##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062444_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1273485
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189119620568754	32.0	32.0	32.0	32.0	32.0
2	31.437453130582615	32.0	32.0	32.0	32.0	32.0
3	31.512254953925645	32.0	32.0	32.0	32.0	32.0
4	31.609338939995368	32.0	32.0	32.0	32.0	32.0
5	31.54960992866033	32.0	32.0	32.0	32.0	32.0
6	35.135352988060326	36.0	36.0	36.0	36.0	36.0
7	35.14813837618818	36.0	36.0	36.0	36.0	36.0
8	35.101572456683826	36.0	36.0	36.0	36.0	36.0
9	35.20200709077845	36.0	36.0	36.0	36.0	36.0
10	35.090906449624455	36.0	36.0	36.0	36.0	36.0
11	35.208205828886875	36.0	36.0	36.0	36.0	36.0
12	35.11242221149052	36.0	36.0	36.0	36.0	36.0
13	35.15591781607164	36.0	36.0	36.0	36.0	36.0
14	35.10805702462141	36.0	36.0	36.0	36.0	36.0
15	35.086683392423154	36.0	36.0	36.0	36.0	36.0
16	35.09914839986337	36.0	36.0	36.0	36.0	36.0
17	35.07147316222806	36.0	36.0	36.0	36.0	36.0
18	35.083767771116264	36.0	36.0	36.0	36.0	36.0
19	35.052459196614016	36.0	36.0	36.0	36.0	36.0
20	35.02725670109974	36.0	36.0	36.0	36.0	36.0
21	35.02590529138545	36.0	36.0	36.0	36.0	36.0
22	35.01831352548322	36.0	36.0	36.0	36.0	36.0
23	34.985297039227	36.0	36.0	36.0	36.0	36.0
24	34.96437256818887	36.0	36.0	36.0	32.0	36.0
25	34.93639344004837	36.0	36.0	36.0	32.0	36.0
26	34.90293957133378	36.0	36.0	36.0	32.0	36.0
27	34.88381253018292	36.0	36.0	36.0	32.0	36.0
28	34.87787999073409	36.0	36.0	36.0	32.0	36.0
29	34.845210583556145	36.0	36.0	36.0	32.0	36.0
30	34.82215102651386	36.0	36.0	36.0	32.0	36.0
31	34.83500080487795	36.0	36.0	36.0	32.0	36.0
32	34.783837265456604	36.0	36.0	36.0	32.0	36.0
33	34.746107728006216	36.0	36.0	36.0	32.0	36.0
34	34.717780735540664	36.0	36.0	36.0	32.0	36.0
35	34.69451937007503	36.0	36.0	36.0	32.0	36.0
36	34.66471140217592	36.0	36.0	36.0	32.0	36.0
37	34.6487913088886	36.0	36.0	36.0	32.0	36.0
38	34.60643980887093	36.0	36.0	36.0	32.0	36.0
39	34.601626246088486	36.0	36.0	36.0	32.0	36.0
40	34.5839841066051	36.0	36.0	36.0	32.0	36.0
41	34.532784445831716	36.0	36.0	36.0	32.0	36.0
42	34.50108638892488	36.0	36.0	36.0	32.0	36.0
43	34.47970961573949	36.0	36.0	36.0	32.0	36.0
44	34.4384205546198	36.0	36.0	36.0	32.0	36.0
45	34.38166841384076	36.0	36.0	36.0	32.0	36.0
46	34.36893171101348	36.0	36.0	36.0	32.0	36.0
47	34.31555377566285	36.0	36.0	36.0	32.0	36.0
48	34.26644836806087	36.0	36.0	36.0	32.0	36.0
49	34.24136130382376	36.0	36.0	36.0	32.0	36.0
50	33.708554085835324	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	6.0
21	31.0
22	120.0
23	345.0
24	965.0
25	2178.0
26	4617.0
27	8609.0
28	14365.0
29	21299.0
30	30337.0
31	41895.0
32	61983.0
33	98183.0
34	213532.0
35	775019.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04747973966878	18.12815665738109	11.530580750348896	26.293782852601232
2	16.843106290181865	20.710695295929337	35.522907048606555	26.923291365282243
3	18.70223223154838	24.473823282519774	28.42567486389646	28.398269622035382
4	12.850593883541345	15.590797514216925	35.000023557458995	36.55858504478273
5	14.90743903540285	36.319312752015136	32.39315735952917	16.38009085305284
6	34.24911934089164	34.90315498186855	16.66397063789088	14.183755039348933
7	30.287675159110627	30.372638861078066	20.639033832357665	18.700652147453642
8	29.096455788642977	30.994318739521866	19.668704382069674	20.240521089765487
9	28.39295319536547	13.551082266379266	18.70795494253957	39.348009595715695
10	17.121599390648495	25.740860708999318	30.061759659516994	27.07578024083519
11	38.0226700746377	20.82191780821918	21.729898663902596	19.425513453240516
12	25.322795321499665	23.617474881918515	27.779125784756005	23.28060401182582
13	30.008441402921903	18.92421190669698	25.248353926430227	25.81899276395089
14	24.396910839153975	19.217266006274123	24.58246465407916	31.80335850049274
15	26.18829432619937	26.160261016030812	22.16335488835754	25.48808976941228
16	26.398818988837718	25.13998987031649	23.220375583536516	25.240815557309272
17	24.41591381131305	24.982233791524834	24.934333737735425	25.66751865942669
18	25.534969002383225	23.806483782690805	26.162381182346085	24.49616603257989
19	26.208004020463534	24.264596756145536	25.316199248518828	24.211199974872102
20	26.439651821576227	23.125439247419482	24.866881039038542	25.568027891965748
21	27.94096514682152	23.123790229174272	24.037110762985037	24.898133861019172
22	26.619865958374067	23.525915106970245	24.543202314907518	25.311016619748173
