##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1004282
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13759282751259	32.0	32.0	32.0	32.0	32.0
2	31.331351154357044	32.0	32.0	32.0	32.0	32.0
3	31.434609999980086	32.0	32.0	32.0	32.0	32.0
4	31.56284788535491	32.0	32.0	32.0	32.0	32.0
5	31.485346745236896	32.0	32.0	32.0	32.0	32.0
6	35.057472901037755	36.0	36.0	36.0	36.0	36.0
7	35.07778890789639	36.0	36.0	36.0	36.0	36.0
8	35.02790052993084	36.0	36.0	36.0	36.0	36.0
9	35.14091062072207	36.0	36.0	36.0	36.0	36.0
10	35.019616004269714	36.0	36.0	36.0	36.0	36.0
11	35.154986348455914	36.0	36.0	36.0	36.0	36.0
12	35.056512015549416	36.0	36.0	36.0	36.0	36.0
13	35.103243909579184	36.0	36.0	36.0	36.0	36.0
14	35.05416406945459	36.0	36.0	36.0	36.0	36.0
15	35.02363579154062	36.0	36.0	36.0	36.0	36.0
16	35.01778484529246	36.0	36.0	36.0	36.0	36.0
17	34.98962144098968	36.0	36.0	36.0	36.0	36.0
18	35.00281494639952	36.0	36.0	36.0	36.0	36.0
19	34.96622860909585	36.0	36.0	36.0	36.0	36.0
20	34.956216481028235	36.0	36.0	36.0	36.0	36.0
21	34.91412870090274	36.0	36.0	36.0	32.0	36.0
22	34.895758362690955	36.0	36.0	36.0	32.0	36.0
23	34.82646408080599	36.0	36.0	36.0	32.0	36.0
24	34.80172600922848	36.0	36.0	36.0	32.0	36.0
25	34.78205225225584	36.0	36.0	36.0	32.0	36.0
26	34.70647686605953	36.0	36.0	36.0	32.0	36.0
27	34.67723507939005	36.0	36.0	36.0	32.0	36.0
28	34.639159120645395	36.0	36.0	36.0	32.0	36.0
29	34.602014175301356	36.0	36.0	36.0	32.0	36.0
30	34.57648449339926	36.0	36.0	36.0	32.0	36.0
31	34.5611860015414	36.0	36.0	36.0	32.0	36.0
32	34.50612676519145	36.0	36.0	36.0	32.0	36.0
33	34.461464011104454	36.0	36.0	36.0	32.0	36.0
34	34.427217654005545	36.0	36.0	36.0	32.0	36.0
35	34.35096018847296	36.0	36.0	36.0	32.0	36.0
36	34.308280941010594	36.0	36.0	36.0	32.0	36.0
37	34.23750201636592	36.0	36.0	36.0	32.0	36.0
38	34.17333478047003	36.0	36.0	36.0	32.0	36.0
39	34.049827638053856	36.0	36.0	36.0	32.0	36.0
40	33.97223289872765	36.0	36.0	36.0	32.0	36.0
41	33.962656903140754	36.0	36.0	36.0	32.0	36.0
42	33.81763190020333	36.0	36.0	36.0	32.0	36.0
43	33.86197900589675	36.0	36.0	36.0	32.0	36.0
44	33.82744687249199	36.0	36.0	36.0	32.0	36.0
45	33.64237335728411	36.0	36.0	36.0	27.0	36.0
46	33.74691072826158	36.0	36.0	36.0	32.0	36.0
47	33.64039781654954	36.0	36.0	36.0	27.0	36.0
48	33.63229152767848	36.0	36.0	36.0	27.0	36.0
49	33.66884600142191	36.0	36.0	36.0	27.0	36.0
50	33.055815000169275	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	8.0
21	34.0
22	107.0
23	382.0
24	1072.0
25	2561.0
26	5341.0
27	10278.0
28	16354.0
29	23079.0
30	31459.0
31	42549.0
32	61115.0
33	85990.0
34	176483.0
35	547469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.85144590527672	17.60859476609975	11.810433940881108	25.72952538774242
2	15.093856765742952	21.293451883657642	36.8651810368606	26.747510313738804
3	18.695831408224066	25.550548058693263	28.630988417480523	27.122632115602148
4	12.067762046204292	16.134161921139444	35.855175703166154	35.942900329490115
5	13.452994278499467	37.63325440463933	33.8809218924565	15.032829424404698
6	34.09368132497309	34.99562371736084	17.246934859605467	13.663760098060596
7	29.020932367601926	31.00623131749847	21.62699321505314	18.345843099846455
8	28.405069492433398	32.73273841411078	20.33183906512314	18.53035302833268
9	27.26146640087147	14.300465407126683	18.435160642130395	40.00290754987145
10	15.250497370260543	27.50970344982784	32.6976884978522	24.54211068205942
11	36.86305240958217	21.182197828896665	21.92163157360184	20.033118187919328
12	23.482846451494698	24.388866872053867	29.760764406810043	22.367522269641395
13	30.325546011976716	19.82451144200533	25.445741335600957	24.404201210416996
14	23.704596916005663	19.614012797202378	25.455698698174416	31.225691588617536
15	25.402924676535076	26.914253167934906	24.133261374793136	23.549560780736883
