##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062448_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2085865
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.305755166321887	32.0	32.0	32.0	32.0	32.0
2	31.437980885627784	32.0	32.0	32.0	32.0	32.0
3	31.505563878774513	32.0	32.0	32.0	32.0	32.0
4	31.6046695255925	32.0	32.0	32.0	32.0	32.0
5	31.544915898200507	32.0	32.0	32.0	32.0	32.0
6	35.129034237594475	36.0	36.0	36.0	36.0	36.0
7	35.144445589719375	36.0	36.0	36.0	36.0	36.0
8	35.08768352697802	36.0	36.0	36.0	36.0	36.0
9	35.212142204792734	36.0	36.0	36.0	36.0	36.0
10	35.08461046136735	36.0	36.0	36.0	36.0	36.0
11	35.21194947899313	36.0	36.0	36.0	36.0	36.0
12	35.123201165943144	36.0	36.0	36.0	36.0	36.0
13	35.16939830717712	36.0	36.0	36.0	36.0	36.0
14	35.130227028115435	36.0	36.0	36.0	36.0	36.0
15	35.092897191333094	36.0	36.0	36.0	36.0	36.0
16	35.10922327188001	36.0	36.0	36.0	36.0	36.0
17	35.077675688503334	36.0	36.0	36.0	36.0	36.0
18	35.092555846135774	36.0	36.0	36.0	36.0	36.0
19	35.073924726672146	36.0	36.0	36.0	36.0	36.0
20	35.061817519350484	36.0	36.0	36.0	36.0	36.0
21	35.06917370011961	36.0	36.0	36.0	36.0	36.0
22	35.046606084286374	36.0	36.0	36.0	36.0	36.0
23	35.0159324788517	36.0	36.0	36.0	36.0	36.0
24	34.98714346326344	36.0	36.0	36.0	36.0	36.0
25	34.96962938636968	36.0	36.0	36.0	32.0	36.0
26	34.92230082004348	36.0	36.0	36.0	32.0	36.0
27	34.90833922617236	36.0	36.0	36.0	32.0	36.0
28	34.900110985130866	36.0	36.0	36.0	32.0	36.0
29	34.87526709542564	36.0	36.0	36.0	32.0	36.0
30	34.856852193214806	36.0	36.0	36.0	32.0	36.0
31	34.86482442535831	36.0	36.0	36.0	32.0	36.0
32	34.82202827124478	36.0	36.0	36.0	32.0	36.0
33	34.807912304967005	36.0	36.0	36.0	32.0	36.0
34	34.7898713483375	36.0	36.0	36.0	32.0	36.0
35	34.77042186335166	36.0	36.0	36.0	32.0	36.0
36	34.74021952523294	36.0	36.0	36.0	32.0	36.0
37	34.73750554326383	36.0	36.0	36.0	32.0	36.0
38	34.710274634264444	36.0	36.0	36.0	32.0	36.0
39	34.70957612309522	36.0	36.0	36.0	32.0	36.0
40	34.6941762769882	36.0	36.0	36.0	32.0	36.0
41	34.65944296490904	36.0	36.0	36.0	32.0	36.0
42	34.64413516694513	36.0	36.0	36.0	32.0	36.0
43	34.63165017870284	36.0	36.0	36.0	32.0	36.0
44	34.602302162412236	36.0	36.0	36.0	32.0	36.0
45	34.565910545505105	36.0	36.0	36.0	32.0	36.0
46	34.56830619431267	36.0	36.0	36.0	32.0	36.0
47	34.54078811428352	36.0	36.0	36.0	32.0	36.0
48	34.500058249215556	36.0	36.0	36.0	32.0	36.0
49	34.48167259146685	36.0	36.0	36.0	32.0	36.0
50	33.96381261491036	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	0.0
20	18.0
21	49.0
22	199.0
23	516.0
24	1448.0
25	3292.0
26	6716.0
27	12364.0
28	20291.0
29	31644.0
30	45000.0
31	65004.0
32	95245.0
33	157772.0
34	348701.0
35	1297604.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.661715874899606	18.120665060356504	11.444120784934249	26.773498279809637
2	16.907872089689487	20.576773897843196	35.72726479474766	26.78808921771966
3	18.437915597160686	23.960881250557325	28.76131311203948	28.839890040242512
4	12.693661127784223	15.734613061279282	35.60066351528866	35.97106229564784
5	14.771425763412301	36.24956552797041	33.11920953657116	15.859799172046129
6	34.43067868669493	35.31564080289493	16.506588195135606	13.747092315274525
7	30.260118004028076	30.41559336887741	20.774011307560762	18.550277319533755
8	28.4406708967263	32.13395881325014	19.49138606765059	19.93398422237297
9	27.77907486822014	13.798352242355092	18.56428867640044	39.858284213024334
10	16.34070277798419	26.21564674607417	31.101341649627372	26.342308826314266
11	37.63666392599713	20.937740457795687	22.10382742890839	19.321768187298794
12	24.780127189439394	23.703259798692628	28.236487020972113	23.280125990895865
13	29.524921315617263	19.184942457925132	25.188734649653743	26.101401576803866
14	23.813909337373225	19.558456563583935	24.68812698808408	31.939507110958765
15	25.612060224415288	26.782270185270857	21.928360656130668	25.67730893418318
16	26.25198658590081	25.449202129572146	23.345566467628537	24.953244816898504
