##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062448_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2085865
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180357309797134	32.0	32.0	32.0	32.0	32.0
2	30.848045774774494	32.0	32.0	32.0	32.0	32.0
3	30.83437326960278	32.0	32.0	32.0	32.0	32.0
4	30.900777375333494	32.0	32.0	32.0	32.0	32.0
5	30.73259007653899	32.0	32.0	32.0	32.0	32.0
6	34.36756837091566	36.0	36.0	36.0	32.0	36.0
7	34.251916590958665	36.0	36.0	36.0	32.0	36.0
8	34.26049720379795	36.0	36.0	36.0	32.0	36.0
9	34.3936510752134	36.0	36.0	36.0	32.0	36.0
10	34.09118039758086	36.0	36.0	36.0	32.0	36.0
11	34.38024320845309	36.0	36.0	36.0	32.0	36.0
12	34.16403937934622	36.0	36.0	36.0	32.0	36.0
13	34.28202208676017	36.0	36.0	36.0	32.0	36.0
14	34.187989155578144	36.0	36.0	36.0	32.0	36.0
15	34.14458174426437	36.0	36.0	36.0	32.0	36.0
16	34.141358141586345	36.0	36.0	36.0	32.0	36.0
17	34.067901326308274	36.0	36.0	36.0	32.0	36.0
18	34.073659608843336	36.0	36.0	36.0	32.0	36.0
19	34.063394802635834	36.0	36.0	36.0	32.0	36.0
20	34.05732058402629	36.0	36.0	36.0	32.0	36.0
21	34.033589901551636	36.0	36.0	36.0	32.0	36.0
22	33.992173990167146	36.0	36.0	36.0	32.0	36.0
23	33.96467029266036	36.0	36.0	36.0	32.0	36.0
24	33.933580552912105	36.0	36.0	36.0	32.0	36.0
25	33.496520148715284	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	69.0
5	200.0
6	367.0
7	100.0
8	299.0
9	382.0
10	261.0
11	80.0
12	193.0
13	118.0
14	360.0
15	461.0
16	715.0
17	979.0
18	1302.0
19	1903.0
20	2895.0
21	4218.0
22	6394.0
23	9364.0
24	12917.0
25	18276.0
26	24915.0
27	32000.0
28	43237.0
29	56545.0
30	71565.0
31	97246.0
32	138258.0
33	198639.0
34	454765.0
35	906831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22425930134547	17.510186194721495	10.891197344157694	26.374357159775343
2	17.53250707050658	20.07927980795646	35.99598614808774	26.392226973449223
3	19.05598185797537	23.64226075324881	28.341831414373143	28.95992597440268
4	13.025180670205911	15.268166753613405	35.44564358486513	36.261008991315556
5	15.152064494625048	36.00961088850362	33.17291206564563	15.6654125512257
6	34.282536343347694	35.07748786876345	16.771395558650816	13.868580229238042
7	30.402589838873872	30.268118173871912	20.780309855722408	18.548982131531808
8	28.4281100150722	31.843275979633095	19.690504033409326	20.03810997188538
9	27.72692022712178	14.02700622319821	18.66983457272619	39.57623897695382
10	16.38972222355556	26.099273758838017	31.091185745897192	26.419818271709232
11	37.4612997151659	21.060203003094102	22.108530718786092	19.369966562953902
12	24.85165882874804	23.7550383190689	28.228364752217438	23.164938099965624
13	29.39494464783466	19.283760308779783	25.18349520015629	26.137799843229264
14	23.667534711805015	19.79474132654898	24.686195682175473	31.851528279470532
15	25.515209578079162	26.812881103414384	21.971763788839908	25.70014552966655
16	26.114884160711117	25.55198249091668	23.34779624572232	24.98533710264988
17	24.368621653017925	25.483290290991828	24.9057228713242	25.242365184666042
18	25.12093899643556	24.578567742400057	25.870551948441605	24.42994131272278
19	25.8430995646722	24.827030649612006	25.082908151018614	24.246961634697183
20	25.821675795731043	24.19591671374803	24.651353903209035	25.331053587311885
21	27.107785083666208	23.878365838589524	24.032655193056115	24.98119388468815
22	26.246631698430654	24.16001329074661	24.423043312084783	25.170311698737958
23	24.78398951454353	23.998540485912567	25.18852457048757	26.028945429056332
24	24.87706332696906	24.95714079279572	24.85253120164687	25.313264678588354
25	24.991058814041097	24.46044443334964	24.94689295588167	25.601603796727595
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	259.0
1	259.0
2	675.5
3	1092.0
4	1092.0
5	1092.0
6	2735.0
7	4378.0
8	4378.0
9	4378.0
10	4756.5
11	5135.0
12	5135.0
13	5135.0
14	5946.0
15	6757.0
16	6757.0
17	6757.0
18	10766.0
19	14775.0
20	14775.0
21	14775.0
22	23291.5
23	31808.0
24	31808.0
25	31808.0
26	46678.0
27	61548.0
28	61548.0
29	61548.0
30	74970.5
31	88393.0
32	88393.0
