FastQCFastQC Report
Thu 2 Feb 2017
SRR4062445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726730
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32640.18902781558205395No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28250.16360403768973725No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC25900.14999449827129893No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23450.13580582951590578No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23120.13389470270395487No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22080.1278717575996247No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG21130.12237002889855392No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20010.1158837803246599No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC19630.11368308884423158No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA19630.11368308884423158No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18560.10748640493881498No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA18360.10632814626490535No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG18040.10447493238664991No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG708.125751E-418.856975
TAGGACG4500.018.5776064
GTGTACG1355.5119017E-817.9309541
GTCCTAA3750.017.6049371
GATCTAG1002.4780336E-517.6049371
CGTCGTA5150.017.51440210
GTATTAG5600.016.8975941
GTCCTAC5350.016.8645421
ACCGTCG5350.016.859668
TACCGTC5750.016.8346277
GTATAGG1702.4610927E-916.8282471
TCCTACA5500.016.8017922
GGCGTTA6200.016.67726542
ATACCGT5450.016.5503066
TGGTATA3750.016.42651644
GTATTAC1753.614332E-916.347441
CGTTAGG953.347588E-416.21037715
CCGTCGT5750.016.0694169
CTATTCC6300.016.0633454
CGTGATT2750.015.99985228
Produced by FastQC (version 0.11.5)