FastQCFastQC Report
Thu 2 Feb 2017
SRR4062445_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062445_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726730
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG70660.4092127894922773No Hit
TCCATGTACTCTGCGTTGATACCAC59480.3444661296207282No Hit
GTATCAACGCAGAGTACTTTTTTTT33100.19169181053204612No Hit
GAGTACATGGAAGCAGTGGTATCAA31370.1816728730027277No Hit
CATGTACTCTGCGTTGATACCACTG27890.16151917207669989No Hit
GTCTTGCGCCGGTCCAAGAATTTCA24610.14252372982458172No Hit
TATCAACGCAGAGTACTTTTTTTTT24410.14136547115067208No Hit
GCGCAAGACGGACCAGAGCGAAAGC24370.14113381941589015No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24090.13951225727241665No Hit
GAATAGGACCGCGGTTCTATTTTGT22520.13041992668222593No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21410.12399159104202742No Hit
GAATAACGCCGCCGCATCGCCAGTC21380.12381785224094097No Hit
GTCCTATTCCATTATTCCTAGCTGC20580.1191848175453024No Hit
GTATCTGATCGTCTTCGAACCTCCG20560.11906899167791142No Hit
GAACTACGACGGTATCTGATCGTCT20290.1175053424681334No Hit
GATTAAGAGGGACGGCCGGGGGCAT19450.1126406560377129No Hit
GCTTCCATGTACTCTGCGTTGATAC18900.10945544468446138No Hit
GGGTAGGCACACGCTGAGCCAGTCA18720.10841301187794271No Hit
CTATTGGAGCTGGAATTACCGCGGC18620.10783388254098787No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG17700.10250589264100352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2150.014.58388816
TCCGATA2200.013.8197448
GTTCTAG1051.3860699E-913.5615551
GATAACG2200.013.38748811
CGATAAC2200.013.38748810
AACCGCG1153.765308E-1013.2188847
CGCGGTC1153.765308E-1013.21850110
CCGATAA2300.013.2185019
CGCATCG3250.013.15571413
AATTCCG2400.013.0613235
ATCGCCA3250.012.86373816
GCATCGC3250.012.86336514
ATAACGA2450.012.79773512
CGCCAGT3400.012.57604518
TAACGAA2500.012.5417813
ACGAACG2350.012.53405915
CGTCTTA1301.9645086E-1012.425215
TTATACA1301.9826984E-1012.4205174
AGAACCG1850.012.3232155
AAGTGCG550.003066083612.0922777
Produced by FastQC (version 0.11.5)