##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062444_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1273485
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.117520818855347	32.0	32.0	32.0	32.0	32.0
2	30.85835482946403	32.0	32.0	32.0	32.0	32.0
3	30.852981385725	32.0	32.0	32.0	32.0	32.0
4	30.884293101214386	32.0	32.0	32.0	32.0	32.0
5	30.752640195997596	32.0	32.0	32.0	32.0	32.0
6	34.38214662913187	36.0	36.0	36.0	32.0	36.0
7	34.23575150080291	36.0	36.0	36.0	32.0	36.0
8	34.23846531368646	36.0	36.0	36.0	32.0	36.0
9	34.34544655021457	36.0	36.0	36.0	32.0	36.0
10	34.08330683125439	36.0	36.0	36.0	32.0	36.0
11	34.369646285586406	36.0	36.0	36.0	32.0	36.0
12	34.16683431685493	36.0	36.0	36.0	32.0	36.0
13	34.28739011452824	36.0	36.0	36.0	32.0	36.0
14	34.20052375960455	36.0	36.0	36.0	32.0	36.0
15	34.15820131371787	36.0	36.0	36.0	32.0	36.0
16	34.15229704315324	36.0	36.0	36.0	32.0	36.0
17	34.08261424359141	36.0	36.0	36.0	32.0	36.0
18	34.10393212326804	36.0	36.0	36.0	32.0	36.0
19	34.084864760872726	36.0	36.0	36.0	32.0	36.0
20	34.04651095222951	36.0	36.0	36.0	32.0	36.0
21	34.021617058701125	36.0	36.0	36.0	32.0	36.0
22	33.985112506232895	36.0	36.0	36.0	32.0	36.0
23	33.97819369682407	36.0	36.0	36.0	32.0	36.0
24	33.9500473111187	36.0	36.0	36.0	32.0	36.0
25	33.527041150857684	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	49.0
5	112.0
6	208.0
7	53.0
8	172.0
9	246.0
10	156.0
11	34.0
12	84.0
13	86.0
14	365.0
15	408.0
16	612.0
17	798.0
18	1085.0
19	1571.0
20	2207.0
21	3037.0
22	4283.0
23	6136.0
24	8155.0
25	10978.0
26	14821.0
27	18658.0
28	25087.0
29	32989.0
30	41659.0
31	57373.0
32	82598.0
33	121336.0
34	280953.0
35	557167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.86776275387198	17.29643515122768	11.001682139945876	25.83411995495446
2	17.436119729519405	20.26177973468652	35.769182142500966	26.53291839329311
3	19.134273107135314	24.25156683500507	28.05366935050066	28.56049070735896
4	13.142344640400093	15.216711798596409	34.827896558873604	36.81304700212989
5	15.286528641081373	36.081486258580775	32.32766374271532	16.304321357622527
6	33.974405304339626	34.746488388901106	16.921330484240958	14.357775822518304
7	30.32052652091374	30.39415207251606	20.646427628518627	18.63889377805157
8	29.101476384528024	30.91612862661265	19.61677663249146	20.365618356367865
9	28.210063822429028	13.818734869682775	18.717263495456976	39.253937812431225
10	17.121397108016595	25.745146026058197	29.97290092209192	27.160555943833288
11	37.72021955526066	21.08125148385332	21.675893417272917	19.522635543613102
12	25.320615890722877	23.639755977321208	27.81124174332016	23.22838638863576
13	29.787126988731732	19.112343835865214	25.176359043663453	25.9241701317396
14	24.343020760119437	19.37823418697787	24.49506980576403	31.783675247138664
15	25.970594639806745	26.176241921701525	22.33458403985281	25.518579398638924
16	26.201167207506355	25.35437577038211	23.249110130053076	25.195346892058463
17	24.247658341994494	25.090136340551055	24.952868088826552	25.7093372286279
18	25.294377576983194	24.009856616733604	26.10438767262446	24.591378133658743
19	25.943290895595617	24.45322623256048	25.36545937173697	24.238023500106934
20	26.16902225657048	23.38296097532979	24.841285627691263	25.606731140408463
21	27.63938772557727	23.25922594523874	24.17175637340311	24.92962995578088
22	26.542157939810206	23.601636034919483	24.544141250744133	25.31206477452617
23	24.925574526908758	23.50924951759083	25.289484816888958	26.275691138611446
24	24.90509663317136	24.79572581025617	24.84974288814455	25.449434668427923
25	25.117329364302464	23.909322426361033	25.114813338396836	25.858534870939664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	367.0
1	367.0
2	786.5
3	1206.0
4	1206.0
5	1206.0
6	3054.0
7	4902.0
8	4902.0
9	4902.0
10	4827.5
11	4753.0
12	4753.0
13	4753.0
14	4298.5
15	3844.0
16	3844.0
17	3844.0
18	6195.5
19	8547.0
20	8547.0
21	8547.0
22	13585.5
23	18624.0
24	18624.0
25	18624.0
26	27541.5
27	36459.0
28	36459.0
29	36459.0
30	43760.0
31	51061.0
32	51061.0
33	51061.0
34	61466.0
35	71871.0
36	71871.0
37	71871.0
38	81155.5
39	90440.0
40	90440.0
41	90440.0
42	105046.0
43	119652.0
44	119652.0
45	119652.0
46	140348.5
47	161045.0
48	161045.0
49	161045.0
50	163956.0
51	166867.0
52	166867.0
53	166867.0
54	154659.5
55	142452.0
56	142452.0
57	142452.0
58	133242.0
59	124032.0
60	124032.0
61	124032.0
62	113222.5
63	102413.0
64	102413.0
65	102413.0
66	87356.0
67	72299.0