17	24.49928446951265	25.439469956109335	24.86397729479137	25.19726827958665
18	25.312328458457284	24.378998640851638	25.880677800337033	24.42799510035405
19	25.952782179095962	24.7002083068655	25.06849676273393	24.278512751304614
20	25.977616001035543	24.03549606518159	24.571436790012775	25.415451143770092
21	27.278371323168088	23.85130389550618	24.010278709312445	24.86004607201329
22	26.251842760677224	24.04757738396301	24.50268833313757	25.197891522222193
23	24.881332205104357	23.7982323880021	25.210068724486007	26.110366682407538
24	25.08949524537782	24.78544872271216	24.76430641484468	25.36074961706534
25	25.23399165334286	24.183108686324378	24.973572115165652	25.609327545167115
26	24.854772480481717	24.915179074388803	25.404136892847813	24.825911552281667
27	25.780575444719577	24.403688637567626	24.52128972872166	25.294446188991138
28	24.811672855146426	24.438158749487624	25.436257859449196	25.313910535916754
29	24.86201168340233	24.641623499123867	25.156757508275945	25.339607309197866
30	24.866470265333568	24.747095329755282	25.463920244119347	24.922514160791806
31	25.388124351288315	24.46251315401524	24.43930935127633	25.710053143420115
32	25.08412577036385	24.45465070845908	24.56611525674001	25.895108264437056
33	24.878263933667807	24.04963887883444	25.10234363201837	25.96975355547938
34	25.349914783555022	24.251521551011212	25.374365071565038	25.02419859386873
35	26.046843875322708	24.115319064273095	25.06940765581665	24.768429404587543
36	24.826678620140804	24.630548956907568	24.876346264019965	25.666426158931664
37	25.888875838081564	24.381012193981874	24.46524583326342	25.26486613467315
38	24.858607819777408	24.18191014279448	25.35461307419224	25.604868963235877
39	25.45735222557548	24.045228238644402	24.591572321315137	25.905847214464984
40	25.637661114214005	24.221414137540062	25.402075397976382	24.73884935026955
41	24.532747804867526	24.57555977975564	25.65281070443197	25.23888171094486
42	25.771850047821886	24.678970115515625	25.06677086005087	24.482408976611623
43	24.924671539145628	23.75263979212461	25.440093198744883	25.882595469984874
44	24.58246339048788	24.29174467187474	25.17363300117697	25.952158936460414
45	24.568656169023402	24.541185551318037	25.426046268574424	25.46411201108413
46	24.50050554711512	24.14894774766655	25.489834164246002	25.86071254097233
47	24.599578592094886	24.00356686554499	25.89673828363772	25.5001162587224
48	25.169605894916497	24.735109894456254	25.039971426722246	25.055312783905
49	24.216236429490884	24.665067010568755	25.401260388376045	25.717436171564316
50	24.03429752165169	25.058524880565137	25.24012819621596	25.667049401567215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	23.5
2	31.0
3	109.0
4	187.0
5	232.5
6	278.0
7	271.5
8	265.0
9	395.0
10	525.0
11	734.5
12	944.0
13	1722.5
14	2501.0
15	3311.5
16	4122.0
17	5014.0
18	5906.0
19	6389.0
20	6872.0
21	7953.0
22	9034.0
23	9572.5
24	10111.0
25	12425.0
26	14739.0
27	18354.5
28	21970.0
29	25362.5
30	28755.0
31	30322.0
32	31889.0
33	37180.0
34	42471.0
35	48793.0
36	55115.0
37	66267.0
38	77419.0
39	82951.5
40	88484.0
41	97272.5
42	106061.0
43	106809.5
44	107558.0
45	117504.5
46	127451.0
47	135962.5
48	144474.0
49	149199.0
50	153924.0
51	149059.0
52	144194.0
53	141656.5
54	139119.0
55	141583.0
56	144047.0
57	141991.5
58	139936.0
59	128716.5
60	117497.0
61	104188.5
62	90880.0
63	80951.5
64	71023.0
65	61221.0
66	51419.0
67	46996.0
68	42573.0
69	39307.5
70	36042.0
71	28821.0
72	21600.0
73	19424.0
74	17248.0
75	13240.0
76	9232.0
77	8251.0
78	7270.0
79	6257.0
80	5244.0
81	4088.0
82	2932.0
83	2599.0
84	2266.0
85	1782.0
86	1298.0
87	984.5
88	671.0
89	428.5
90	186.0
91	115.5
92	45.0
93	32.0
94	19.0
95	13.5
96	8.0
97	7.5
98	7.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01630019200667349
2	0.004362698448844964
3	5.273591531570835E-4
4	2.3970870598049248E-4
5	0.0
6	6.232426355492805E-4
