##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062446_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1846898
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.373467294891217	32.0	32.0	32.0	32.0	32.0
2	31.54895614159526	32.0	32.0	32.0	32.0	32.0
3	31.59774443418099	32.0	32.0	32.0	32.0	32.0
4	31.669486890992356	32.0	32.0	32.0	32.0	32.0
5	31.651333749887648	32.0	32.0	32.0	32.0	32.0
6	35.27381912807313	36.0	36.0	36.0	36.0	36.0
7	35.2944943359081	36.0	36.0	36.0	36.0	36.0
8	35.229450137473755	36.0	36.0	36.0	36.0	36.0
9	35.34528436329456	36.0	36.0	36.0	36.0	36.0
10	35.263378919680456	36.0	36.0	36.0	36.0	36.0
11	35.33893479769863	36.0	36.0	36.0	36.0	36.0
12	35.30474666169978	36.0	36.0	36.0	36.0	36.0
13	35.31286676362203	36.0	36.0	36.0	36.0	36.0
14	35.29574941334064	36.0	36.0	36.0	36.0	36.0
15	35.247440302604694	36.0	36.0	36.0	36.0	36.0
16	35.297584382028674	36.0	36.0	36.0	36.0	36.0
17	35.307456611031036	36.0	36.0	36.0	36.0	36.0
18	35.309481086665315	36.0	36.0	36.0	36.0	36.0
19	35.25948265686573	36.0	36.0	36.0	36.0	36.0
20	35.23372974576831	36.0	36.0	36.0	36.0	36.0
21	35.19692316522082	36.0	36.0	36.0	36.0	36.0
22	35.17715163479521	36.0	36.0	36.0	36.0	36.0
23	35.17369448664734	36.0	36.0	36.0	36.0	36.0
24	35.163328456687914	36.0	36.0	36.0	36.0	36.0
25	35.11372528423335	36.0	36.0	36.0	36.0	36.0
26	35.09269705202995	36.0	36.0	36.0	36.0	36.0
27	35.02506635450361	36.0	36.0	36.0	36.0	36.0
28	34.96953161463167	36.0	36.0	36.0	36.0	36.0
29	34.94217060173329	36.0	36.0	36.0	36.0	36.0
30	34.88108926426906	36.0	36.0	36.0	36.0	36.0
31	34.87392319445903	36.0	36.0	36.0	36.0	36.0
32	34.84341799059829	36.0	36.0	36.0	36.0	36.0
33	34.88849465427977	36.0	36.0	36.0	36.0	36.0
34	34.84471096942008	36.0	36.0	36.0	36.0	36.0
35	34.83147472139772	36.0	36.0	36.0	36.0	36.0
36	34.74760490292371	36.0	36.0	36.0	36.0	36.0
37	34.65596963124114	36.0	36.0	36.0	32.0	36.0
38	34.621917940243584	36.0	36.0	36.0	32.0	36.0
39	34.40344566944141	36.0	36.0	36.0	32.0	36.0
40	34.515842239257395	36.0	36.0	36.0	32.0	36.0
41	34.47207588074707	36.0	36.0	36.0	32.0	36.0
42	34.48806539397411	36.0	36.0	36.0	32.0	36.0
43	34.46131188620054	36.0	36.0	36.0	32.0	36.0
44	34.35601587093603	36.0	36.0	36.0	32.0	36.0
45	34.344024412826265	36.0	36.0	36.0	32.0	36.0
46	34.17116267384555	36.0	36.0	36.0	32.0	36.0
47	34.2044157284268	36.0	36.0	36.0	32.0	36.0
48	34.14283842421184	36.0	36.0	36.0	32.0	36.0
49	34.070483047791484	36.0	36.0	36.0	32.0	36.0
50	33.70730381428753	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	20.0
21	33.0
22	136.0
23	347.0
24	979.0
25	2402.0
26	5440.0
27	10749.0
28	18432.0
29	28506.0
30	41144.0
31	58234.0
32	85321.0
33	136847.0
34	288080.0
35	1170224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.59688550583994	22.032154368696496	13.199017433195856	26.171942692267702
2	10.182796182175654	21.27775933758246	50.30471445101854	18.234730029223346
3	18.49869943667524	27.683318786371913	30.962503492353637	22.855478284599208
4	10.28154326295203	18.648193830500105	41.2749635604031	29.795299346144766
5	9.131473421921514	41.96403916188117	37.450091992086186	11.454395424111132
6	28.317309983063403	40.03502107328652	19.863954933921278	11.783714009728797
7	25.40336575347732	32.58248835749909	24.23502772488125	17.779118164142343
8	20.490736359019284	47.03037200754996	20.284173787615774	12.194717845814983
9	26.303130979620963	15.24713330135178	22.430421171066296	36.01931454796096
10	15.374211245017321	30.361720030017903	36.503369433504176	17.7606992914606
11	31.62156220863307	19.811705898214193	32.30616958814185	16.26056230501089
12	26.890169354236132	23.35445704094108	35.67841862409294	14.07695498072985
13	30.86483389986886	24.72908628413697	26.793304232285703	17.61277558370847
14	17.097045965721982	27.960179717558848	28.153043643991165	26.789730672728002
15	17.744130969874895	44.20092501047703	22.109017390240286	15.945926629407797
16	15.598804048734689	28.710897948885105	38.259070073171344	17.431227929208866
17	16.741530934572456	34.55372197056903	32.437200105257574	16.26754698960094
18	16.798058149394283	27.270807591973135	41.700083058187296	14.231051200445288
19	24.149303318320772	25.650090042871888	28.670776621123633	21.529830017683704
20	23.84186890667487	30.354302186693584	31.493617947498997	14.310210959132558
