FastQCFastQC Report
Thu 2 Feb 2017
SRR4062449_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062449_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740528
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG76200.43779818537823006No Hit
TCCATGTACTCTGCGTTGATACCAC61060.35081308660360533No Hit
GTATCAACGCAGAGTACTTTTTTTT32630.18747184762324998No Hit
GAGTACATGGAAGCAGTGGTATCAA30690.17632580458343675No Hit
CATGTACTCTGCGTTGATACCACTG25910.14886287379461866No Hit
TATCAACGCAGAGTACTTTTTTTTT23930.13748701543439693No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23440.13467177775939254No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG22920.1316841785940818No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21920.1259387955838688No Hit
GAATAGGACCGCGGTTCTATTTTGT21820.1253642572828475No Hit
GCGCAAGACGGACCAGAGCGAAAGC21620.1242151806808049No Hit
GTCCTATTCCATTATTCCTAGCTGC20510.11783780553946849No Hit
GAATAACGCCGCCGCATCGCCAGTC20110.11553965233538328No Hit
GATTAAGAGGGACGGCCGGGGGCAT20110.11553965233538328No Hit
GCTTCCATGTACTCTGCGTTGATAC19990.11485020637415774No Hit
GTATCTGATCGTCTTCGAACCTCCG19640.11283932232058318No Hit
GGGTAGGCACACGCTGAGCCAGTCA19470.11186260720884697No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG18710.1074961161210851No Hit
CTATTGGAGCTGGAATTACCGCGGC18570.10669176249965527No Hit
GAACTACGACGGTATCTGATCGTCT18360.10548523206751054No Hit
GTACATGGGAAGCAGTGGTATCAAC17910.10289980971291471No Hit
CCCATGTACTCTGCGTTGATACCAC17620.10123364863995293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2200.013.82144616
TCGCCAG3400.013.41609217
CGCCAGT3600.012.670753518
ACGAACG2400.012.66747315
CGCATCG3600.012.66710813
TCGCGTA1600.012.4691849
TCTATAC1155.351467E-912.3871033
GGTTCGT701.0877633E-412.21822618
AAGACGG4050.012.1965535
TTCCGAT2600.012.0581117
GCGTTAT1900.011.9945511
TAACGAA2550.011.92198413
ATTCCGA2550.011.9219846
ATAACGA2550.011.9216412
CGGTTCT4150.011.90368612
CGTCTTA1201.0006261E-811.87575615
ATCGCCA3850.011.84695416
CGATAAC2650.011.83060110
GATAACG2650.011.83026111
AATTCCG2650.011.829245
Produced by FastQC (version 0.11.5)