##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062446_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1846898
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294975683551556	32.0	32.0	32.0	32.0	32.0
2	31.101336402984895	32.0	32.0	32.0	32.0	32.0
3	31.065384769489164	32.0	32.0	32.0	32.0	32.0
4	31.109936769653764	32.0	32.0	32.0	32.0	32.0
5	31.060775419108147	32.0	32.0	32.0	32.0	32.0
6	34.720435021316824	36.0	36.0	36.0	32.0	36.0
7	34.59216589113205	36.0	36.0	36.0	32.0	36.0
8	34.61678609213936	36.0	36.0	36.0	32.0	36.0
9	34.66275560426185	36.0	36.0	36.0	32.0	36.0
10	34.46362387094469	36.0	36.0	36.0	32.0	36.0
11	34.662595876978585	36.0	36.0	36.0	32.0	36.0
12	34.52102714930657	36.0	36.0	36.0	32.0	36.0
13	34.57357092811839	36.0	36.0	36.0	32.0	36.0
14	34.53700962370418	36.0	36.0	36.0	32.0	36.0
15	34.50855217775968	36.0	36.0	36.0	32.0	36.0
16	34.55437874749986	36.0	36.0	36.0	32.0	36.0
17	34.53279065763242	36.0	36.0	36.0	32.0	36.0
18	34.565472484132854	36.0	36.0	36.0	32.0	36.0
19	34.43058035690114	36.0	36.0	36.0	32.0	36.0
20	34.40416146424978	36.0	36.0	36.0	32.0	36.0
21	34.3527330691787	36.0	36.0	36.0	32.0	36.0
22	34.309886631530276	36.0	36.0	36.0	32.0	36.0
23	34.352866806937904	36.0	36.0	36.0	32.0	36.0
24	34.28680576837486	36.0	36.0	36.0	32.0	36.0
25	33.92517561879433	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	10.0
4	64.0
5	170.0
6	309.0
7	59.0
8	258.0
9	349.0
10	224.0
11	64.0
12	168.0
13	109.0
14	320.0
15	357.0
16	548.0
17	714.0
18	876.0
19	1237.0
20	1767.0
21	2564.0
22	3633.0
23	5371.0
24	7452.0
25	10764.0
26	15542.0
27	20442.0
28	28609.0
29	39590.0
30	52222.0
31	73469.0
32	106388.0
33	159032.0
34	387579.0
35	926636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.065110068180374	21.313692012699278	12.881169252062714	25.74002866705763
2	10.400180199399731	20.772258389045522	50.91569776098998	17.911863650564776
3	18.649742284369257	27.35521872121715	30.64761103377025	23.347427960643337
4	10.291838465362458	18.09991996265646	40.95031078099768	30.657930790983396
5	9.253127787710703	41.60348984142648	37.775561902275555	11.367820468587258
6	27.90591602732966	40.10090717872131	20.171739381263702	11.821437412685334
7	25.348531402908314	32.469456940639	24.146205747171898	18.03580590928079
8	20.40214670447913	46.96314368753885	20.442845862911575	12.191863745070442
9	26.60733138656382	15.278726231743617	22.43337517370257	35.680567207989995
10	15.288706938984381	30.021607755807423	36.97566227283668	17.71402303237152
11	31.809806322767198	19.806610081928067	32.02057607145355	16.363007523851188
12	26.809728803634748	23.322074147970635	35.83917979635874	14.02901725203587
13	30.91126822715351	24.790826167519047	26.72979739114124	17.5681082141862
14	17.044832338179535	27.862155929233666	28.199897430670834	26.893114301915965
15	17.67979640014202	44.210928585561	22.24360972357362	15.86566529072336
16	15.448465994619504	28.53094436232221	38.79915069890628	17.221438944152005
17	16.572030172362386	34.24478559602559	32.95437172896918	16.228812502642846
18	16.61901059710507	27.06588248306014	42.13815814441686	14.176948775417925
19	23.879256969301352	25.662665103776416	28.46122900860204	21.99684891832019
20	23.539906485057937	30.213241173680288	31.754584265094532	14.492268076167242
21	18.787863015986094	27.73326653936778	27.5880221938131	25.890848250833027
22	22.079490438675645	35.82465338030124	27.836132175331436	14.259724005691677
23	15.889835907707505	35.08261005591923	32.60010616684027	16.42744786953299
24	23.339823364872608	27.912015341848996	33.76670695149067	14.981454341787725
