##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062449_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1740528
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251944812148956	32.0	32.0	32.0	32.0	32.0
2	31.393562183429395	32.0	32.0	32.0	32.0	32.0
3	31.49423967899396	32.0	32.0	32.0	32.0	32.0
4	31.598231686017115	32.0	32.0	32.0	32.0	32.0
5	31.531754157359146	32.0	32.0	32.0	32.0	32.0
6	35.130148437715455	36.0	36.0	36.0	36.0	36.0
7	35.14154497945451	36.0	36.0	36.0	36.0	36.0
8	35.08722065947804	36.0	36.0	36.0	36.0	36.0
9	35.217020926983075	36.0	36.0	36.0	36.0	36.0
10	35.087109199047646	36.0	36.0	36.0	36.0	36.0
11	35.225536159142514	36.0	36.0	36.0	36.0	36.0
12	35.130411576257316	36.0	36.0	36.0	36.0	36.0
13	35.18065552521993	36.0	36.0	36.0	36.0	36.0
14	35.13924510263552	36.0	36.0	36.0	36.0	36.0
15	35.095386572350456	36.0	36.0	36.0	36.0	36.0
16	35.10298139415166	36.0	36.0	36.0	36.0	36.0
17	35.07655033415148	36.0	36.0	36.0	36.0	36.0
18	35.090113459823684	36.0	36.0	36.0	36.0	36.0
19	35.06842119173032	36.0	36.0	36.0	36.0	36.0
20	35.062387390493	36.0	36.0	36.0	36.0	36.0
21	35.04738447183843	36.0	36.0	36.0	36.0	36.0
22	35.03107103131923	36.0	36.0	36.0	36.0	36.0
23	34.98343261355175	36.0	36.0	36.0	36.0	36.0
24	34.95693720526185	36.0	36.0	36.0	32.0	36.0
25	34.91902342277746	36.0	36.0	36.0	32.0	36.0
26	34.882633315867366	36.0	36.0	36.0	32.0	36.0
27	34.855948309938135	36.0	36.0	36.0	32.0	36.0
28	34.83652604267211	36.0	36.0	36.0	32.0	36.0
29	34.80245821957475	36.0	36.0	36.0	32.0	36.0
30	34.7922463758124	36.0	36.0	36.0	32.0	36.0
31	34.784016114650264	36.0	36.0	36.0	32.0	36.0
32	34.75203788735372	36.0	36.0	36.0	32.0	36.0
33	34.72109382899902	36.0	36.0	36.0	32.0	36.0
34	34.701677307116	36.0	36.0	36.0	32.0	36.0
35	34.65958548210658	36.0	36.0	36.0	32.0	36.0
36	34.63061266466268	36.0	36.0	36.0	32.0	36.0
37	34.610116010773744	36.0	36.0	36.0	32.0	36.0
38	34.5539502955425	36.0	36.0	36.0	32.0	36.0
39	34.510686986937294	36.0	36.0	36.0	32.0	36.0
40	34.47361030675749	36.0	36.0	36.0	32.0	36.0
41	34.458289093884154	36.0	36.0	36.0	32.0	36.0
42	34.38358417675556	36.0	36.0	36.0	32.0	36.0
43	34.40095132051883	36.0	36.0	36.0	32.0	36.0
44	34.370446783964404	36.0	36.0	36.0	32.0	36.0
45	34.281805291267936	36.0	36.0	36.0	32.0	36.0
46	34.332809354402805	36.0	36.0	36.0	32.0	36.0
47	34.27567611667264	36.0	36.0	36.0	32.0	36.0
48	34.25660948861495	36.0	36.0	36.0	32.0	36.0
49	34.26635882904498	36.0	36.0	36.0	32.0	36.0
50	33.723468395797134	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	6.0
21	38.0
22	159.0
23	458.0
24	1217.0
25	2903.0
26	6232.0
27	11710.0
28	19593.0
29	29276.0
30	41375.0
31	58963.0
32	87363.0
33	137902.0
34	304331.0
35	1039000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87780715429645	17.501024283418214	11.314180047476183	26.306988514809156
2	16.385054968289957	20.15980851017376	36.03517005294009	27.419966468596197
3	18.524804440065154	23.88287374713806	28.49231405647179	29.100007756325
4	12.11720601542638	15.33663693425834	35.75375245974634	36.792404590568935
5	14.311921439930872	36.6503153066196	33.48478162948255	15.55298162396698