68	72299.0
69	72299.0
70	56027.5
71	39756.0
72	39756.0
73	39756.0
74	32322.0
75	24888.0
76	24888.0
77	24888.0
78	20381.0
79	15874.0
80	15874.0
81	15874.0
82	11430.5
83	6987.0
84	6987.0
85	6987.0
86	5191.0
87	3395.0
88	3395.0
89	3395.0
90	2232.0
91	1069.0
92	1069.0
93	1069.0
94	620.0
95	171.0
96	171.0
97	171.0
98	341.0
99	511.0
100	511.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008402140582731638
2	0.0037691845604777437
3	0.004554431343910608
4	0.014212966780134827
5	0.03423675975767285
6	0.04436644326395678
7	0.06509695834658437
8	0.08794763974448071
9	0.09462223740366003
10	0.11668767201812351
11	0.11456750570285476
12	0.12846637376961645
13	0.11825816558498922
14	0.11990718383019824
15	0.11024864839397402
16	0.10907077821882472
17	0.11959308511682509
18	0.12948719458807917
19	0.1304294907281986
20	0.12139915271872069
21	0.12854489844795972
22	0.1471552472153186
23	0.13686851435234806
24	0.13074358944157174
25	0.12862342312630304
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1273485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.222306300503426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.045914894796	35.15017571169277
2	13.973840061856116	12.918062310667183
3	4.394580298778507	6.093829098968939
4	1.8185243321700764	3.3622555478593483
5	0.9467584858269759	2.1880680362247653
6	0.5714691901958665	1.5848774370320395
7	0.36273482247542876	1.17365080492226
8	0.24832896870896826	0.918267012396325
9	0.19319975605778814	0.8037124471517063
>10	1.1514285531211041	10.301591460784817
>50	0.13903560130439802	4.523159508072803
>100	0.13489056496355745	12.853946446823192
>500	0.014001296629325618	4.1932024540539095
>1k	0.005122425596094738	3.535217721145055
>5k	1.707475198698246E-4	0.3999840022048789
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5068	0.39796306984377516	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3540	0.27797736133523365	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3492	0.2742081767747559	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2518	0.19772514006839498	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2038	0.16003329446361755	No Hit
GTACATGGAAGCAGTGGTATCAACG	1687	0.13247113236512403	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1652	0.12972276862310902	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1543	0.1211635786836908	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1526	0.11982865915185495	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1498	0.11762996815824293	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1493	0.1172373447665265	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1471	0.11550980184297419	No Hit
GTACATGGGGTGGTATCAACGCAAA	1364	0.10710766126024256	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1350	0.10600831576343656	No Hit
TCCATGTACTCTGCGTTGATACCAC	1334	0.10475192090994398	No Hit
GAACTACGACGGTATCTGATCGTCT	1314	0.10318142734307824	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1298	0.10192503248958568	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1297	0.10184650781124237	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1278	0.10035453892271994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.35574035029859E-4
2	0.0	0.0	0.0	0.0	2.35574035029859E-4
3	0.0	0.0	0.0	0.0	2.35574035029859E-4
4	0.0	0.0	0.0	0.0	2.35574035029859E-4
5	0.0	0.0	0.0	0.0	2.35574035029859E-4
6	0.0	0.0	0.0	0.0	2.35574035029859E-4
7	0.0	0.0	0.0	0.0	2.35574035029859E-4
8	0.0	0.0	0.0	0.0	2.35574035029859E-4
9	0.0	0.0	0.0	0.0	2.35574035029859E-4
10	0.0	0.0	0.0	0.0	2.35574035029859E-4
11	0.0	0.0	0.0	0.0	2.35574035029859E-4
12	0.0	0.0	0.0	0.0	7.06722105089577E-4
13	0.0	0.0	0.0	0.0	7.06722105089577E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTAGG	70	7.3064366E-6	13.5630665	1
GCGTTAT	150	0.0	13.291804	1
TCTAGAT	245	0.0	13.17762	2
ATTAGCT	130	1.4551915E-11	13.150391	3
ATTTGCG	235	0.0	12.937857	16
TTAGCTC	130	1.9826984E-10	12.422742	4
TTAGATT	85	3.953319E-6	12.290889	3
CGCGCTT	70	1.0904737E-4	12.2144375	12
GTTATTC	165	0.0	12.087732	3
TTAGGGT	80	2.8661909E-5	11.8746805	4
CGGTAAT	80	2.8835346E-5	11.867682	1
TTGCGCG	250	0.0	11.782462	18
CGCATCG	275	0.0	11.746061	13
TAATGCA	65	8.0234755E-4	11.691993	4
GGTTCCG	165	7.2759576E-12	11.516199	8
CGTTATT	165	7.2759576E-12	11.509865	2
TTAATAT	265	0.0	11.468684	3
CGCGCTA	125	1.8264473E-8	11.401485	16
GAACCGC	175	1.8189894E-12	11.401037	6
ACGCGCG	150	1.7644197E-10	11.400589	13
>>END_MODULE