##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062449_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1740528
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14382934373937	32.0	32.0	32.0	32.0	32.0
2	30.703875490655708	32.0	32.0	32.0	32.0	32.0
3	30.706088037652943	32.0	32.0	32.0	32.0	32.0
4	30.792733584291664	32.0	32.0	32.0	32.0	32.0
5	30.518859794269325	32.0	32.0	32.0	32.0	32.0
6	34.20005596003052	36.0	36.0	36.0	32.0	36.0
7	34.066287931018636	36.0	36.0	36.0	32.0	36.0
8	34.035912091043635	36.0	36.0	36.0	32.0	36.0
9	34.258661739426195	36.0	36.0	36.0	32.0	36.0
10	33.83278062748775	36.0	36.0	36.0	32.0	36.0
11	34.251032445326935	36.0	36.0	36.0	32.0	36.0
12	33.971223100116745	36.0	36.0	36.0	32.0	36.0
13	34.13386742413796	36.0	36.0	36.0	32.0	36.0
14	34.00943908974748	36.0	36.0	36.0	32.0	36.0
15	33.92821086474909	36.0	36.0	36.0	32.0	36.0
16	33.928494686669794	36.0	36.0	36.0	32.0	36.0
17	33.82675601886324	36.0	36.0	36.0	32.0	36.0
18	33.85774776389693	36.0	36.0	36.0	32.0	36.0
19	33.849491073972956	36.0	36.0	36.0	32.0	36.0
20	33.84401687304082	36.0	36.0	36.0	32.0	36.0
21	33.81100677495564	36.0	36.0	36.0	32.0	36.0
22	33.76399173124477	36.0	36.0	36.0	32.0	36.0
23	33.70286717593741	36.0	36.0	36.0	27.0	36.0
24	33.673998349926	36.0	36.0	36.0	27.0	36.0
25	33.17947140178153	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	73.0
5	147.0
6	325.0
7	88.0
8	263.0
9	314.0
10	173.0
11	72.0
12	108.0
13	87.0
14	243.0
15	341.0
16	540.0
17	741.0
18	1064.0
19	1544.0
20	2440.0
21	3631.0
22	5742.0
23	8583.0
24	11962.0
25	16895.0
26	23647.0
27	30101.0
28	39992.0
29	53327.0
30	68809.0
31	93339.0
32	133016.0
33	187745.0
34	398081.0
35	657082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.56144850791549	16.894432493059004	10.79141155055436	25.752707448471142
2	17.025395112651704	19.71900419028839	36.36225741229438	26.89334328476552
3	19.12198759962331	23.736936425517587	28.127860282883955	29.013215691975148
4	12.322470688557448	14.941480546763847	35.54510893674337	37.19093982793534
5	14.621930942285996	36.52333141943794	33.428568308536036	15.42616932974004
6	35.07357505805532	34.83738762559492	16.673142804589244	13.415894511760513
7	30.736371267262296	30.229684822978832	20.71348902457254	18.320454885186336
8	28.981006911439383	32.000729154267304	19.454962935616482	19.563300998676826
9	27.23107535353419	14.123252457055443	18.357956604001448	40.28771558540892
10	15.64320555887902	26.627398647094015	31.55860291749114	26.170792876535824
11	37.95229165707975	20.969640145414385	21.50499286733238	19.573075330173484
12	24.274417261419188	23.76183512459025	28.56635781339709	23.39738980059347
13	29.59454142593858	19.068022796978504	25.298364774664705	26.03907100241821
14	23.675883257412032	19.569517890639965	24.67707801841094	32.07752083353706
15	25.64536306615399	26.800721272061633	22.57472269274903	24.979192969035342
16	26.387796877669118	25.511938374715363	23.597277637597728	24.50298711001779
17	24.322032728343704	25.66295374222288	25.258508817140964	24.756504712292458
18	25.50151986175126	24.171497012050743	26.341758345433163	23.98522478076483
19	25.28443100854573	25.76628247794534	25.053457554672825	23.895828958836102
20	25.264273835840463	23.98295486248327	25.5288784274204	25.22389287425587
21	26.74222276681215	24.452356338998026	23.927421502826594	24.877999391363225
22	25.465775967225944	24.558102603051854	24.691822596607516	25.284298833114686
23	24.043731880743575	24.208621069570466	25.247491998616912	26.500155051069047
24	24.37320112274678	25.692123686240713	24.798804765978403	25.135870425034103
25	25.020724235072944	24.475249711931692	24.76806402566181	25.735962027333553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	178.0
1	178.0
2	521.0
3	864.0
4	864.0
5	864.0
6	2345.0
7	3826.0
8	3826.0
9	3826.0
10	4039.0
11	4252.0
12	4252.0
13	4252.0
14	4481.0
15	4710.0
16	4710.0
17	4710.0
18	7763.5
19	10817.0
20	10817.0
21	10817.0
22	17447.5
23	24078.0
24	24078.0
25	24078.0
26	35599.0
27	47120.0
28	47120.0
29	47120.0
30	57336.0
31	67552.0
32	67552.0
33	67552.0
34	85493.5
35	103435.0
36	103435.0
37	103435.0
38	118000.0
39	132565.0
40	132565.0
41	132565.0
42	155383.0
43	178201.0
44	178201.0
45	178201.0
46	213179.5
47	248158.0
48	248158.0
49	248158.0
50	241657.0
51	235156.0
52	235156.0
53	235156.0
54	216304.5
55	197453.0
56	197453.0
57	197453.0
58	181856.5
59	166260.0
60	166260.0
61	166260.0
62	147922.5
63	129585.0
64	129585.0
65	129585.0
66	108554.5
67	87524.0
68	87524.0
69	87524.0
70	65760.0
71	43996.0
72	43996.0
73	43996.0
74	34767.0
75	25538.0
76	25538.0
77	25538.0
78	21295.5
79	17053.0
80	17053.0
81	17053.0
82	11948.0
83	6843.0
84	6843.0
85	6843.0
86	5002.5
