FastQCFastQC Report
Thu 2 Feb 2017
SRR4062448_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062448_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085865
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38320.18371275226344946No Hit
TATCAACGCAGAGTACTTTTTTTTT26430.12671002198128833No Hit
GATTAAGAGGGACGGCCGGGGGCAT22230.10657449067892698No Hit
GAATAACGCCGCCGCATCGCCAGTC22100.10595124804337769No Hit
GCGCAAGACGGACCAGAGCGAAAGC21350.10235561745367029No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21340.10230767571247419No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21140.10134884088855223No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21020.10077353999419904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1950.013.64333616
CGTCTTA1650.012.09208615
ACGGTAT4000.011.87539
TTAGACT1201.00371835E-811.87358954
GATAACG2250.011.82280511
CGATAAC2250.011.82252110
CGTTATT1701.8189894E-1211.7310852
ACGAACG2200.011.66022615
CCGATAA2400.011.4794589
ATAACGA2500.011.4011112
CGTCGTA3850.011.35093610
TAACGAA2450.011.24572213
GTATTAG4500.011.1813871
CGAGCCG4450.011.10220315
AAGACGG4200.011.0822835
ATCGCCA4500.010.97963716
CGCCAGT4250.010.95611218
ATTATAC1752.0008883E-1110.8537693
TTATACG1053.4823915E-610.8532492
TTAGGAC2200.010.79213
Produced by FastQC (version 0.11.5)