##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062445_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1726730
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13090407880792	32.0	32.0	32.0	32.0	32.0
2	30.733637569278347	32.0	32.0	32.0	32.0	32.0
3	30.72494599618933	32.0	32.0	32.0	32.0	32.0
4	30.801449560730397	32.0	32.0	32.0	32.0	32.0
5	30.561421299218754	32.0	32.0	32.0	32.0	32.0
6	34.223154749150126	36.0	36.0	36.0	32.0	36.0
7	34.090614050835974	36.0	36.0	36.0	32.0	36.0
8	34.08002525003909	36.0	36.0	36.0	32.0	36.0
9	34.271671309353515	36.0	36.0	36.0	32.0	36.0
10	33.87084662917769	36.0	36.0	36.0	32.0	36.0
11	34.26078020304274	36.0	36.0	36.0	32.0	36.0
12	33.99499979730473	36.0	36.0	36.0	32.0	36.0
13	34.143695887602576	36.0	36.0	36.0	32.0	36.0
14	34.030849640650246	36.0	36.0	36.0	32.0	36.0
15	33.950771110712154	36.0	36.0	36.0	32.0	36.0
16	33.95036340365894	36.0	36.0	36.0	32.0	36.0
17	33.864568867165104	36.0	36.0	36.0	32.0	36.0
18	33.8879158872551	36.0	36.0	36.0	32.0	36.0
19	33.873287080203625	36.0	36.0	36.0	32.0	36.0
20	33.86941965449144	36.0	36.0	36.0	32.0	36.0
21	33.82761057026866	36.0	36.0	36.0	32.0	36.0
22	33.78728231976047	36.0	36.0	36.0	32.0	36.0
23	33.735311832191485	36.0	36.0	36.0	27.0	36.0
24	33.706525629368805	36.0	36.0	36.0	27.0	36.0
25	33.23292639845257	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	61.0
5	158.0
6	284.0
7	109.0
8	238.0
9	298.0
10	193.0
11	75.0
12	121.0
13	98.0
14	299.0
15	340.0
16	532.0
17	733.0
18	1014.0
19	1509.0
20	2406.0
21	3618.0
22	5605.0
23	8373.0
24	11887.0
25	16790.0
26	23078.0
27	29573.0
28	39783.0
29	52834.0
30	67299.0
31	90218.0
32	128838.0
33	181755.0
34	388684.0
35	669918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.402696652640905	16.832707338474503	10.850783483002575	25.913812525882012
2	17.08787119753283	19.686333560166215	36.272257373275224	26.95353786902573
3	19.478239717835933	23.275524062421272	28.038143114866603	29.20809310487619
4	12.7529571764019	14.644669668539073	34.88541412053904	37.71695903451999
5	14.955113513914348	36.161892601492816	33.14045507527813	15.742538809314699
6	35.64024807121386	34.270746183564356	16.278688448604715	13.810317296617065
7	30.773376510860928	30.05918558822524	20.43551506181368	18.731922839100157
8	28.856426759655555	32.21071582588696	19.355863354555698	19.576994059901786
9	27.237013721023253	14.072309270820652	18.308609985565973	40.38206702259012
10	16.06807287840476	26.26358149710004	31.17335901537734	26.49498660911786
11	38.2343595987708	20.657853539746043	21.528265785353977	19.579521076129183
12	24.70921095818599	23.405081979951824	28.337622633587827	23.548084428274354
13	29.847175586594222	18.808007843869426	25.13650524538474	26.208311324151616
14	23.842408576413472	19.550548898115952	24.27391967121197	32.333122854258605
15	25.725919273567627	26.8455768828273	22.254586439225584	25.173917404379488
16	26.580211118049046	25.21310956466686	23.581806069861326	24.62487324742277
17	24.3076188024316	25.686176961060585	25.265622499460754	24.740581737047062
18	25.67778127640278	23.809051619409136	26.46149260620695	24.05167449798114
19	25.713561687763807	25.327590935950077	24.689544634536844	24.269302741749264
20	25.54516104342502	23.879700433948656	25.257562790352473	25.317575732273855
21	26.87398376812401	24.185622797922655	23.69754155967963	25.242851874273708
22	25.718423554235493	24.374462418445653	24.442147669846772	25.464966357472086
23	24.17585412799292	24.02142010866634	25.098978694136555	26.703747069204187
24	24.796193524816896	25.312596046229768	24.65771298342061	25.233497445532727
25	25.305335942021255	24.223801300555408	24.58118149439778	25.88968126302556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	171.0
1	171.0
2	479.0
3	787.0
4	787.0
5	787.0
6	2218.0
7	3649.0
8	3649.0
9	3649.0
10	3783.5
11	3918.0
12	3918.0
13	3918.0
14	4091.0
15	4264.0
16	4264.0
17	4264.0
18	6798.0
19	9332.0
20	9332.0
21	9332.0
22	15558.0
23	21784.0
24	21784.0
25	21784.0
26	33404.5
27	45025.0
28	45025.0
29	45025.0
30	55515.5
31	66006.0
32	66006.0
33	66006.0
34	82573.5
35	99141.0
36	99141.0
37	99141.0
38	112962.0
39	126783.0
40	126783.0
41	126783.0
42	148875.5
43	170968.0
44	170968.0
45	170968.0
46	206944.5
47	242921.0
48	242921.0
49	242921.0
50	237898.0
51	232875.0
52	232875.0
53	232875.0
54	215282.5
55	197690.0
56	197690.0
57	197690.0
58	184617.0
59	171544.0
60	171544.0
61	171544.0
62	153602.0
63	135660.0
64	135660.0
65	135660.0
66	113115.0
67	90570.0
68	90570.0
69	90570.0
70	68586.0
71	46602.0
72	46602.0
73	46602.0
74	36362.0
75	26122.0
76	26122.0
77	26122.0
78	22118.0
79	18114.0
80	18114.0
81	18114.0
