FastQCFastQC Report
Thu 2 Feb 2017
SRR4062450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611243
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT34510.56458724271689No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG31760.5195969524395372No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT26130.4274895581626293No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA22960.3756280235520079No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22350.3656483591632133No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC20420.3340733554412893No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA19680.32196687733029256No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC19490.31885845727476636No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC19200.31411402666370003No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC18660.30527956966378345No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC18250.29857192638606905No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA17690.2894102672750445No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT17250.282211820830668No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT16890.27632218283072363No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA16480.2696145395530092No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC16190.26487010894194285No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA15960.26110728466420063No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC15510.25374523716427017No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC15350.251127620275406No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA14780.2418023601088274No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG14750.24131155694216538No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT14480.2368943284422071No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG14310.23411311049778893No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT14230.23280430205335684No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT14190.2321498978311408No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC13980.22871427566450658No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG13940.22805987144229053No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC13650.22331544083122423No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC13540.22151582922013013No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT13490.2206978239423601No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC13330.21808020705349593No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT12970.21219056905355155No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12300.20122929833143285No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT12000.19632126666481253No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA11840.19370364977594837No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT11800.19304924555373232No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA11250.18405118749826174No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC10930.1788159537205334No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA10880.17799794844276334No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA10830.1771799431649933No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTC10600.17341711888725106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10570.17292631572058903No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT10530.17227191149837298No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA10520.172108310442819No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC10460.1711267041094949No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG10290.16834548616507675No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA10260.16785468299841472No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA10060.1645826618873345No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT10040.16425545977622646No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT9910.16212864605402433No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT9860.1613106407762543No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC9810.16049263549848422No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG9580.156729811220742No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT9540.15607540699852596No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT9470.15493019960964788No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAA9420.1541121943318778No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGAT9120.14920416266525752No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA9060.14822255633193346No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAAT9040.14789535422082542No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA8930.14609574260973132No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAAAACTAT8870.14511413627640724No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA8730.1428237214986511No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTTACCAAAAACATC8700.14233291833198908No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC8550.13987890249867893No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA8490.13889729616535484No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC8460.13840649299869284No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG8420.1377520887764768No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA8120.13284405710985647No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA7900.12924483388766825No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC7870.12875403072100622No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT7810.12777242438768216No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC7780.12728162122102013No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT7770.1271180201654661No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT7700.12597281277658803No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC7670.125482009609926No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGT7660.12531840855437199No Hit
CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTTATCCCTAG7480.1223735895543998No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATCCT7480.1223735895543998No Hit
ATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAA7480.1223735895543998No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCC7460.12204638744329177No Hit
CGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTA7340.12008317477664365No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT7280.1191015684433196No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA7260.11877436633221158No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA7250.11861076527665756No Hit
GCTTTTAACCATTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTC7190.11762915894333349No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA7140.11681115366556345No Hit
TGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGGTCA7050.11533874416557736No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGAC7010.11468433994336132No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACC7000.11452073888780731No Hit
TCTTTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAA6970.11402993572114527No Hit
CAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGTA6950.11370273361003724No Hit
GGTTATAATCATTCGGAGGATTTTTTATTCTCCGAGGTCACCCCAACCGA6940.11353913255448324No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA6930.11337553149892922No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAGCATGAAC6920.11321193044337523No Hit
CTATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGA6900.1128847283322672No Hit
GCGGTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAG6830.11173952094338913No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6820.1115759198878351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.1115759198878351No Hit
GCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAAT6790.11108511672117309No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT6720.10993990933229501No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT6620.1083038987767549No Hit
GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACTTTTAGTAG6540.10699509033232281No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC6520.10666788822121481No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGA6480.10601348399899876No Hit
AACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGA6420.10503187766567469No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG6410.10486827661012067No Hit
CAAGAACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATTACAAGTAT6350.10388667027679663No Hit
TAATCACACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACC6270.10257786183236456No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG6250.10225065972125652No Hit
CTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGA6220.10175985655459449No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCAT6200.10143265444348647No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG6180.10110545233237844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAG207.8538014E-443.99975218
TCGTATA301.300425E-436.66646244
ATACATG800.035.7585751
GTGACCA250.002351949735.19980611
TCTGGAG250.002351949735.1998063
ATTGCGA300.00574297729.3331717
TAGGACC2400.028.4165084
GCTTACT953.092282E-1127.789328
TGGGGAG407.0283574E-427.4998466
GTATAGG655.9107333E-727.0834181
CTCTAGG508.321214E-526.4063341
AGGACCT4000.026.3998555
GGACCTG4000.025.8498576
TTACTAG901.5095793E-824.4443110
GGGCGTT450.001397469224.4443133
GTCGTAT450.001397469224.4443143
TAGGGCG450.001397469224.4443131
CAACCGT551.5924222E-423.9998666
TATAGGG551.5924222E-423.9998662
GATATAC2400.023.839051
Produced by FastQC (version 0.11.5)