16	25.64618304420472	25.60973909718585	25.251473191792744	23.492604666816693
17	23.523671638045887	25.908957842518337	26.97967304004254	23.58769747939324
18	25.006920366988556	24.021041898590237	28.41761576927596	22.55442196514525
19	24.440346436558656	27.052461360454533	25.81794754859691	22.689244654389903
20	24.283916270529595	24.044342127012133	28.122579116224326	23.549162486233946
21	25.411786729225454	25.445940482852425	24.43526818164619	24.707004606275927
22	23.99385829876469	25.866838198832596	25.623281110285756	24.516022392116955
23	22.95650026586158	25.13029208927373	25.87012412848184	26.043083516382847
24	23.197966308267997	26.990227844370406	25.633537193736423	24.178268653625175
25	24.895995347920206	24.720845340253035	25.253464664307433	25.129694647519322
26	22.93489278907717	25.464262029987594	26.58157768435559	25.019267496579644
27	25.161757355005864	25.8331823133343	25.8173501068425	23.18771022481733
28	23.847982937063495	24.49670510872444	26.435204454525717	25.220107499686346
29	23.129360080136856	26.054733630593795	27.15273200156928	23.663174287700066
30	24.773121493763703	24.96012076289329	26.957766842380927	23.308990900962083
31	23.38377069388877	25.31739093202905	26.11109230275958	25.187746071322596
32	23.316857217395114	25.884761451464826	26.196028605511202	24.602352725628858
33	23.009373861126655	25.206764633837906	26.488675491545205	25.295186013490234
34	23.099587566042207	26.189855040715653	27.42665904596518	23.28389834727696
35	25.147319179274348	25.3733513096919	26.44336949183596	23.035960019197795
36	23.69195106553737	26.200608992294995	26.212259106505943	23.895180835661698
37	24.984815022075473	25.812371425555767	26.108304241239015	23.094509311129745
38	23.834540497589323	25.82760619029316	25.50528636379025	24.832566948327262
39	23.754383728872966	25.584248248997792	26.32467772996031	24.33669029216893
40	24.92288022686855	25.683224432977987	25.630450411338646	23.763444928814813
41	22.977311153640112	25.72823171181003	26.91465146243784	24.379805672112017
42	25.560450152447224	26.08699548533181	25.968901165210568	22.3836531970104
43	24.18543795467807	24.615197723348622	25.729924463447517	25.469439858525796
44	23.684483043607273	25.397049832616737	27.1441686697561	23.77429845401989
45	24.28929324631926	25.670777729761163	25.95874465538564	24.081184368533936
46	23.378493291724833	25.21960963155767	27.026074349634865	24.37582272708263
47	24.337885175677748	24.74534045218375	27.26833698104716	23.64843739109135
48	23.89199447963819	27.523842904682148	24.874288297510063	23.709874318169597
49	22.992844639254713	25.559653563441344	27.567555726379645	23.8799460709243
50	22.879031985040058	27.74360189667842	24.58662009276279	24.79074602551873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	32.5
2	43.0
3	163.0
4	283.0
5	315.5
6	348.0
7	317.5
8	287.0
9	372.5
10	458.0
11	679.5
12	901.0
13	1579.0
14	2257.0
15	2988.5
16	3720.0
17	4354.5
18	4989.0
19	5378.5
20	5768.0
21	6543.5
22	7319.0
23	7796.0
24	8273.0
25	8462.5
26	8652.0
27	10359.0
28	12066.0
29	13887.0
30	15708.0
31	17389.5
32	19071.0
33	22066.0
34	25061.0
35	28577.0
36	32093.0
37	36509.5
38	40926.0
39	42773.0
40	44620.0
41	48185.0
42	51750.0
43	54213.5
44	56677.0
45	65789.5
46	74902.0
47	82170.5
48	89439.0
49	86444.0
50	83449.0
51	74985.5
52	66522.0
53	62916.0
54	59310.0
55	59100.5
56	58891.0
57	57108.5
58	55326.0
59	50743.5
60	46161.0
61	40894.5
62	35628.0
63	31194.5
64	26761.0
65	23013.0
66	19265.0
67	16736.0
68	14207.0
69	13166.0
70	12125.0
71	9598.0
72	7071.0
73	6405.0
74	5739.0
75	4204.0
76	2669.0
77	2355.0
78	2041.0
79	1771.5
80	1502.0
81	1179.0
82	856.0
83	694.5
84	533.0
85	449.0
86	365.0
87	261.0
88	157.0
89	97.0
90	37.0
91	26.0
92	15.0
93	13.0
94	11.0
95	6.5
96	2.0
97	3.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013542013099906202