6	35.25697190668771	35.02692584576687	16.368087486448026	13.348014761097396
7	30.536480883961648	30.285982184716364	20.816097184302695	18.361439747019297
8	28.85051481534041	32.299182948612696	19.363732938357174	19.486569297689723
9	27.257935523013703	13.891761580393997	18.213668495996618	40.63663440059568
10	15.578893301343042	26.59635466938768	31.673721997003206	26.15103003226607
11	38.1744505115689	20.809719809161358	21.499970124008346	19.515859555261393
12	24.213227250581433	23.662647196712722	28.684226855299084	23.439898697406765
13	29.7933730454207	18.8847866854196	25.359028984308207	25.962811284851494
14	23.657533805833634	19.42680611860309	24.8058635080849	32.10979656747838
15	25.731674526350623	26.7263726869088	22.468756607190464	25.073196179550113
16	26.51344879255031	25.43946434645119	23.542741053289575	24.504345807708923
17	24.373465982736274	25.686400908230144	25.21091301030492	24.72922009872866
18	25.707141740897015	24.012828291185205	26.343385455448004	23.93664451246978
19	25.324614140077035	25.685998731419428	24.97971879797395	24.00966833052959
20	25.406083671161856	23.844718384306372	25.509730380666095	25.239467563865674
21	26.77273792780122	24.39627515328682	23.968071757535643	24.862915161376318
22	25.460090271457858	24.43810154160117	24.75047801586645	25.351330171074526
23	24.117624077291488	23.989616943823943	25.3075503525367	26.585208626347868
24	24.46343868067621	25.457332487612955	24.877968064863076	25.20126076684776
25	25.207580688159	24.26493569767335	24.72381943869906	25.803664175468594
26	24.615059338315728	25.04240092661537	25.375173510566906	24.96736622450199
27	25.848765432098762	24.712673395659248	24.595467582250905	24.843093589991085
28	24.685440280190836	24.0829219639098	25.6204439112729	25.611193844626456
29	24.366399161633712	24.967021501521376	25.77849939788386	24.88807993896105
30	24.957541619554526	24.687795887225025	25.829633306674754	24.52502918654569
31	24.795694180156826	24.844529935743637	24.62695228114687	25.732823602952664
32	24.609773585946336	24.76340512763943	24.709915611814345	25.916905674599892
33	24.2987185497734	24.305268286405045	25.235618157248833	26.160395006572717
34	24.951394059733598	24.78316924519456	25.657788900839286	24.607647794232555
35	26.096219078348636	24.28573398417032	25.278995798975945	24.3390511385051
36	24.66205657133927	25.19666446043959	24.9380073173198	25.20327165090134
37	26.023252714118932	24.76696726510576	24.846713181287516	24.363066839487786
38	24.674868775452047	24.570130443175863	25.107955746761903	25.647045034610187
39	25.215854039693703	24.534394160852337	24.76018771315371	25.48956408630025
40	25.62745327854536	24.777596223674657	24.96420626384637	24.630744233933612
41	24.06424946912661	25.164949946223214	25.75149609773586	25.01930448691432
42	26.26800603035401	25.135591039041028	24.925654743847844	23.67074818675712
43	25.174200012869658	23.75411369423531	25.051076454960793	26.020609837934238
44	24.743985733064907	24.670042653723467	25.226597905922798	25.359373707288825
45	24.863030069036522	24.86975216715847	25.111230615077723	25.15598714872728
46	24.413870052001492	24.63334380908771	25.6166092223792	25.3361769165316
