##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062447_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1004282
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.022051575155185	32.0	32.0	32.0	32.0	32.0
2	30.7023176757126	32.0	32.0	32.0	32.0	32.0
3	30.70564542628465	32.0	32.0	32.0	32.0	32.0
4	30.74784672034349	32.0	32.0	32.0	32.0	32.0
5	30.563706210008743	32.0	32.0	32.0	32.0	32.0
6	34.162123785948566	36.0	36.0	36.0	32.0	36.0
7	34.01156049794779	36.0	36.0	36.0	32.0	36.0
8	34.00213884148078	36.0	36.0	36.0	32.0	36.0
9	34.156929029893995	36.0	36.0	36.0	32.0	36.0
10	33.8382784914994	36.0	36.0	36.0	32.0	36.0
11	34.19262418324733	36.0	36.0	36.0	32.0	36.0
12	33.96060170350559	36.0	36.0	36.0	32.0	36.0
13	34.07527069090156	36.0	36.0	36.0	32.0	36.0
14	33.96952449610767	36.0	36.0	36.0	32.0	36.0
15	33.923373116315936	36.0	36.0	36.0	32.0	36.0
16	33.90011769602562	36.0	36.0	36.0	32.0	36.0
17	33.823278720518736	36.0	36.0	36.0	32.0	36.0
18	33.84016142876204	36.0	36.0	36.0	32.0	36.0
19	33.834756572357165	36.0	36.0	36.0	32.0	36.0
20	33.81514056808745	36.0	36.0	36.0	32.0	36.0
21	33.73539902139041	36.0	36.0	36.0	32.0	36.0
22	33.69235732593037	36.0	36.0	36.0	27.0	36.0
23	33.62895182827134	36.0	36.0	36.0	27.0	36.0
24	33.61793898526509	36.0	36.0	36.0	27.0	36.0
25	33.06489711057253	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	50.0
5	87.0
6	171.0
7	52.0
8	138.0
9	198.0
10	121.0
11	31.0
12	75.0
13	47.0
14	241.0
15	339.0
16	537.0
17	710.0
18	976.0
19	1276.0
20	1867.0
21	2753.0
22	3880.0
23	5297.0
24	7321.0
25	9901.0
26	13378.0
27	17371.0
28	22801.0
29	30313.0
30	39059.0
31	52964.0
32	75659.0
33	107521.0
34	227418.0
35	381725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.64321158666347	17.03494063368549	11.161643873812599	25.16020390583844
2	15.640820358398955	20.87819294912392	37.25807672239018	26.22290997008695
3	19.28832611050921	25.374119718309856	28.23354152061691	27.10401265056402
4	12.247952699210359	15.810848627713428	35.61680942749273	36.324389245583475
5	13.727484353723879	37.453843111903126	33.920469204004725	14.898203330368268
6	33.70544853563071	34.7814479020453	17.75403935601423	13.759064206309754
7	29.183240334794743	31.14936229573535	21.490035870864887	18.17736149860502
8	28.33942286034353	32.64195773808456	20.527685919937174	18.490933481634745
9	27.325280646341255	14.720689239307132	18.581613441123324	39.37241667322829
10	15.321894059779043	27.503504158134728	32.67430569816987	24.50029608391636
11	36.72134906511542	21.54685887754034	21.801578162930618	19.930213894413622
12	23.53992765443226	24.413039750180467	29.761187839244478	22.285844756142794
13	30.109145577949164	20.13311214438959	25.337067226019478	24.42067505164177
14	23.77028096754447	19.9084976182263	25.404422980676628	30.9167984335526
15	25.191274970417798	27.119625734072216	24.216831798647643	23.472267496862344
16	25.506282740780247	25.924168490098644	25.135050266910564	23.43449850221055
17	23.316933108017167	26.030497440268785	27.039435313822548	23.6131341378915
18	24.774144661079898	24.328864915525468	28.450743545387922	22.446246878006708
19	24.32731279070347	27.358256352478428	25.68978668035954	22.624644176458563
20	24.24262967789018	24.28440256300079	28.034790133623382	23.438177625485647
21	25.235077993748533	25.610066153853822	24.403643197918175	24.751212654479467
22	23.918673518333662	26.329388605397234	25.37563336866868	24.376304507600423
23	22.79979618555889	25.699565149325988	25.645420706310766	25.855217958804356
24	22.99447218544103	27.28740248552239	25.57282277491505	24.145302554121532
25	24.608638517686916	25.222812578204994	25.20686000520453	24.96168889890356
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	274.0
1	274.0
2	635.5
3	997.0
4	997.0
5	997.0
6	2694.0
7	4391.0
8	4391.0
9	4391.0
10	4198.0
11	4005.0
12	4005.0
13	4005.0
14	3794.0
15	3583.0
16	3583.0
17	3583.0
18	5798.5
19	8014.0
20	8014.0
21	8014.0
22	12557.5
23	17101.0
24	17101.0
25	17101.0
26	25022.5
27	32944.0
28	32944.0
29	32944.0
30	40648.5
31	48353.0
32	48353.0
33	48353.0
34	56717.5
35	65082.0
36	65082.0
37	65082.0
38	72387.0
39	79692.0
40	79692.0
41	79692.0
42	93794.5
43	107897.0
44	107897.0
45	107897.0
46	137211.0
47	166525.0
48	166525.0
49	166525.0
50	149973.5
51	133422.0
52	133422.0
53	133422.0
54	116664.5
55	99907.0
56	99907.0
57	99907.0
58	90722.5
59	81538.0
60	81538.0
61	81538.0