7	1.91766964784394E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.8765044717659103E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2085865.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.18383063349608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22278154169945	43.467776080033985
2	12.179822037386055	13.198988288396984
3	3.3348910837203953	5.420915209843864
4	1.3914286268630078	3.0157173222617297
5	0.7020415665047746	1.901965066858449
6	0.4409703715748526	1.4336078356680986
7	0.29504732723065885	1.1190756085272202
8	0.22198878524726218	0.9622562193898552
9	0.16232288794803962	0.7915748281664886
>10	0.8670390457993593	8.578727438663568
>50	0.08236047998174754	3.168147312985435
>100	0.08366144103108458	9.667925369269712
>500	0.012000276572781837	4.3699182873137135
>1k	0.0036445284406226306	2.9034051326208585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2227	0.10676625764371135	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2213	0.10609507326696599	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2134	0.10230767571247419	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2118	0.10154060785333661	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.91766964784394E-4
2	0.0	0.0	0.0	0.0	1.91766964784394E-4
3	0.0	0.0	0.0	0.0	1.91766964784394E-4
4	0.0	0.0	0.0	0.0	1.91766964784394E-4
5	0.0	0.0	0.0	0.0	1.91766964784394E-4
6	0.0	0.0	0.0	0.0	2.397087059804925E-4
7	0.0	0.0	0.0	0.0	2.397087059804925E-4
8	0.0	0.0	0.0	0.0	2.397087059804925E-4
9	0.0	0.0	0.0	4.79417411960985E-5	3.355921883726895E-4
10	0.0	0.0	0.0	4.79417411960985E-5	3.355921883726895E-4
11	0.0	0.0	0.0	1.4382522358829551E-4	3.355921883726895E-4
12	0.0	0.0	0.0	1.4382522358829551E-4	6.71184376745379E-4
13	0.0	0.0	0.0	1.91766964784394E-4	6.71184376745379E-4
14	0.0	0.0	0.0	1.91766964784394E-4	6.71184376745379E-4
15	0.0	0.0	0.0	2.397087059804925E-4	8.150096003336745E-4
16	0.0	0.0	0.0	2.397087059804925E-4	9.108930827258715E-4
17	0.0	0.0	0.0	2.397087059804925E-4	9.108930827258715E-4
18	0.0	0.0	0.0	2.397087059804925E-4	0.001054718306314167
19	0.0	0.0	0.0	4.314756707648865E-4	0.0011026600475102656
20	0.0	0.0	0.0	6.232426355492805E-4	0.001246485271098561
21	0.0	0.0	0.0	7.191261179414775E-4	0.0012944270122946596
22	0.0	0.0	0.0	9.5883482392197E-4	0.0012944270122946596
23	0.0	0.0	0.0	0.0011026600475102656	0.0012944270122946596
24	0.0	0.0	0.0	0.001821786165451743	0.0013903104946868564
25	0.0	0.0	0.0	0.0020614948714322355	0.0013903104946868564
26	0.0	0.0	0.0	0.003068271436550304	0.001534135718275152
27	0.0	0.0	0.0	0.004746232378413751	0.0015820774594712506
28	0.0	0.0	0.0	0.012321027487397315	0.0015820774594712506
29	4.79417411960985E-5	0.0	0.0	0.02977182128277717	0.0015820774594712506
30	4.79417411960985E-5	0.0	0.0	0.063762515790811	0.0015820774594712506
31	4.79417411960985E-5	0.0	0.0	0.138216039868352	0.001630019200667349
32	4.79417411960985E-5	0.0	0.0	0.2276273871990757	0.001630019200667349
33	4.79417411960985E-5	0.0	0.0	0.32801739326370594	0.001630019200667349
34	4.79417411960985E-5	0.0	0.0	0.4353589518017705	0.001630019200667349
35	4.79417411960985E-5	0.0	0.0	0.5537750525561338	0.001630019200667349
36	4.79417411960985E-5	0.0	0.0	0.7281871070275401	0.001630019200667349
37	4.79417411960985E-5	0.0	0.0	0.9767170933881147	0.001630019200667349
38	4.79417411960985E-5	0.0	0.0	1.3040633022750754	0.001725902683059546
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGCTA	45	0.0013986598	24.444126	15
GTATCAA	3975	0.0	20.097048	1
TAGTACG	140	4.083631E-9	18.858252	2
TAATACC	185	2.1827873E-11	17.83846	4
TAGGACG	290	0.0	17.448885	4
TTAGGAC	395	0.0	17.266424	3
GTATTAG	615	0.0	17.176271	1
GTCCTAA	370	0.0	16.65402	1
AACGCAG	4890	0.0	16.331467	6
TAATACT	445	0.0	15.820777	4
GTAATAC	155	2.6688758E-7	15.613446	3
GTCCTAT	670	0.0	15.10935	1
GGTATCA	2290	0.0	14.9917345	1
TCAACGC	5430	0.0	14.667178	4
TACGACG	615	0.0	14.666827	5
TCCTACA	635	0.0	14.552037	2
CAACGCA	5500	0.0	14.480158	5
TATCAAC	5620	0.0	14.406542	2
CCTATTC	815	0.0	14.307246	3
ATCAACG	5615	0.0	14.183932	3
>>END_MODULE