##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062445_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1726730
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30285626588986	32.0	32.0	32.0	32.0	32.0
2	31.436091919408362	32.0	32.0	32.0	32.0	32.0
3	31.509804080545308	32.0	32.0	32.0	32.0	32.0
4	31.60729529225762	32.0	32.0	32.0	32.0	32.0
5	31.54463523538712	32.0	32.0	32.0	32.0	32.0
6	35.140528629258775	36.0	36.0	36.0	36.0	36.0
7	35.15717338553219	36.0	36.0	36.0	36.0	36.0
8	35.094162955412834	36.0	36.0	36.0	36.0	36.0
9	35.218459747615434	36.0	36.0	36.0	36.0	36.0
10	35.09803501415971	36.0	36.0	36.0	36.0	36.0
11	35.22551875510358	36.0	36.0	36.0	36.0	36.0
12	35.13592339277131	36.0	36.0	36.0	36.0	36.0
13	35.17894749034302	36.0	36.0	36.0	36.0	36.0
14	35.143685463274515	36.0	36.0	36.0	36.0	36.0
15	35.10013493713551	36.0	36.0	36.0	36.0	36.0
16	35.11082798121304	36.0	36.0	36.0	36.0	36.0
17	35.07785525241352	36.0	36.0	36.0	36.0	36.0
18	35.09727114256427	36.0	36.0	36.0	36.0	36.0
19	35.074433756290794	36.0	36.0	36.0	36.0	36.0
20	35.06130547335136	36.0	36.0	36.0	36.0	36.0
21	35.0550954694712	36.0	36.0	36.0	36.0	36.0
22	35.04220752520661	36.0	36.0	36.0	36.0	36.0
23	34.98777400056755	36.0	36.0	36.0	36.0	36.0
24	34.96370770184105	36.0	36.0	36.0	32.0	36.0
25	34.93321422573303	36.0	36.0	36.0	32.0	36.0
26	34.88780353616373	36.0	36.0	36.0	32.0	36.0
27	34.86559797999687	36.0	36.0	36.0	32.0	36.0
28	34.84239921701714	36.0	36.0	36.0	32.0	36.0
29	34.80932456145431	36.0	36.0	36.0	32.0	36.0
30	34.80016505186103	36.0	36.0	36.0	32.0	36.0
31	34.797528275989876	36.0	36.0	36.0	32.0	36.0
32	34.75344263434353	36.0	36.0	36.0	32.0	36.0
33	34.73125155641009	36.0	36.0	36.0	32.0	36.0
34	34.71195786254944	36.0	36.0	36.0	32.0	36.0
35	34.67446039624029	36.0	36.0	36.0	32.0	36.0
36	34.65167455247781	36.0	36.0	36.0	32.0	36.0
37	34.62185402465933	36.0	36.0	36.0	32.0	36.0
38	34.576781546622804	36.0	36.0	36.0	32.0	36.0
39	34.53392192178279	36.0	36.0	36.0	32.0	36.0
40	34.50454095313106	36.0	36.0	36.0	32.0	36.0
41	34.48598738656304	36.0	36.0	36.0	32.0	36.0
42	34.41838677731898	36.0	36.0	36.0	32.0	36.0
43	34.428643737005785	36.0	36.0	36.0	32.0	36.0
44	34.39407087384826	36.0	36.0	36.0	32.0	36.0
45	34.3106391850492	36.0	36.0	36.0	32.0	36.0
46	34.35258088989014	36.0	36.0	36.0	32.0	36.0
47	34.30435157783788	36.0	36.0	36.0	32.0	36.0
48	34.28171283292698	36.0	36.0	36.0	32.0	36.0
49	34.283094635524954	36.0	36.0	36.0	32.0	36.0
50	33.74861790783736	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	9.0
21	33.0
22	140.0
23	416.0
24	1230.0
25	2814.0
26	5866.0
27	11179.0
28	19157.0
29	29079.0
30	40698.0
31	58325.0
32	86325.0
33	135141.0
34	295449.0
35	1040865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.76767758676613	17.462351142208423	11.4275288448172	26.34244242620824
2	16.42591228362801	20.066185737054745	36.03323418214167	27.474667797175584
3	18.764199617888032	23.61015312856348	28.459986830514982	29.16566042303351
4	12.479810045027437	15.09024309024309	35.00262056783796	37.427326296891515
5	14.603441186520186	36.344130234605295	33.14913159555923	15.903296983315284
6	35.8344264431221	34.43611461002765	16.020501237892542	13.708957708957708