82	12679.0
83	7244.0
84	7244.0
85	7244.0
86	5280.5
87	3317.0
88	3317.0
89	3317.0
90	2276.5
91	1236.0
92	1236.0
93	1236.0
94	735.0
95	234.0
96	234.0
97	234.0
98	503.5
99	773.0
100	773.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00839737538584492
2	0.0031852113532515216
3	0.004343470027161166
4	0.014594059291261516
5	0.03463193434989836
6	0.04482461068030323
7	0.0660786573465452
8	0.0872168781453962
9	0.09497721126059083
10	0.11866360114204307
11	0.1175632554018289
12	0.13227314056048137
13	0.11993768568334368
14	0.12121177022464426
15	0.11101909389423939
16	0.11078744215945747
17	0.12173298662790362
18	0.1298987102789666
19	0.13013036201374853
20	0.12173298662790362
21	0.12868253867136148
22	0.14900997839847574
23	0.13719573992459735
24	0.1331997474996091
25	0.12949331974309822
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1726730.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.24238281317616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86768565025022	36.78655014265185
2	12.933987035912844	12.220647337025046
3	3.9218854159984042	5.558376365160277
4	1.6990926704536284	3.2107674549052834
5	0.8900643210171791	2.1024379690921644
6	0.5642610707919069	1.5994222507754376
7	0.3641423167044406	1.2042065506959612
8	0.25669602944888475	0.9701545671877242
9	0.1892979788483302	0.8048598824262003
>10	1.0831242657941749	9.29792554135141
>50	0.1024909573308335	3.4226114089586313
>100	0.10277811973919977	10.780917941185807
>500	0.01649356016649036	5.427093755445625
>1k	0.007754435003648454	5.857279220112101
>5k	2.461725397983636E-4	0.7567496130264261
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7066	0.4092127894922773	No Hit
TCCATGTACTCTGCGTTGATACCAC	5948	0.3444661296207282	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3310	0.19169181053204612	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3137	0.1816728730027277	No Hit
CATGTACTCTGCGTTGATACCACTG	2789	0.16151917207669989	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2461	0.14252372982458172	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2441	0.14136547115067208	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2437	0.14113381941589015	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2409	0.13951225727241665	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2252	0.13041992668222593	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2141	0.12399159104202742	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2138	0.12381785224094097	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2058	0.1191848175453024	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2056	0.11906899167791142	No Hit
GAACTACGACGGTATCTGATCGTCT	2029	0.1175053424681334	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1945	0.1126406560377129	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1890	0.10945544468446138	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1872	0.10841301187794271	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1862	0.10783388254098787	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1770	0.10250589264100352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1582586739096443E-4
2	0.0	0.0	0.0	0.0	1.1582586739096443E-4
3	0.0	0.0	0.0	0.0	1.1582586739096443E-4
4	0.0	0.0	0.0	0.0	1.1582586739096443E-4
5	0.0	5.7912933695482215E-5	0.0	0.0	1.1582586739096443E-4
6	0.0	5.7912933695482215E-5	0.0	0.0	2.8956466847741103E-4
7	0.0	5.7912933695482215E-5	0.0	0.0	2.8956466847741103E-4
8	0.0	5.7912933695482215E-5	0.0	1.1582586739096443E-4	2.8956466847741103E-4
9	0.0	5.7912933695482215E-5	0.0	1.1582586739096443E-4	2.8956466847741103E-4
10	0.0	5.7912933695482215E-5	0.0	1.1582586739096443E-4	2.8956466847741103E-4
11	0.0	5.7912933695482215E-5	0.0	1.1582586739096443E-4	2.8956466847741103E-4
12	0.0	5.7912933695482215E-5	0.0	1.1582586739096443E-4	4.633034695638577E-4
13	0.0	5.7912933695482215E-5	0.0	1.1582586739096443E-4	4.633034695638577E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	215	0.0	14.583888	16
TCCGATA	220	0.0	13.819744	8
GTTCTAG	105	1.3860699E-9	13.561555	1
GATAACG	220	0.0	13.387488	11
CGATAAC	220	0.0	13.387488	10
AACCGCG	115	3.765308E-10	13.218884	7
CGCGGTC	115	3.765308E-10	13.218501	10
CCGATAA	230	0.0	13.218501	9
CGCATCG	325	0.0	13.155714	13
AATTCCG	240	0.0	13.061323	5
ATCGCCA	325	0.0	12.863738	16
GCATCGC	325	0.0	12.863365	14
ATAACGA	245	0.0	12.797735	12
CGCCAGT	340	0.0	12.576045	18
TAACGAA	250	0.0	12.54178	13
ACGAACG	235	0.0	12.534059	15
CGTCTTA	130	1.9645086E-10	12.4252	15
TTATACA	130	1.9826984E-10	12.420517	4
AGAACCG	185	0.0	12.323215	5
AAGTGCG	55	0.0030660836	12.092277	7
>>END_MODULE