62	72048.5
63	62559.0
64	62559.0
65	62559.0
66	51926.5
67	41294.0
68	41294.0
69	41294.0
70	31465.5
71	21637.0
72	21637.0
73	21637.0
74	16763.0
75	11889.0
76	11889.0
77	11889.0
78	9756.0
79	7623.0
80	7623.0
81	7623.0
82	5365.5
83	3108.0
84	3108.0
85	3108.0
86	2241.5
87	1375.0
88	1375.0
89	1375.0
90	959.0
91	543.0
92	543.0
93	543.0
94	307.0
95	71.0
96	71.0
97	71.0
98	264.5
99	458.0
100	458.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00926034719331821
2	0.0037837977779149683
3	0.0057752702926070565
4	0.015433911988863687
5	0.03674266789606903
6	0.046899177720998685
7	0.06790921275100022
8	0.09160773567583606
9	0.09688513783977011
10	0.11829346737271007
11	0.1211811025190136
12	0.13123803871820863
13	0.1212806761447482
14	0.12426788491678634
15	0.11401180146612207
16	0.11321521246024523
17	0.12416831129105173
18	0.131138465092474
19	0.13213420134982007
20	0.12337172228517489
21	0.131138465092474
22	0.15025660123351808
23	0.1402992386600576
24	0.13482268924465438
25	0.1303418760865972
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1004282.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.27772213388192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.23591238769963	41.42273010828773
2	12.839086803711153	13.423964247544035
3	3.711983962427438	5.821621984596229
4	1.4723068877985797	3.078754015045384
5	0.7348424731488242	1.920794531172441
6	0.43533981365919283	1.3655144287374732
7	0.2667188387530832	0.9760417338143739
8	0.17971198763985402	0.7515946683171129
9	0.15000274154233487	0.705762147750362
>10	0.7693568090568724	7.685664990485607
>50	0.0963728263381473	3.5501551640169984
>100	0.09541405735254753	9.839859030105897
>500	0.008760572060679804	3.0896910305212044
>1k	0.00380894437420861	4.388072132220268
>5k	1.9044721871043052E-4	0.8886856616644305
>10k+	1.9044721871043052E-4	1.0910941257204227
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10959	1.09122736442553	No Hit
TCCATGTACTCTGCGTTGATACCAC	8926	0.8887941833070792	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4640	0.4620216234085645	No Hit
CATGTACTCTGCGTTGATACCACTG	3811	0.37947508767457744	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3353	0.3338703670881287	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3035	0.30220595410452444	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2909	0.2896596772619643	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2612	0.26008631041878677	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2419	0.2408686006520081	No Hit
GCGTTGATACCACTGCTTCCATGTA	2400	0.2389767017630506	No Hit
CCCATGTACTCTGCGTTGATACCAC	2338	0.23280313696750513	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2184	0.21746879860437607	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1911	0.19028519877882905	No Hit
GTATCAACGCAGAGTACATGGAAGC	1910	0.19018562515309445	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1633	0.16260373082460902	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1589	0.15822249129228644	No Hit
CAGTGGTATCAACGCAGAGTACATG	1441	0.14348559468356498	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1374	0.1368141617593465	No Hit
GTACATGGGGTGGTATCAACGCAAA	1234	0.12287385415650186	No Hit
TATCAACGCAGAGTACATGGAAGCA	1134	0.11291649158304141	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1087	0.10823653117351502	No Hit
GTATCAACGCAGAGTACATGGGAAG	1060	0.1055480432786807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	9.957362573460442E-5	0.0
12	0.0	0.0	0.0	9.957362573460442E-5	1.9914725146920884E-4
13	0.0	0.0	0.0	9.957362573460442E-5	1.9914725146920884E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATACT	30	7.7220285E-4	18.999302	4
AATACCG	35	0.0021692878	16.285929	5
GTAAACG	50	0.0014976262	13.301501	17
ACGATCA	65	5.442959E-5	13.155332	9
GACGATC	65	5.4455064E-5	13.1546755	8
TATACTG	95	7.3750925E-8	13.000171	5
CGAACGA	75	1.4756322E-5	12.668097	16
CGATAAC	75	1.4756322E-5	12.668097	10
GCGTTAT	75	1.4847366E-5	12.66052	1
CGAGCCG	160	0.0	12.470778	15
CCGACCT	70	1.08951564E-4	12.215055	19
CGTTATT	70	1.094598E-4	12.208967	2
ACGCACG	55	0.00306455	12.092274	9
TTTCGTT	55	0.00306455	12.092274	17
AACCGCG	55	0.0030656948	12.091671	7
GGTTCCG	55	0.0030656948	12.091671	8
GTACGCA	55	0.0030656948	12.091671	7
AGTACGC	55	0.0030668399	12.091067	6
GTCCTAT	165	0.0	12.085043	1
CGTCTTA	80	2.8592214E-5	11.876932	15
>>END_MODULE