2	0.003883371403649573
3	9.957362573460443E-4
4	2.9872087720381326E-4
5	0.0
6	4.978681286730222E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1004282.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.53167048828841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.73115181685917	45.8706635397924
2	12.692462239269023	14.604371104693373
3	3.615301105612524	6.239829357721336
4	1.410482961053285	3.245897637786517
5	0.6845972923794538	1.9693012921174586
6	0.3877817108876121	1.3385837767302495
7	0.28233027075601363	1.1370052474202956
8	0.17766626574868818	0.8177149646350569
9	0.13812295184550885	0.7151799737200989
>10	0.7087934161848969	7.750955005494366
>50	0.08130980698957774	3.2861281157110565
>100	0.08286281901762613	9.20727598589254
>500	0.006092854119082253	2.4420067420614897
>1k	0.0010444892775569577	1.3750872562237728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4289	0.4270712807757183	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3901	0.38843671399069185	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1699	0.1691755901230929	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1552	0.15453826714010607	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1221	0.12157939702195199	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1068	0.10634463228455751	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.9914725146920884E-4	0.0
8	0.0	0.0	0.0	1.9914725146920884E-4	0.0
9	0.0	0.0	0.0	1.9914725146920884E-4	0.0
10	0.0	0.0	0.0	1.9914725146920884E-4	0.0
11	0.0	0.0	0.0	2.9872087720381326E-4	0.0
12	0.0	0.0	0.0	2.9872087720381326E-4	0.0
13	0.0	0.0	0.0	6.97015380142231E-4	9.957362573460442E-5
14	0.0	0.0	0.0	6.97015380142231E-4	9.957362573460442E-5
15	0.0	0.0	0.0	8.961626316114398E-4	9.957362573460442E-5
16	0.0	0.0	0.0	0.0012944571345498575	9.957362573460442E-5
17	0.0	0.0	0.0	0.0014936043860190663	9.957362573460442E-5
18	0.0	0.0	0.0	0.002190619766161297	9.957362573460442E-5
19	0.0	0.0	0.0	0.002290193391895902	9.957362573460442E-5
20	0.0	0.0	0.0	0.0029872087720381325	1.9914725146920884E-4
21	0.0	0.0	0.0	0.0031863560235073415	2.9872087720381326E-4
22	0.0	0.0	0.0	0.004381239532322594	2.9872087720381326E-4
23	0.0	0.0	0.0	0.008165037310237563	2.9872087720381326E-4
24	0.0	0.0	0.0	0.012944571345498574	2.9872087720381326E-4
25	0.0	0.0	0.0	0.015334338363129082	2.9872087720381326E-4
26	0.0	0.0	0.0	0.018918988889574842	4.978681286730222E-4
27	0.0	0.0	0.0	0.02409681742777427	4.978681286730222E-4
28	0.0	0.0	0.0	0.03395460637550011	4.978681286730222E-4
29	0.0	0.0	0.0	0.04928894473862919	4.978681286730222E-4
30	0.0	0.0	0.0	0.07796614895019527	4.978681286730222E-4
31	0.0	0.0	0.0	0.12924656620351654	4.978681286730222E-4
32	0.0	0.0	0.0	0.19456686468541703	4.978681286730222E-4
33	0.0	0.0	0.0	0.2520208467342838	4.978681286730222E-4
34	0.0	0.0	0.0	0.32819467042125616	4.978681286730222E-4
35	0.0	0.0	0.0	0.4071565556287975	4.978681286730222E-4
36	0.0	0.0	0.0	0.5289350999022187	4.978681286730222E-4
37	0.0	0.0	0.0	0.7111548349965447	4.978681286730222E-4
38	0.0	9.957362573460442E-5	0.0	0.9205581699164179	4.978681286730222E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGCGA	25	0.0023526691	35.1998	44
TCGTATA	50	2.3610319E-6	30.799826	44
CGCAATA	255	0.0	19.843025	36
TAGGACG	365	0.0	19.287561	4
GTCGTAT	80	8.98703E-5	19.249891	43
TAATACT	185	1.8189894E-12	19.026918	4
ACGATTA	70	8.122326E-4	18.857035	40
CTAGCGG	270	0.0	18.740635	29
GTATAGA	130	3.5775884E-8	18.618988	1
AATCCCG	130	3.5857738E-8	18.61528	19
CAATACG	275	0.0	18.399897	38
CGTAAAT	120	3.1608397E-7	18.33323	3
CGCCGGT	300	0.0	18.33323	7
TCTAGCG	305	0.0	18.032684	28
GTCCTAA	260	0.0	17.77267	1
TACGACG	250	0.0	17.599901	5
ACGACGG	250	0.0	17.599901	6
CGATTAA	75	0.0012907131	17.5999	41
CGGTCCA	305	0.0	17.311377	10
CGACGGT	255	0.0	17.254805	7
>>END_MODULE