FastQCFastQC Report
Thu 2 Feb 2017
SRR4062449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740528
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA29930.1719593134956749No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27020.15524024893595506No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC25450.14621999760992066No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23150.13300561668643077No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22090.126915510695605No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21740.12490462664203046No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19110.1097942693251703No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19030.10933463868435325No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA18710.1074961161210851No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG18580.1067492163297574No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT18200.10456597078587647No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC18120.10410634014505944No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA18030.10358925567414026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA959.640644E-1025.4734532
CGATTAA1001.7462298E-924.1997833
CGTCTTA2850.020.84191315
AATACGG550.00448462819.9998175
TCGTATA901.00245325E-519.55537844
ATACGGT600.00741377218.3331646
ATCGTTT6000.017.96650329
GGCGTTA5450.017.76130542
TACTATA1751.9099389E-1017.6013572
TTAGGAC1651.6607373E-917.3336733
CGCAATA6400.017.18734436
AACGAAT902.2162302E-417.11095631
CATCGTT6500.016.92292228
TAGCGGC6650.016.54120330
ATACGAA6700.016.4177640
CAATACG6850.016.37941438
CTATTAC1351.0858948E-616.2966163
ATACCGT5550.016.2521046
TCTCGTC3250.016.24600612
CTAGGAC2450.016.1635823
Produced by FastQC (version 0.11.5)