7	30.75861933169594	30.04374166415228	20.42561397880038	18.772025025351404
8	28.860579743549795	32.35226836405713	19.154945564706445	19.632206327686628
9	27.218731359274468	13.818663022012705	18.289020286900673	40.67358533181215
10	15.979684142859623	26.355249517874828	31.118414575527154	26.546651763738392
11	38.47538410753273	20.436779345931328	21.43890475059795	19.648931795937987
12	24.63899972780921	23.31777405848048	28.303208955656068	23.74001725805424
13	29.96588928205336	18.700317942005988	25.11295917717304	26.220833598767612
14	23.75519044668246	19.340371685208456	24.343875417697035	32.56056245041205
15	25.759093778413533	26.884863296520013	22.17115588424363	25.18488704082283
16	26.60913981919582	25.120487858553453	23.540391375605914	24.729980946644815
17	24.48582001818466	25.617033352058517	25.126047500188214	24.771099129568608
18	25.886733884278375	23.685579100380487	26.376735216275847	24.050951799065285
19	25.753939527314635	25.254787951793272	24.644848934112456	24.346423586779636
20	25.659367706589915	23.747430113567265	25.256467426870444	25.336734752972383
21	26.92297000689164	24.129250085421578	23.760460523648746	25.187319384038037
22	25.676104544427908	24.285151702929817	24.584851134803937	25.453892617838342
23	24.19498126516595	23.697798729390236	25.148343979660975	26.958876025782843
24	24.847428376179252	25.21957688810642	24.64091085462116	25.292083881093163
25	25.49147811180671	23.942538787187342	24.64670214799071	25.91928095301524
26	24.560527702651832	24.88680917109218	25.437561170536217	25.11510195571977
27	25.98176900847266	24.39906644350883	24.638652250207038	24.98051229781147
28	24.75928489109473	23.891517492601622	25.428642578747112	25.920555037556536
29	24.528096459782365	24.59736032848216	25.57058717923474	25.30395603250074
30	24.960474422752835	24.46520301378907	25.72828409768754	24.84603846577056
31	25.08707209581116	24.462770670573857	24.36599815836871	26.084159075246276
32	24.871809721265052	24.510375102071546	24.46520301378907	26.152612162874334
33	24.467809095805364	23.989448263480682	25.208457604836887	26.334285035877063
34	25.227453047089003	24.47597481945643	25.561610674511936	24.734961458942625
35	26.263341692100095	24.172858524494277	25.15639387744465	24.407405905960978
36	24.734613981340452	25.045548522351496	24.728706862103515	25.491130634204538
37	26.102112084691875	24.52259473108129	24.677396002849317	24.697897181377517
38	24.97946986500495	24.2968501155363	24.889588991909566	25.83409102754918
39	25.51070520579361	23.99576077325349	24.75106125450997	25.74247276644293
40	25.999606192050873	24.377696570975196	24.906383742681253	24.71631349429268
41	24.324011281439482	24.651277269752654	25.823666699483994	25.201044749323863
42	26.44403004523	24.96950884040933	24.723958001540485	23.862503112820185
43	25.194905978352146	23.577281914369934	25.052729726129737	26.17508238114818
44	24.80914792700654	24.411286072518575	25.17608427490111	25.603481725573772
45	24.932618301645306	24.672936706954765	25.014912580426586	25.379532410973344
46	24.587457216820233	24.218378090378927	25.576146820869504	25.618017871931336
