FastQCFastQC Report
Thu 2 Feb 2017
SRR4062447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004282
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42890.4270712807757183No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39010.38843671399069185No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA16990.1691755901230929No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC15520.15453826714010607No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12210.12157939702195199No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10680.10634463228455751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGA250.002352669135.199844
TCGTATA502.3610319E-630.79982644
CGCAATA2550.019.84302536
TAGGACG3650.019.2875614
GTCGTAT808.98703E-519.24989143
TAATACT1851.8189894E-1219.0269184
ACGATTA708.122326E-418.85703540
CTAGCGG2700.018.74063529
GTATAGA1303.5775884E-818.6189881
AATCCCG1303.5857738E-818.6152819
CAATACG2750.018.39989738
CGTAAAT1203.1608397E-718.333233
CGCCGGT3000.018.333237
TCTAGCG3050.018.03268428
GTCCTAA2600.017.772671
TACGACG2500.017.5999015
ACGACGG2500.017.5999016
CGATTAA750.001290713117.599941
CGGTCCA3050.017.31137710
CGACGGT2550.017.2548057
Produced by FastQC (version 0.11.5)