47	24.949498083340227	24.21575521910593	25.933222562348895	24.90152413520495
48	25.612917459529523	25.77068567698997	24.24339051138505	24.373006352095455
49	24.281482400742764	25.161675077907393	25.381780701028656	25.175061820321186
50	24.27961432348612	25.88263547915975	24.47851971185722	25.359230485496916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	11.5
2	16.0
3	65.0
4	114.0
5	157.5
6	201.0
7	190.0
8	179.0
9	267.0
10	355.0
11	527.0
12	699.0
13	1251.5
14	1804.0
15	2563.5
16	3323.0
17	4095.5
18	4868.0
19	5400.5
20	5933.0
21	6912.5
22	7892.0
23	8500.0
24	9108.0
25	10152.5
26	11197.0
27	13794.0
28	16391.0
29	18717.0
30	21043.0
31	22822.5
32	24602.0
33	28161.0
34	31720.0
35	36131.5
36	40543.0
37	49900.5
38	59258.0
39	65361.0
40	71464.0
41	79796.5
42	88129.0
43	91798.5
44	95468.0
45	108347.5
46	121227.0
47	131358.5
48	141490.0
49	142489.5
50	143489.0
51	134272.0
52	125055.0
53	120974.0
54	116893.0
55	119050.0
56	121207.0
57	118248.5
58	115290.0
59	105474.0
60	95658.0
61	84546.5
62	73435.0
63	64159.5
64	54884.0
65	47109.0
66	39334.0
67	34456.5
68	29579.0
69	27471.0
70	25363.0
71	19621.5
72	13880.0
73	12907.5
74	11935.0
75	8569.0
76	5203.0
77	4772.5
78	4342.0
79	3716.5
80	3091.0
81	2515.5
82	1940.0
83	1687.0
84	1434.0
85	1163.0
86	892.0
87	655.5
88	419.0
89	268.0
90	117.0
91	75.5
92	34.0
93	22.0
94	10.0
95	6.0
96	2.0
97	4.5
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01648924923931129
2	0.004021768107149095
3	7.46899791327689E-4
4	1.7236149030638978E-4
5	0.0
6	4.0217681071490956E-4
7	0.0
8	5.7453830102129924E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	5.7453830102129924E-5
47	0.0
48	0.0
49	0.0
50	1.1490766020425985E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1740528.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.157111362211005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70469940216663	40.52852660085935
2	13.542603830382035	14.126861922310823
3	4.085160257698271	6.39210475479742
4	1.6506956622926283	3.44382069933261
5	0.8213589749851513	2.141985576332601
6	0.47904288650633386	1.4991295907271516
7	0.3154628170030809	1.1517540493946534
8	0.21626704143146966	0.9023891331133651
9	0.16255951372951472	0.763077119451945
>10	0.840726592872346	7.949361811269491
>50	0.07880181046079579	2.8810817095430807
>100	0.08457711928242553	9.536474189794353
>500	0.01261979383793026	4.475726073708724
>1k	0.005424297351391077	4.207706769364496
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2993	0.1719593134956749	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2702	0.15524024893595506	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2545	0.14621999760992066	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2315	0.13300561668643077	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2209	0.126915510695605	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2174	0.12490462664203046	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1911	0.1097942693251703	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1903	0.10933463868435325	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1871	0.1074961161210851	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1858	0.1067492163297574	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1820	0.10456597078587647	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1812	0.10410634014505944	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1803	0.10358925567414026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.745383010212993E-5	0.0