47	25.208341778969494	23.785478911005196	25.85928315370672	25.146896156318594
48	25.75781969387223	25.41074748223521	24.076086012289124	24.755346811603435
49	24.385051513554522	24.801792984427212	25.30470890063878	25.508446601379486
50	24.44593216422496	25.710983020497135	24.254587720481904	25.588497094795997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	16.5
2	25.0
3	83.0
4	141.0
5	160.0
6	179.0
7	203.5
8	228.0
9	294.0
10	360.0
11	550.0
12	740.0
13	1377.5
14	2015.0
15	2690.5
16	3366.0
17	4040.5
18	4715.0
19	5068.0
20	5421.0
21	6154.5
22	6888.0
23	7335.0
24	7782.0
25	8652.5
26	9523.0
27	12123.0
28	14723.0
29	16937.5
30	19152.0
31	20789.5
32	22427.0
33	26566.0
34	30705.0
35	35887.5
36	41070.0
37	50347.0
38	59624.0
39	64305.0
40	68986.0
41	75748.0
42	82510.0
43	85347.0
44	88184.0
45	101399.5
46	114615.0
47	126062.5
48	137510.0
49	138311.5
50	139113.0
51	130230.5
52	121348.0
53	119890.5
54	118433.0
55	121941.0
56	125449.0
57	122746.5
58	120044.0
59	111327.5
60	102611.0
61	90049.5
62	77488.0
63	67780.5
64	58073.0
65	49704.0
66	41335.0
67	36108.5
68	30882.0
69	28717.5
70	26553.0
71	20544.5
72	14536.0
73	13328.0
74	12120.0
75	8690.0
76	5260.0
77	4790.5
78	4321.0
79	3740.0
80	3159.0
81	2588.0
82	2017.0
83	1772.5
84	1528.0
85	1249.0
86	970.0
87	683.5
88	397.0
89	257.0
90	117.0
91	74.0
92	31.0
93	26.0
94	21.0
95	16.0
96	11.0
97	11.0
98	11.0
99	8.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014478233423870552
2	0.004285557093465684
3	6.370422706503044E-4
4	2.8956466847741103E-4
5	0.0
6	2.8956466847741103E-4
7	5.7912933695482215E-5
8	5.7912933695482215E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	5.7912933695482215E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1726730.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.629792936832885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41207944757366	39.69987346177143
2	12.827945949411612	12.989524944471881
3	3.7724268835487234	5.7299157595024095
4	1.6143508165383604	3.2693699027497707
5	0.8824079648842054	2.233806627394971
6	0.5131301191121298	1.5587803008179715
7	0.36253378085876703	1.2848507180240565
8	0.23957326055648195	0.9703635660141273
9	0.1855022555431444	0.8452746708718375
>10	0.9906933233962103	9.104390325684724
>50	0.08855140475926973	3.1528063694464827
>100	0.09206943879175837	10.167346538759347
>500	0.012873372778408153	4.533859984212938
>1k	0.005861982247310856	4.459836830277978
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3264	0.18902781558205395	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2825	0.16360403768973725	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2590	0.14999449827129893	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2345	0.13580582951590578	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2312	0.13389470270395487	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2208	0.1278717575996247	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2113	0.12237002889855392	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2001	0.1158837803246599	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1963	0.11368308884423158	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1963	0.11368308884423158	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1856	0.10748640493881498	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1836	0.10632814626490535	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1804	0.10447493238664991	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	5.7912933695482215E-5