33	88393.0
34	108504.5
35	128616.0
36	128616.0
37	128616.0
38	144937.5
39	161259.0
40	161259.0
41	161259.0
42	183480.0
43	205701.0
44	205701.0
45	205701.0
46	232940.5
47	260180.0
48	260180.0
49	260180.0
50	264770.5
51	269361.0
52	269361.0
53	269361.0
54	251716.0
55	234071.0
56	234071.0
57	234071.0
58	217222.0
59	200373.0
60	200373.0
61	200373.0
62	180712.0
63	161051.0
64	161051.0
65	161051.0
66	136551.5
67	112052.0
68	112052.0
69	112052.0
70	86830.0
71	61608.0
72	61608.0
73	61608.0
74	49057.5
75	36507.0
76	36507.0
77	36507.0
78	29697.5
79	22888.0
80	22888.0
81	22888.0
82	16641.5
83	10395.0
84	10395.0
85	10395.0
86	7656.5
87	4918.0
88	4918.0
89	4918.0
90	3270.0
91	1622.0
92	1622.0
93	1622.0
94	931.5
95	241.0
96	241.0
97	241.0
98	559.0
99	877.0
100	877.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009540406498023601
2	0.004218873225256668
3	0.004794174119609851
4	0.014238697135241256
5	0.0349974710731519
6	0.045928188065862366
7	0.06764579682769498
8	0.09089754130780275
9	0.09823262771080582
10	0.12153231393210968
11	0.12210761482646289
12	0.13562718584376265
13	0.12311439139158094
14	0.12656619675770003
15	0.11702579025967644
16	0.11386163534073394
17	0.12680590546368053
18	0.1337574579371148
19	0.13601071977333146
20	0.12426499318028732
21	0.13260685614840845
22	0.15403681446306447
23	0.14286638876437355
24	0.1381680981271559
25	0.1346204092786446
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2085865.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.76392900298651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4830816220309	40.34873512399358
2	12.44037418283436	12.630445435759793
3	3.4558485423354206	5.262973501445691
4	1.4109757590596204	2.8650669303135023
5	0.8052270946748872	2.0438245532678536
6	0.48995585207618975	1.4923250453636072
7	0.3247036951538835	1.153826472945938
8	0.2310120196943875	0.9381662213281926
9	0.17013003060703952	0.7772821915510508
>10	0.9705635202503822	9.008003364274412
>50	0.09849034219249934	3.535235744689765
>100	0.10051992537348621	11.07863381850955
>500	0.014101458632869848	4.94187986553289
>1k	0.005015955084175181	3.923601731024178
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3832	0.18371275226344946	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2643	0.12671002198128833	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2223	0.10657449067892698	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2210	0.10595124804337769	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2135	0.10235561745367029	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2134	0.10230767571247419	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2114	0.10134884088855223	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2102	0.10077353999419904	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.91766964784394E-4
2	0.0	0.0	0.0	0.0	1.91766964784394E-4
3	0.0	0.0	0.0	0.0	1.91766964784394E-4
4	0.0	0.0	0.0	0.0	1.91766964784394E-4
5	0.0	0.0	0.0	0.0	1.91766964784394E-4
6	0.0	0.0	0.0	0.0	1.91766964784394E-4
7	0.0	0.0	0.0	0.0	1.91766964784394E-4
8	0.0	0.0	0.0	0.0	1.91766964784394E-4
9	0.0	0.0	0.0	4.79417411960985E-5	1.91766964784394E-4
10	0.0	0.0	0.0	4.79417411960985E-5	1.91766964784394E-4
11	0.0	0.0	0.0	1.91766964784394E-4	1.91766964784394E-4
12	0.0	0.0	0.0	1.91766964784394E-4	4.314756707648865E-4
13	0.0	0.0	0.0	1.91766964784394E-4	4.79417411960985E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	195	0.0	13.643336	16
CGTCTTA	165	0.0	12.092086	15
ACGGTAT	400	0.0	11.8753	9
TTAGACT	120	1.00371835E-8	11.8735895	4
GATAACG	225	0.0	11.822805	11
CGATAAC	225	0.0	11.822521	10
CGTTATT	170	1.8189894E-12	11.731085	2
ACGAACG	220	0.0	11.660226	15
CCGATAA	240	0.0	11.479458	9
ATAACGA	250	0.0	11.40111	12
CGTCGTA	385	0.0	11.350936	10
TAACGAA	245	0.0	11.245722	13
GTATTAG	450	0.0	11.181387	1
CGAGCCG	445	0.0	11.102203	15
AAGACGG	420	0.0	11.082283	5
ATCGCCA	450	0.0	10.979637	16
CGCCAGT	425	0.0	10.956112	18
ATTATAC	175	2.0008883E-11	10.853769	3
TTATACG	105	3.4823915E-6	10.853249	2
TTAGGAC	220	0.0	10.7921	3
>>END_MODULE