FastQCFastQC Report
Thu 2 Feb 2017
SRR4062448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085865
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22270.10676625764371135No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC22130.10609507326696599No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG21340.10230767571247419No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21180.10154060785333661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTA450.001398659824.44412615
GTATCAA39750.020.0970481
TAGTACG1404.083631E-918.8582522
TAATACC1852.1827873E-1117.838464
TAGGACG2900.017.4488854
TTAGGAC3950.017.2664243
GTATTAG6150.017.1762711
GTCCTAA3700.016.654021
AACGCAG48900.016.3314676
TAATACT4450.015.8207774
GTAATAC1552.6688758E-715.6134463
GTCCTAT6700.015.109351
GGTATCA22900.014.99173451
TCAACGC54300.014.6671784
TACGACG6150.014.6668275
TCCTACA6350.014.5520372
CAACGCA55000.014.4801585
TATCAAC56200.014.4065422
CCTATTC8150.014.3072463
ATCAACG56150.014.1839323
Produced by FastQC (version 0.11.5)