hit
TCTAGGAGCTATAGAACTAGTACCG	663	0.10846749983230893	No Hit
GTACATGGGAGTGGTATCAACGCAA	661	0.1081402977212009	No Hit
ATATAAATCAAAACATTTATCCTAC	658	0.10764949455453886	No Hit
GTATCAACGCAGAGTACATGGGGTG	649	0.10617708505455277	No Hit
CTTCTCTGTTAACCCAACACCGGAA	647	0.10584988294344475	No Hit
GATTATAACCTAGACTTACAAGTCA	645	0.10552268083233672	No Hit
CAAGAACCCCGCCTGTTTACCAAAA	639	0.10454107449901268	No Hit
GTACATGGGAAGCAGTGGTATCAAC	635	0.10388667027679663	No Hit
TATCAACGCAGAGTACATGGAAGCA	618	0.10110545233237844	No Hit
ACCCTAGGGATAACAGCGCAATCCT	617	0.10094185127682442	No Hit
ATCTTATTGACCCAGATATATTTTG	616	0.10077825022127042	No Hit
CATACTAACAGTGTTGCATCTATAA	615	0.10061464916571643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.6360105555401043E-4	0.0
3	0.0	0.0	0.0	1.6360105555401043E-4	0.0
4	0.0	0.0	0.0	1.6360105555401043E-4	0.0
5	0.0	0.0	0.0	1.6360105555401043E-4	0.0
6	0.0	0.0	0.0	1.6360105555401043E-4	0.0
7	0.0	0.0	0.0	6.544042222160417E-4	0.0
8	0.0	0.0	0.0	6.544042222160417E-4	0.0
9	0.0	0.0	0.0	0.001145207388878073	0.0
10	0.0	0.0	0.0	0.001145207388878073	0.0
11	0.0	0.0	0.0	0.0014724094999860938	0.0
12	0.0	0.0	0.0	0.0014724094999860938	0.0
13	0.0	0.0	0.0	0.0016360105555401044	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGC	25	0.0060251183	19.001556	11
AGTAGGG	30	7.7281526E-4	18.995329	5
TGAACTT	115	0.0	17.347826	10
TTGCTAA	155	0.0	17.161291	10
AACTTGC	145	0.0	17.03588	7
ACCTTGA	45	3.530723E-5	16.890274	8
ACTTGCT	150	0.0	16.468016	8
CCTTTTT	100	1.8189894E-12	16.15397	16
TGCTAAA	165	0.0	16.122534	11
AAACTTG	170	0.0	15.648341	6
GAACTTA	135	0.0	15.48275	11
CGGACCA	215	0.0	15.468919	17
AAATTCA	130	0.0	15.349927	17
GGACCTG	205	0.0	15.293938	6
CCAACGT	50	8.700123E-5	15.2	19
ACGGACC	220	0.0	15.117353	16
CTTTTTC	95	3.0195224E-10	15.003689	17
CACCTTT	95	3.0195224E-10	15.00246	14
CCACCTT	95	3.0195224E-10	15.00246	13
CATTCCG	140	0.0	14.928572	9
>>END_MODULE