25	18.741396594040683	32.05956389711316	33.65768975745376	15.541349751392403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	403.0
1	403.0
2	820.0
3	1237.0
4	1237.0
5	1237.0
6	2797.0
7	4357.0
8	4357.0
9	4357.0
10	4881.0
11	5405.0
12	5405.0
13	5405.0
14	7024.0
15	8643.0
16	8643.0
17	8643.0
18	15929.5
19	23216.0
20	23216.0
21	23216.0
22	45209.0
23	67202.0
24	67202.0
25	67202.0
26	112250.0
27	157298.0
28	157298.0
29	157298.0
30	217203.5
31	277109.0
32	277109.0
33	277109.0
34	286540.0
35	295971.0
36	295971.0
37	295971.0
38	286596.0
39	277221.0
40	277221.0
41	277221.0
42	266006.5
43	254792.0
44	254792.0
45	254792.0
46	243145.5
47	231499.0
48	231499.0
49	231499.0
50	187884.5
51	144270.0
52	144270.0
53	144270.0
54	97698.0
55	51126.0
56	51126.0
57	51126.0
58	37419.5
59	23713.0
60	23713.0
61	23713.0
62	17654.5
63	11596.0
64	11596.0
65	11596.0
66	8940.0
67	6284.0
68	6284.0
69	6284.0
70	4480.5
71	2677.0
72	2677.0
73	2677.0
74	1946.0
75	1215.0
76	1215.0
77	1215.0
78	897.5
79	580.0
80	580.0
81	580.0
82	406.5
83	233.0
84	233.0
85	233.0
86	163.5
87	94.0
88	94.0
89	94.0
90	73.0
91	52.0
92	52.0
93	52.0
94	52.0
95	52.0
96	52.0
97	52.0
98	352.5
99	653.0
100	653.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009691926679221051
2	0.0029779662980846805
3	0.005143759969418993
4	0.014077658863673036
5	0.03384052611459864
6	0.04575239130693736
7	0.06632743118461334
8	0.08939313378432377
9	0.09691926679221051
10	0.11857720350555365
11	0.12079714201867131
12	0.1341709179391607
13	0.12236734243038869
14	0.12409997736745612
15	0.1138666022704015
16	0.11300028480186779
17	0.12431655673458956
18	0.13168025521712623
19	0.13276315205279338
20	0.12307122537357233
21	0.13103051711572591
22	0.1517138466769686
23	0.14245507873201443
24	0.13704059455367865
25	0.13130124132464271
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1846898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.13184191532194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.38544506807169	33.06401418832721
2	11.925305336498937	9.81019547785639
3	3.0626323982010026	3.779151349426409
4	1.2616111742460878	2.075695655107751
5	0.6814458000753567	1.4014560461279828
6	0.43529287270670947	1.0742638576223238
7	0.30202674136328633	0.8696041325968163
8	0.22998000047395775	0.7567600818544397
9	0.1774780568182769	0.6569999438839103
>10	1.2608177941492558	10.181919638099574
>50	0.13528950552779034	3.8696049654273077
>100	0.11356064076334452	9.53030771592272
>500	0.014491435391301342	4.14971156194052
>1k	0.013042291852171208	11.1185132899791
>5k	9.221822521737217E-4	2.8457502787692808
>10k+	6.587016086955156E-4	4.816051817058402
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	21456	1.1617317253037254	No Hit
GTCCTACAGTGGACATTTCTAAATT	19549	1.0584775120228622	No Hit
CTGTAGGACGTGGAATATGGCAAGA	18797	1.017760591001777	No Hit
CTTTAGGACGTGAAATATGGCGAGG	17587	0.9522453324439141	No Hit
GTCCTACAGTGTGCATTTCTCATTT	11489	0.622070087248998	No Hit
CTGAAGGACCTGGAATATGGCGAGA	9533	0.5161627767207502	No Hit
CTGTAGGACCTGGAATATGGCGAGA	9028	0.4888196316201544	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	8563	0.46364228019089304	No Hit
ATTTAGAAATGTCCACTGTAGGACG	8047	0.43570354183068044	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	6529	0.3535116720035432	No Hit
GAATATGGCAAGAAAACTGAAAATC	5655	0.3061890802848885	No Hit
GGAATATGGCGAGAAAACTGAAAAT	5162	0.2794956732856931	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	4400	0.23823730384677444	No Hit
ACCTGGAATATGGCGAGAAAACTGA	4216	0.2282746529586366	No Hit
GCCATATTCCACGTCCTACAGTGGA	4100	0.22199385131176705	No Hit
GAAATATGGCGAGGAAAACTGAAAA	4053	0.21944904374794927	No Hit