3	0.0	0.0	0.0	5.745383010212993E-5	0.0
4	0.0	0.0	0.0	5.745383010212993E-5	0.0
5	0.0	0.0	0.0	1.1490766020425986E-4	0.0
6	0.0	0.0	0.0	1.1490766020425986E-4	1.1490766020425986E-4
7	0.0	0.0	0.0	1.1490766020425986E-4	1.1490766020425986E-4
8	0.0	0.0	0.0	1.1490766020425986E-4	1.1490766020425986E-4
9	0.0	0.0	0.0	1.1490766020425986E-4	1.1490766020425986E-4
10	0.0	0.0	0.0	1.1490766020425986E-4	1.1490766020425986E-4
11	0.0	0.0	0.0	1.1490766020425986E-4	1.1490766020425986E-4
12	0.0	0.0	0.0	1.1490766020425986E-4	1.7236149030638978E-4
13	0.0	0.0	0.0	1.1490766020425986E-4	2.872691505106496E-4
14	0.0	0.0	0.0	1.1490766020425986E-4	2.872691505106496E-4
15	0.0	0.0	0.0	1.7236149030638978E-4	5.170844709191693E-4
16	0.0	0.0	0.0	2.2981532040851972E-4	5.745383010212992E-4
17	0.0	0.0	0.0	3.4472298061277956E-4	6.319921311234292E-4
18	0.0	0.0	0.0	5.170844709191693E-4	6.319921311234292E-4
19	0.0	0.0	0.0	5.745383010212992E-4	6.319921311234292E-4
20	0.0	0.0	0.0	6.319921311234292E-4	8.04353621429819E-4
21	0.0	0.0	0.0	9.767151117362088E-4	0.0010341689418383387
22	0.0	0.0	0.0	0.0014937995826553782	0.0010341689418383387
23	0.0	0.0	0.0	0.002527968524493717	0.0010341689418383387
24	0.0	0.0	0.0	0.0036195912964341855	0.0010341689418383387
25	0.0	0.0	0.0	0.004653760238272524	0.0010341689418383387
26	0.0	0.0	0.0	0.005975198330621513	0.0010341689418383387
27	0.0	0.0	0.0	0.00741154408317476	0.0010341689418383387
28	0.0	0.0	0.0	0.012352573471957935	0.0010341689418383387
29	0.0	0.0	0.0	0.021257917137788074	0.0010341689418383387
30	0.0	0.0	0.0	0.03596609764393333	0.0010341689418383387
31	0.0	0.0	0.0	0.06687625823887923	0.0010916227719404686
32	0.0	0.0	0.0	0.1063470395190425	0.0010916227719404686
33	0.0	0.0	0.0	0.1447836518573674	0.0010916227719404686
34	0.0	0.0	0.0	0.18781657060386273	0.0012639842622468585
35	0.0	0.0	0.0	0.23791631045292003	0.0012639842622468585
36	0.0	0.0	0.0	0.3133531893770166	0.0012639842622468585
37	0.0	0.0	0.0	0.4307888181057702	0.0012639842622468585
38	0.0	0.0	0.0	0.5751128393223206	0.0012639842622468585
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	95	9.640644E-10	25.47345	32
CGATTAA	100	1.7462298E-9	24.19978	33
CGTCTTA	285	0.0	20.841913	15
AATACGG	55	0.004484628	19.999817	5
TCGTATA	90	1.00245325E-5	19.555378	44
ATACGGT	60	0.007413772	18.333164	6
ATCGTTT	600	0.0	17.966503	29
GGCGTTA	545	0.0	17.761305	42
TACTATA	175	1.9099389E-10	17.601357	2
TTAGGAC	165	1.6607373E-9	17.333673	3
CGCAATA	640	0.0	17.187344	36
AACGAAT	90	2.2162302E-4	17.110956	31
CATCGTT	650	0.0	16.922922	28
TAGCGGC	665	0.0	16.541203	30
ATACGAA	670	0.0	16.41776	40
CAATACG	685	0.0	16.379414	38
CTATTAC	135	1.0858948E-6	16.296616	3
ATACCGT	555	0.0	16.252104	6
TCTCGTC	325	0.0	16.246006	12
CTAGGAC	245	0.0	16.163582	3
>>END_MODULE