FastQCFastQC Report
Thu 2 Feb 2017
SRR4062446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846898
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA106650.5774547376195112No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA86660.4692191988945789No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA78900.42720280167069324No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC77200.41799817856752236No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA53260.2883754273381638No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA41950.2271376112811861No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA33000.1786779778850808No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT30700.16622466427490853No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA27060.14651594186576627No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA26760.14489159661226556No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT24430.13227584847674317No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG23120.1251828742031233No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC22610.12242148727217203No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA20860.11294613996008442No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA20820.11272956059295099No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACA20350.11018475302913316No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC19900.10774823514888207No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG19800.10720678673104848No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT19760.10699020736391505No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG19010.10292934423016323No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG18520.10027624698277868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGA353.2186657E-431.42832236
ATCGCGG407.033874E-427.4997835
TAGGACC36200.026.983944
GCGGGGT905.14774E-1026.88867444
CTGCGCA450.001398558324.4442489
CACTCGG551.594149E-423.9998114
ACTGTTC15650.022.7729828
ATGGGCG1407.2759576E-1222.0004225
TTGGACG1004.6264176E-822.000424
AGGCGGG1205.984475E-1021.99982542
CGAGGAC904.0845407E-721.99982521
AGGACCT76000.021.2477745
GATATAC17450.021.1849351
GCGATAA1259.968062E-1021.11983120
TGTAGGA123700.021.1111552
CTGTAGG120450.020.990681
GGACCTG75200.020.9179526
GTCTTAC3300.020.6709791
ATAGGAC6300.020.6035693
GCGACAA755.474976E-520.5331720
Produced by FastQC (version 0.11.5)