FastQCFastQC Report
Thu 2 Feb 2017
SRR4062446_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062446_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846898
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT214561.1617317253037254No Hit
GTCCTACAGTGGACATTTCTAAATT195491.0584775120228622No Hit
CTGTAGGACGTGGAATATGGCAAGA187971.017760591001777No Hit
CTTTAGGACGTGAAATATGGCGAGG175870.9522453324439141No Hit
GTCCTACAGTGTGCATTTCTCATTT114890.622070087248998No Hit
CTGAAGGACCTGGAATATGGCGAGA95330.5161627767207502No Hit
CTGTAGGACCTGGAATATGGCGAGA90280.4888196316201544No Hit
GTCCTTCAGTGTGCATTTCTCATTT85630.46364228019089304No Hit
ATTTAGAAATGTCCACTGTAGGACG80470.43570354183068044No Hit
TTTCTAAATTTTCCACCTTTTTCAG65290.3535116720035432No Hit
GAATATGGCAAGAAAACTGAAAATC56550.3061890802848885No Hit
GGAATATGGCGAGAAAACTGAAAAT51620.2794956732856931No Hit
ATTCCAGGTCCTTCAGTGTGCATTT44000.23823730384677444No Hit
ACCTGGAATATGGCGAGAAAACTGA42160.2282746529586366No Hit
GCCATATTCCACGTCCTACAGTGGA41000.22199385131176705No Hit
GAAATATGGCGAGGAAAACTGAAAA40530.21944904374794927No Hit
CTGTAGGACATGGAATATGGCAAGA40520.21939489890616592No Hit
CCATATTCCAGGTCCTTCAGTGTGC39450.21360140083534662No Hit
GACCTGGAATATGGCGAGAAAACTG39350.21305995241751302No Hit
GTATCAACGCAGAGTACTTTTTTTT38930.21078586906261204No Hit
GTCCACTGTAGGACGTGGAATATGG37680.20401776383969228No Hit
TTCCAGGTCCTTCAGTGTGCATTTC37650.20385532931434222No Hit
CCATATTTCACGTCCTAAAGTGTGT37160.2012022320669577No Hit
ACAGTGGACATTTCTAAATTTTCCA36690.19865742450313983No Hit
TTGTAGAACAGTGTATATCAATGAG35900.1943799820022546No Hit
ATACACACTTTAGGACGTGAAATAT35870.19421754747690453No Hit
GATATACACTGTTCTACAAATCCCG35590.1927014919069705No Hit
CCACTGTAGGACGTGGAATATGGCA32940.17835310883438069No Hit
CACTTTAGGACGTGAAATATGGCGA32870.17797409494189717No Hit
CCTAAAGTGTGTATTTCTCATTTTC31840.1723971762382113No Hit
GAAATACACACTTTAGGACGTGAAA31610.1711518448771941No Hit
ATTCCACGTCCTACAGTGGACATTT31150.16866118215515963No Hit
GTGTATTTCTCATTTTCCGTGATTT30480.16503347775567465No Hit
AGTGTGTATTTCTCATTTTCCGTGA30300.16405887060357421No Hit
CTACAGTGGACATTTCTAAATTTTC29780.1612433388308396No Hit
GTGTATATCAATGAGTTACAATGAA28750.15566642012715376No Hit
GTAGGACGTGGAATATGGCAAGAAA28000.15160555699340192No Hit
TATCAACGCAGAGTACTTTTTTTTT27120.14684081091646642No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24220.13113880679929266No Hit
GTCCTACAGTGGACATTTCTAAATA24090.130434923856109No Hit
TCCTAAAGTGTGTATTTCTCATTTT24050.13021834448897557No Hit
GTTCTACAGTGTGGTTTTTATCATT23360.12648235040592387No Hit
GGTCCTTCAGTGTGCATTTCTCATT23330.1263199158805738No Hit
ATATTTCACGTCCTAAAGTGTGTAT22960.12431655673458956No Hit
GTGTATATCAATGAGTTACAATGAG22880.12388339800032269No Hit
TTTTTCAAGTCGTCAAGTGGATGTT22870.12382925315853935No Hit
ACGTGGAATATGGCAAGAAAACTGA22390.12123030075293817No Hit
ACGTCCTACAGTGGACATTTCTAAA21040.11392074711218488No Hit
TTTCTAAATATTCCACCTTTTTCAG20520.11110521533945025No Hit
GTATTTCTCATTTTCCGTGATTTTC19800.10720678673104848No Hit
GATATACACTGTTCTACAATGCCGG19740.10688191768034834No Hit
CCTTCAGTGTGCATTTCTCATTTTT19410.10509513790149752No Hit
GTACATGGAAGCAGTGGTATCAACG19400.10504099305971419No Hit
CACTGAAGGACCTGGAATATGGCGA19160.1037415168569136No Hit
GTGCATTTCTCATTTTTCACGTTTT19040.1030917787555133No Hit
GTGAAATATGGCGAGGAAAACTGAA18920.102442040654113No Hit
CTTGACGACTTGAAAAATGACGAAA18890.10227960612876294No Hit
GTCAAGTGGATGTTTCTCATTTTCC18660.10103427476774571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCC250.00602722619.00287410
AACAGCG508.7061184E-515.2018887
TGTCCGC400.00527430114.25215510
CCTACAC2600.013.8773063
TAGGACC28650.013.5927464
CCAACGA3300.013.53308519
ATGTCCG500.001497717213.3023749
CGCGTAT500.001498329613.3016527
TAAGGCC500.001501701513.2976854
CGTATTC500.00150569513.2929973
ACCTTTT18950.012.88550415
TAGAAAT22600.012.7765374
CCACCTT19100.012.6848213
TCACGAC604.110888E-412.6599993
CACCTTT19550.012.44144314
AAATGTC22800.012.4182097
GTCCTAT3700.012.3138311
TCCAACG5250.012.3079618
AGTGCAC701.0892969E-412.2164668
TTAAGCA550.003072823412.0888044
Produced by FastQC (version 0.11.5)