FastQCFastQC Report
Thu 2 Feb 2017
SRR4062444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273485
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16380.12862342312630304No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14470.11362520956273532No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13800.10836405611373515No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13710.10765733400864556No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13610.10687208722521271No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13010.10216060652461553No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12960.1017679831328991No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12960.1017679831328991No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC12900.10129683506283936No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG12830.10074716231443637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGC701.1323937E-625.14274632
CGATTAG701.1323937E-625.14274614
TATGCCG551.5937313E-423.9998955
TAGGACG2100.020.9522884
CTACTAG1601.8189894E-1220.6281471
TATATGT1601.1023076E-917.874924
ACCGTTC1002.4832769E-517.5999228
GTTATAT1154.2670363E-617.2200181
CGCGGCA1154.273428E-617.21731626
GCATTCG6400.016.49992622
CCGTTCG800.001988488716.4999269
CATATAG953.345601E-416.2110922
ATACCGT4550.015.9559736
CGTATTG6500.015.90762127
TACGCTA4050.015.75301559
CGCGGTT5200.015.65377710
TTCGTAT6750.015.64437625
ACGCTAT4100.015.56090510
CCTTATA850.00297936715.5299522
TAAACGC4250.015.52934328
Produced by FastQC (version 0.11.5)