21	18.97635927918055	27.523772292784983	27.847991605383733	25.65187682265074
22	22.483158246963285	35.30162467012255	28.027265176528427	14.187951906385734
23	16.047881366485857	35.03117118541468	32.39447982509051	16.52646762300896
24	23.60742174175293	27.99661919607905	33.323009716833305	15.072949345334719
25	19.12282107620453	32.11016526088609	33.058187295670905	15.708826367238471
26	14.020915069484074	34.502067791507706	34.61398517947391	16.86303195953431
27	16.4658254002116	33.88048500783476	31.99532405146359	17.658365540490053
28	15.222876412232836	34.48024742026901	29.228955795068273	21.067920372429878
29	19.416015394461418	29.941068754202995	28.10371769312653	22.53919815820906
30	15.681429077296094	30.40070431610192	32.45820830386952	21.459658302732475
31	22.155527809332188	27.511968717276215	31.09716941596125	19.23533405743035
32	17.574711759934765	33.11173654419464	31.432380131442017	17.881171564428573
33	15.198348798904975	33.990615616022104	35.502556177980594	15.308479407092324
34	17.82150394878331	32.82254894422973	34.32512244855969	15.030824658427267
35	15.953885921149949	34.45626125535898	33.150829119962225	16.439023703528836
36	14.76481105074563	32.190028902516545	35.70549104498462	17.33966900175321
37	16.05259196772101	31.946918562909264	29.036470882528437	22.964018586841288
38	18.074576939278725	35.929650689967715	28.796717523111724	17.19905484764183
39	21.873758052691596	28.646249007795777	30.563842724395172	18.916150215117455
40	23.547862415791236	28.452681198420272	32.62464954751156	15.374806838276939
41	22.58240574195218	26.092074386349434	34.64658037422749	16.678939497470893
42	18.815007650666143	33.529951302129305	33.008428186071995	14.646612861132557
43	15.055027402704427	34.62221519542498	33.90842374619497	16.414333655675627
44	14.52586986395567	33.715884688813354	32.289817845923274	19.468427601307706
45	15.240419340970643	34.432329235290744	31.264422832230043	19.062828591508573
46	17.10784857828951	28.332200801886408	32.863752406065316	21.69619821375877
47	22.490467800604037	28.57066280866621	30.9164339340884	18.022435456641354
48	15.401391955592567	31.345910819113996	30.81724058394129	22.435456641352147
49	20.405566522894063	28.670614186598286	30.806357470742835	20.117461819764817
50	15.87797485296968	35.989968043714384	28.805218263271716	19.326838840044225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80.0
1	67.5
2	55.0
3	198.5
4	342.0
5	372.0
6	402.0
7	376.0
8	350.0
9	474.5
10	599.0
11	891.0
12	1183.0
13	1970.5
14	2758.0
15	3744.5
16	4731.0
17	5580.5
18	6430.0
19	7237.0
20	8044.0
21	9436.5
22	10829.0
23	13158.5
24	15488.0
25	20374.0
26	25260.0
27	38052.5
28	50845.0
29	69917.5
30	88990.0
31	116436.0
32	143882.0
33	186054.0
34	228226.0
35	268373.5
36	308521.0
37	296406.0
38	284291.0
39	244129.0
40	203967.0
41	167975.5
42	131984.0
43	109098.5
44	86213.0
45	74953.5
46	63694.0
47	56703.0
48	49712.0
49	43727.5
50	37743.0
51	32340.0
52	26937.0
53	23105.0
54	19273.0
55	16622.0
56	13971.0
57	12015.0
58	10059.0
59	8460.0
60	6861.0
61	5772.5
62	4684.0
63	3994.5
64	3305.0
65	2818.5
66	2332.0
67	2047.5
68	1763.0
69	1442.0
70	1121.0
71	946.5
72	772.0
73	646.0
74	520.0
75	422.0
76	324.0
77	248.5
78	173.0
79	126.5
80	80.0
81	64.0
82	48.0
83	35.5
84	23.0
85	14.5
86	6.0
87	7.0
88	8.0
89	7.0
90	6.0
91	5.0
92	4.0
93	4.0
94	4.0
95	2.5
96	1.0
97	2.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016568321585707493
2	0.00471060123515213
3	3.2486905070014697E-4
4	3.2486905070014697E-4
5	0.0
6	5.414484178335782E-4
7	5.4144841783357824E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.6243452535007349E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1846898.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.33203527981093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.81435792785517	46.90583655114872
2	11.46548500166881	13.146791812316385
3	2.9441153970969034	5.063763834425828
4	1.2012283304871127	2.7547546009038215
5	0.5983917322975165	1.7153507953614204
6	0.37887366076971984	1.303295885150442
7	0.26238416870110814	1.0530112891785057
8	0.194750532850036	0.8932355536096193
9	0.13996824608974345	0.7222197980583387
>10	0.8601425250190479	9.176701147529796
>50	0.07843809680913066	3.134928959221773