8	0.0	0.0	0.0	5.7912933695482215E-5	5.7912933695482215E-5
9	0.0	0.0	0.0	5.7912933695482215E-5	5.7912933695482215E-5
10	0.0	0.0	0.0	5.7912933695482215E-5	5.7912933695482215E-5
11	0.0	0.0	0.0	5.7912933695482215E-5	5.7912933695482215E-5
12	0.0	0.0	0.0	5.7912933695482215E-5	1.7373880108644663E-4
13	0.0	0.0	0.0	5.7912933695482215E-5	1.7373880108644663E-4
14	0.0	0.0	0.0	5.7912933695482215E-5	1.7373880108644663E-4
15	0.0	0.0	0.0	1.1582586739096443E-4	4.053905358683755E-4
16	0.0	0.0	0.0	1.1582586739096443E-4	4.633034695638577E-4
17	0.0	0.0	0.0	1.1582586739096443E-4	5.212164032593399E-4
18	0.0	5.7912933695482215E-5	0.0	1.1582586739096443E-4	5.212164032593399E-4
19	0.0	5.7912933695482215E-5	0.0	1.1582586739096443E-4	5.212164032593399E-4
20	0.0	5.7912933695482215E-5	0.0	1.7373880108644663E-4	5.212164032593399E-4
21	0.0	5.7912933695482215E-5	0.0	2.3165173478192886E-4	5.212164032593399E-4
22	0.0	5.7912933695482215E-5	0.0	8.10781071736751E-4	5.791293369548221E-4
23	0.0	5.7912933695482215E-5	0.0	0.0015057362760825376	5.791293369548221E-4
24	0.0	5.7912933695482215E-5	0.0	0.002953559618469593	5.791293369548221E-4
25	0.0	5.7912933695482215E-5	0.0	0.0037064277565108618	5.791293369548221E-4
26	0.0	5.7912933695482215E-5	0.0	0.00498051229781147	6.949552043457865E-4
27	0.0	5.7912933695482215E-5	0.0	0.007297029645630759	6.949552043457865E-4
28	0.0	5.7912933695482215E-5	0.0	0.011872151407573854	6.949552043457865E-4
29	0.0	5.7912933695482215E-5	0.0	0.021891088936892275	6.949552043457865E-4
30	0.0	1.7373880108644663E-4	0.0	0.04030740185205562	6.949552043457865E-4
31	0.0	1.7373880108644663E-4	0.0	0.08015150023454738	6.949552043457865E-4
32	0.0	1.7373880108644663E-4	0.0	0.12821923520179762	7.528681380412688E-4
33	0.0	1.7373880108644663E-4	0.0	0.17362297521905568	7.528681380412688E-4
34	0.0	1.7373880108644663E-4	0.0	0.22829278462759087	8.686940054322332E-4
35	0.0	1.7373880108644663E-4	0.0	0.28603197952198667	8.686940054322332E-4
36	0.0	1.7373880108644663E-4	0.0	0.3786347605010627	8.686940054322332E-4
37	0.0	1.7373880108644663E-4	0.0	0.5154251098897917	8.686940054322332E-4
38	0.0	1.7373880108644663E-4	0.0	0.6960555500860007	9.266069391277154E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	70	8.125751E-4	18.85697	5
TAGGACG	450	0.0	18.577606	4
GTGTACG	135	5.5119017E-8	17.930954	1
GTCCTAA	375	0.0	17.604937	1
GATCTAG	100	2.4780336E-5	17.604937	1
CGTCGTA	515	0.0	17.514402	10
GTATTAG	560	0.0	16.897594	1
GTCCTAC	535	0.0	16.864542	1
ACCGTCG	535	0.0	16.85966	8
TACCGTC	575	0.0	16.834627	7
GTATAGG	170	2.4610927E-9	16.828247	1
TCCTACA	550	0.0	16.801792	2
GGCGTTA	620	0.0	16.677265	42
ATACCGT	545	0.0	16.550306	6
TGGTATA	375	0.0	16.426516	44
GTATTAC	175	3.614332E-9	16.34744	1
CGTTAGG	95	3.347588E-4	16.210377	15
CCGTCGT	575	0.0	16.069416	9
CTATTCC	630	0.0	16.063345	4
CGTGATT	275	0.0	15.999852	28
>>END_MODULE