87	3162.0
88	3162.0
89	3162.0
90	2171.0
91	1180.0
92	1180.0
93	1180.0
94	702.0
95	224.0
96	224.0
97	224.0
98	511.0
99	798.0
100	798.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00827335153470671
2	0.004309037257659744
3	0.005573021519906603
4	0.014478365185736742
5	0.03567882849342269
6	0.04642269472252098
7	0.06825515016133035
8	0.0877319985659524
9	0.09525845030933142
10	0.11766544404916209
11	0.11766544404916209
12	0.13042019433183494
13	0.11789525936957061
14	0.1209403123649835
15	0.11105825358741715
16	0.1086451927231277
17	0.12042322789406433
18	0.1270878721859114
19	0.12829440261805614
20	0.11979123576294089
21	0.12737514133642205
22	0.14754143570226966
23	0.13737210777419268
24	0.13162672476397966
25	0.1278347719772391
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1740528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.346808414412294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63131813980199	38.30857783203099
2	13.362458367756375	13.187893460384686
3	4.116969524549787	6.094779191277974
4	1.6777175671620954	3.311600294409673
5	0.854161530216155	2.107507269326892
6	0.5142463769237821	1.5225850463918114
7	0.33579918665281605	1.1599432690630394
8	0.23403263824499093	0.9239011009756021
9	0.17707401068471407	0.7864233552387164
>10	0.8953516524728321	8.055763690479845
>50	0.08557056404691028	2.983799259196329
>100	0.09291684401528857	10.054623944332333
>500	0.014456073367496176	4.832100870669878
>1k	0.0076943616310866745	5.880856720758121
>5k	2.3316247366929316E-4	0.7896446954641373
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7620	0.43779818537823006	No Hit
TCCATGTACTCTGCGTTGATACCAC	6106	0.35081308660360533	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3263	0.18747184762324998	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3069	0.17632580458343675	No Hit
CATGTACTCTGCGTTGATACCACTG	2591	0.14886287379461866	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2393	0.13748701543439693	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2344	0.13467177775939254	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2292	0.1316841785940818	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2192	0.1259387955838688	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2182	0.1253642572828475	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2162	0.1242151806808049	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2051	0.11783780553946849	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2011	0.11553965233538328	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2011	0.11553965233538328	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1999	0.11485020637415774	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1964	0.11283932232058318	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1947	0.11186260720884697	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1871	0.1074961161210851	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1857	0.10669176249965527	No Hit
GAACTACGACGGTATCTGATCGTCT	1836	0.10548523206751054	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1791	0.10289980971291471	No Hit
CCCATGTACTCTGCGTTGATACCAC	1762	0.10123364863995293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.745383010212993E-5
2	0.0	0.0	0.0	5.745383010212993E-5	5.745383010212993E-5
3	0.0	0.0	0.0	5.745383010212993E-5	5.745383010212993E-5
4	0.0	0.0	0.0	5.745383010212993E-5	5.745383010212993E-5
5	0.0	0.0	0.0	1.1490766020425986E-4	5.745383010212993E-5
6	0.0	0.0	0.0	1.1490766020425986E-4	5.745383010212993E-5
7	0.0	0.0	0.0	1.7236149030638978E-4	5.745383010212993E-5
8	0.0	0.0	0.0	1.7236149030638978E-4	5.745383010212993E-5
9	0.0	0.0	0.0	2.2981532040851972E-4	5.745383010212993E-5
10	0.0	0.0	0.0	2.2981532040851972E-4	1.7236149030638978E-4
11	0.0	0.0	5.745383010212993E-5	2.2981532040851972E-4	1.7236149030638978E-4
12	0.0	0.0	5.745383010212993E-5	2.2981532040851972E-4	5.170844709191693E-4
13	0.0	0.0	5.745383010212993E-5	2.2981532040851972E-4	5.745383010212992E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	220	0.0	13.821446	16
TCGCCAG	340	0.0	13.416092	17
CGCCAGT	360	0.0	12.6707535	18
ACGAACG	240	0.0	12.667473	15
CGCATCG	360	0.0	12.667108	13
TCGCGTA	160	0.0	12.469184	9
TCTATAC	115	5.351467E-9	12.387103	3
GGTTCGT	70	1.0877633E-4	12.218226	18
AAGACGG	405	0.0	12.196553	5
TTCCGAT	260	0.0	12.058111	7
GCGTTAT	190	0.0	11.994551	1
TAACGAA	255	0.0	11.921984	13
ATTCCGA	255	0.0	11.921984	6
ATAACGA	255	0.0	11.92164	12
CGGTTCT	415	0.0	11.903686	12
CGTCTTA	120	1.0006261E-8	11.875756	15
ATCGCCA	385	0.0	11.846954	16
CGATAAC	265	0.0	11.830601	10
GATAACG	265	0.0	11.830261	11
AATTCCG	265	0.0	11.82924	5
>>END_MODULE