FastQCFastQC Report
Thu 2 Feb 2017
SRR4062444_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062444_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273485
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50680.39796306984377516No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35400.27797736133523365No Hit
TATCAACGCAGAGTACTTTTTTTTT34920.2742081767747559No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25180.19772514006839498No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20380.16003329446361755No Hit
GTACATGGAAGCAGTGGTATCAACG16870.13247113236512403No Hit
GGTATCAACGCAGAGTACTTTTTTT16520.12972276862310902No Hit
GATTAAGAGGGACGGCCGGGGGCAT15430.1211635786836908No Hit
GCGCAAGACGGACCAGAGCGAAAGC15260.11982865915185495No Hit
GAATAACGCCGCCGCATCGCCAGTC14980.11762996815824293No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14930.1172373447665265No Hit
GAATAGGACCGCGGTTCTATTTTGT14710.11550980184297419No Hit
GTACATGGGGTGGTATCAACGCAAA13640.10710766126024256No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13500.10600831576343656No Hit
TCCATGTACTCTGCGTTGATACCAC13340.10475192090994398No Hit
GAACTACGACGGTATCTGATCGTCT13140.10318142734307824No Hit
GTCCTATTCCATTATTCCTAGCTGC12980.10192503248958568No Hit
GTATCTGATCGTCTTCGAACCTCCG12970.10184650781124237No Hit
ATCAGATACCGTCGTAGTTCCGACC12780.10035453892271994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGG707.3064366E-613.56306651
GCGTTAT1500.013.2918041
TCTAGAT2450.013.177622
ATTAGCT1301.4551915E-1113.1503913
ATTTGCG2350.012.93785716
TTAGCTC1301.9826984E-1012.4227424
TTAGATT853.953319E-612.2908893
CGCGCTT701.0904737E-412.214437512
GTTATTC1650.012.0877323
TTAGGGT802.8661909E-511.87468054
CGGTAAT802.8835346E-511.8676821
TTGCGCG2500.011.78246218
CGCATCG2750.011.74606113
TAATGCA658.0234755E-411.6919934
GGTTCCG1657.2759576E-1211.5161998
CGTTATT1657.2759576E-1211.5098652
TTAATAT2650.011.4686843
CGCGCTA1251.8264473E-811.40148516
GAACCGC1751.8189894E-1211.4010376
ACGCGCG1501.7644197E-1011.40058913
Produced by FastQC (version 0.11.5)