FastQCFastQC Report
Thu 2 Feb 2017
SRR4062447_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062447_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004282
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG109591.09122736442553No Hit
TCCATGTACTCTGCGTTGATACCAC89260.8887941833070792No Hit
GAGTACATGGAAGCAGTGGTATCAA46400.4620216234085645No Hit
CATGTACTCTGCGTTGATACCACTG38110.37947508767457744No Hit
GTATCAACGCAGAGTACTTTTTTTT33530.3338703670881287No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30350.30220595410452444No Hit
GCTTCCATGTACTCTGCGTTGATAC29090.2896596772619643No Hit
CATGGAAGCAGTGGTATCAACGCAG26120.26008631041878677No Hit
TATCAACGCAGAGTACTTTTTTTTT24190.2408686006520081No Hit
GCGTTGATACCACTGCTTCCATGTA24000.2389767017630506No Hit
CCCATGTACTCTGCGTTGATACCAC23380.23280313696750513No Hit
GTACATGGGAAGCAGTGGTATCAAC21840.21746879860437607No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19110.19028519877882905No Hit
GTATCAACGCAGAGTACATGGAAGC19100.19018562515309445No Hit
ACTCTGCGTTGATACCACTGCTTCC16330.16260373082460902No Hit
ACGCAGAGTACATGGAAGCAGTGGT15890.15822249129228644No Hit
CAGTGGTATCAACGCAGAGTACATG14410.14348559468356498No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13740.1368141617593465No Hit
GTACATGGGGTGGTATCAACGCAAA12340.12287385415650186No Hit
TATCAACGCAGAGTACATGGAAGCA11340.11291649158304141No Hit
GGTATCAACGCAGAGTACTTTTTTT10870.10823653117351502No Hit
GTATCAACGCAGAGTACATGGGAAG10600.1055480432786807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACT307.7220285E-418.9993024
AATACCG350.002169287816.2859295
GTAAACG500.001497626213.30150117
ACGATCA655.442959E-513.1553329
GACGATC655.4455064E-513.15467558
TATACTG957.3750925E-813.0001715
CGAACGA751.4756322E-512.66809716
CGATAAC751.4756322E-512.66809710
GCGTTAT751.4847366E-512.660521
CGAGCCG1600.012.47077815
CCGACCT701.08951564E-412.21505519
CGTTATT701.094598E-412.2089672
ACGCACG550.0030645512.0922749
TTTCGTT550.0030645512.09227417
AACCGCG550.003065694812.0916717
GGTTCCG550.003065694812.0916718
GTACGCA550.003065694812.0916717
AGTACGC550.003066839912.0910676
GTCCTAT1650.012.0850431
CGTCTTA802.8592214E-511.87693215
Produced by FastQC (version 0.11.5)