>100	0.05218710193035086	5.849232530997092
>500	0.005407890884801212	2.1216351711982164
>1k	0.0037950111472289205	3.9689617622566407
>5k	3.79501114722892E-4	1.6101690639100015
>10k+	9.4875278680723E-5	0.5801112447334695
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	10665	0.5774547376195112	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	8666	0.4692191988945789	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	7890	0.42720280167069324	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	7720	0.41799817856752236	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	5326	0.2883754273381638	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	4195	0.2271376112811861	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	3300	0.1786779778850808	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	3070	0.16622466427490853	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2706	0.14651594186576627	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	2676	0.14489159661226556	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	2443	0.13227584847674317	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	2312	0.1251828742031233	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC	2261	0.12242148727217203	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2086	0.11294613996008442	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	2082	0.11272956059295099	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACA	2035	0.11018475302913316	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1990	0.10774823514888207	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	1980	0.10720678673104848	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT	1976	0.10699020736391505	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1901	0.10292934423016323	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG	1852	0.10027624698277868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.4144841783357824E-5	0.0
10	0.0	0.0	0.0	5.4144841783357824E-5	0.0
11	0.0	0.0	0.0	1.0828968356671565E-4	0.0
12	0.0	0.0	0.0	1.0828968356671565E-4	0.0
13	0.0	0.0	0.0	1.0828968356671565E-4	0.0
14	0.0	0.0	0.0	1.0828968356671565E-4	0.0
15	0.0	0.0	0.0	1.0828968356671565E-4	0.0
16	0.0	0.0	0.0	1.6243452535007349E-4	0.0
17	0.0	0.0	0.0	2.165793671334313E-4	0.0
18	0.0	0.0	0.0	2.707242089167891E-4	0.0
19	0.0	0.0	0.0	3.2486905070014697E-4	0.0
20	0.0	0.0	0.0	7.038829431836517E-4	0.0
21	0.0	0.0	0.0	0.0010828968356671564	0.0
22	5.4144841783357824E-5	0.0	0.0	0.002165793671334313	0.0
23	5.4144841783357824E-5	0.0	0.0	0.0032486905070014693	0.0
24	5.4144841783357824E-5	0.0	0.0	0.005089615127635635	0.0
25	5.4144841783357824E-5	0.0	0.0	0.0062808016468695075	0.0
26	5.4144841783357824E-5	0.0	0.0	0.008338305634637106	0.0
27	5.4144841783357824E-5	0.0	0.0	0.010828968356671565	0.0
28	5.4144841783357824E-5	0.0	0.0	0.016568321585707493	0.0
29	5.4144841783357824E-5	0.0	0.0	0.026206103423145187	0.0
30	5.4144841783357824E-5	0.0	0.0	0.04537337741445386	0.0
31	5.4144841783357824E-5	0.0	0.0	0.09919335014711153	0.0
32	5.4144841783357824E-5	5.4144841783357824E-5	0.0	0.17607902547947965	0.0
33	5.4144841783357824E-5	5.4144841783357824E-5	0.0	0.25199009365974734	0.0
34	5.4144841783357824E-5	5.4144841783357824E-5	0.0	0.33374880475261764	0.0
35	5.4144841783357824E-5	5.4144841783357824E-5	0.0	0.42297950401159135	0.0
36	5.4144841783357824E-5	5.4144841783357824E-5	0.0	0.549732578626432	0.0
37	5.4144841783357824E-5	5.4144841783357824E-5	0.0	0.7600311441129938	0.0
38	5.4144841783357824E-5	5.4144841783357824E-5	0.0	1.0087184024239564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGGA	35	3.2186657E-4	31.428322	36
ATCGCGG	40	7.033874E-4	27.49978	35
TAGGACC	3620	0.0	26.98394	4
GCGGGGT	90	5.14774E-10	26.888674	44
CTGCGCA	45	0.0013985583	24.444248	9
CACTCGG	55	1.594149E-4	23.99981	14
ACTGTTC	1565	0.0	22.772982	8
ATGGGCG	140	7.2759576E-12	22.000422	5
TTGGACG	100	4.6264176E-8	22.00042	4
AGGCGGG	120	5.984475E-10	21.999825	42
CGAGGAC	90	4.0845407E-7	21.999825	21
AGGACCT	7600	0.0	21.247774	5
GATATAC	1745	0.0	21.184935	1
GCGATAA	125	9.968062E-10	21.119831	20
TGTAGGA	12370	0.0	21.111155	2
CTGTAGG	12045	0.0	20.99068	1
GGACCTG	7520	0.0	20.917952	6
GTCTTAC	330	0.0	20.670979	1
ATAGGAC	630	0.0	20.603569	3
GCGACAA	75	5.474976E-5	20.53317	20
>>END_MODULE