CTGTAGGACATGGAATATGGCAAGA	4052	0.21939489890616592	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	3945	0.21360140083534662	No Hit
GACCTGGAATATGGCGAGAAAACTG	3935	0.21305995241751302	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3893	0.21078586906261204	No Hit
GTCCACTGTAGGACGTGGAATATGG	3768	0.20401776383969228	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	3765	0.20385532931434222	No Hit
CCATATTTCACGTCCTAAAGTGTGT	3716	0.2012022320669577	No Hit
ACAGTGGACATTTCTAAATTTTCCA	3669	0.19865742450313983	No Hit
TTGTAGAACAGTGTATATCAATGAG	3590	0.1943799820022546	No Hit
ATACACACTTTAGGACGTGAAATAT	3587	0.19421754747690453	No Hit
GATATACACTGTTCTACAAATCCCG	3559	0.1927014919069705	No Hit
CCACTGTAGGACGTGGAATATGGCA	3294	0.17835310883438069	No Hit
CACTTTAGGACGTGAAATATGGCGA	3287	0.17797409494189717	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	3184	0.1723971762382113	No Hit
GAAATACACACTTTAGGACGTGAAA	3161	0.1711518448771941	No Hit
ATTCCACGTCCTACAGTGGACATTT	3115	0.16866118215515963	No Hit
GTGTATTTCTCATTTTCCGTGATTT	3048	0.16503347775567465	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	3030	0.16405887060357421	No Hit
CTACAGTGGACATTTCTAAATTTTC	2978	0.1612433388308396	No Hit
GTGTATATCAATGAGTTACAATGAA	2875	0.15566642012715376	No Hit
GTAGGACGTGGAATATGGCAAGAAA	2800	0.15160555699340192	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2712	0.14684081091646642	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2422	0.13113880679929266	No Hit
GTCCTACAGTGGACATTTCTAAATA	2409	0.130434923856109	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	2405	0.13021834448897557	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2336	0.12648235040592387	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	2333	0.1263199158805738	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	2296	0.12431655673458956	No Hit
GTGTATATCAATGAGTTACAATGAG	2288	0.12388339800032269	No Hit
TTTTTCAAGTCGTCAAGTGGATGTT	2287	0.12382925315853935	No Hit
ACGTGGAATATGGCAAGAAAACTGA	2239	0.12123030075293817	No Hit
ACGTCCTACAGTGGACATTTCTAAA	2104	0.11392074711218488	No Hit
TTTCTAAATATTCCACCTTTTTCAG	2052	0.11110521533945025	No Hit
GTATTTCTCATTTTCCGTGATTTTC	1980	0.10720678673104848	No Hit
GATATACACTGTTCTACAATGCCGG	1974	0.10688191768034834	No Hit
CCTTCAGTGTGCATTTCTCATTTTT	1941	0.10509513790149752	No Hit
GTACATGGAAGCAGTGGTATCAACG	1940	0.10504099305971419	No Hit
CACTGAAGGACCTGGAATATGGCGA	1916	0.1037415168569136	No Hit
GTGCATTTCTCATTTTTCACGTTTT	1904	0.1030917787555133	No Hit
GTGAAATATGGCGAGGAAAACTGAA	1892	0.102442040654113	No Hit
CTTGACGACTTGAAAAATGACGAAA	1889	0.10227960612876294	No Hit
GTCAAGTGGATGTTTCTCATTTTCC	1866	0.10103427476774571	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.4144841783357824E-5	0.0
8	0.0	0.0	0.0	5.4144841783357824E-5	0.0
9	0.0	0.0	0.0	1.0828968356671565E-4	0.0
10	0.0	0.0	0.0	1.0828968356671565E-4	0.0
11	0.0	0.0	0.0	1.6243452535007349E-4	0.0
12	0.0	0.0	0.0	1.6243452535007349E-4	0.0
13	0.0	0.0	0.0	1.6243452535007349E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTCC	25	0.006027226	19.002874	10
AACAGCG	50	8.7061184E-5	15.201888	7
TGTCCGC	40	0.005274301	14.252155	10
CCTACAC	260	0.0	13.877306	3
TAGGACC	2865	0.0	13.592746	4
CCAACGA	330	0.0	13.533085	19
ATGTCCG	50	0.0014977172	13.302374	9
CGCGTAT	50	0.0014983296	13.301652	7
TAAGGCC	50	0.0015017015	13.297685	4
CGTATTC	50	0.001505695	13.292997	3
ACCTTTT	1895	0.0	12.885504	15
TAGAAAT	2260	0.0	12.776537	4
CCACCTT	1910	0.0	12.68482	13
TCACGAC	60	4.110888E-4	12.659999	3
CACCTTT	1955	0.0	12.441443	14
AAATGTC	2280	0.0	12.418209	7
GTCCTAT	370	0.0	12.313831	1
TCCAACG	525	0.0	12.30796	18
AGTGCAC	70	1.0892969E-4	12.216466	8
TTAAGCA	55	0.0030728234	12.088804	4
>>END_MODULE