23	25.02667875946713	23.332430299532387	25.201945841529344	26.438945099471134
24	25.15757939826539	24.500563414567115	24.891066639968276	25.45079054719922
25	25.395038025575488	23.708092360726667	25.032489585664536	25.864380028033313
26	24.95467162942634	24.433503339261947	25.54721885220478	25.064606179106942
27	26.089510280843513	24.204839475926295	24.565817422270385	25.139832820959807
28	24.881015481140338	24.017479593399216	25.53512605173991	25.56637887372054
29	24.833508050742648	24.330007813205494	25.337872059741574	25.498612076310284
30	24.78623619437999	24.419525946516842	25.696729839770395	25.097508019332775
31	25.219927992869962	24.37908573717005	24.448030404755457	25.952955865204537
32	25.017255798065936	24.320820425839333	24.57720350063016	26.084720275464573
33	24.88502023973584	23.891918632728302	25.114547874533265	26.108513253002585
34	25.557584109746088	23.851321374024824	25.475290246842324	25.11580426938676
35	26.223866005488876	23.899142903135882	25.00846103409149	24.868530057283753
36	24.937788823582533	24.56746644051559	24.665229665052983	25.829515070848892
37	26.00996478168176	24.468368296446368	24.472451579720218	25.049215342151655
38	24.927423566041217	24.11602806472004	25.262095745140307	25.69445262409844
39	25.441132011762996	23.9287467068713	24.771552079529794	25.858569201835905
40	25.75585892256289	24.20853013580843	25.206264698838226	24.829346242790454
41	24.425336772714246	24.7492510708803	25.66382799954456	25.1615841568609
42	25.96065128368218	24.698210029957167	25.054240921565622	24.286897764795032
43	24.94320702638822	23.674640847752425	25.201160594745915	26.180991531113442
44	24.453998280309545	24.27731775403715	25.21780782655469	26.050876139098616
45	24.704806102938	24.41897627376844	25.20202436620769	25.67419325708587
46	24.58387809828934	24.20821603709506	25.309917274251365	25.897988590364236
47	24.48941291024237	24.101108375834816	25.736384802333756	25.673093911589064
48	25.25408622794929	24.91792207996168	24.65188046973463	25.176111222354407
49	24.10770444881565	24.930093405104888	25.248510975786914	25.713691170292545
50	24.053443896080438	25.308582354719526	24.82565558290832	25.81231816629171
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	40.0
2	38.0
3	109.0
4	180.0
5	228.0
6	276.0
7	287.5
8	299.0
9	407.0
10	515.0
11	719.5
12	924.0
13	1652.0
14	2380.0
15	3386.5
16	4393.0
17	5334.5
18	6276.0
19	6811.5
20	7347.0
21	7857.0
22	8367.0
23	8594.0
24	8821.0
25	8940.5
26	9060.0
27	10250.5
28	11441.0
29	13138.5
30	14836.0
31	16172.0
32	17508.0
33	19977.5
34	22447.0
35	26045.0
36	29643.0
37	35682.0
38	41721.0
39	45727.5
40	49734.0
41	54462.0
42	59190.0
43	59850.5
44	60511.0
45	67280.5
46	74050.0
47	79607.0
48	85164.0
49	89693.5
50	94223.0
51	91003.5
52	87784.0
53	86590.5
54	85397.0
55	88176.0
56	90955.0
57	90177.0
58	89399.0
59	81994.0
60	74589.0
61	66615.0
62	58641.0
63	52521.0
64	46401.0
65	40611.5
66	34822.0
67	31261.5
68	27701.0
69	25479.5
70	23258.0
71	19000.0
72	14742.0
73	13113.5
74	11485.0
75	8698.5
76	5912.0
77	5292.0
78	4672.0
79	4019.0
80	3366.0
81	2704.5
82	2043.0
83	1741.0
84	1439.0
85	1178.5
86	918.0
87	657.0
88	396.0
89	258.5
90	121.0
91	77.0
92	33.0
93	23.0
94	13.0
95	8.5
96	4.0
97	4.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014448540815164687
2	0.004397381987224035
3	4.7114807005971796E-4
4	2.3557403502985898E-4
5	0.0
6	5.496727484030044E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1273485.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.83934878194055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26193523730736	39.27946473097172
2	13.517638617166677	13.744558887327308
3	4.146658934332376	6.324403195268208
4	1.6933907043797096	3.443635225762242
5	0.9016312992920883	2.2919174048712354
6	0.4994108434639194	1.523383323380719
7	0.33798006975235856	1.202788065323933
8	0.23186588827487867	0.9430328611712814
9	0.179803443726392	0.8226980989020057
>10	0.9940180750536438	9.703240656460567
>50	0.1146751718771298	4.105679221838251
>100	0.10760010312452911	11.25840491513738
>500	0.010900149504894839	3.745385469661676
>1k	0.002491462743975963	1.6114079439234725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1638	0.12862342312630304	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1447	0.11362520956273532	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1380	0.10836405611373515	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1371	0.10765733400864556	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1361	0.10687208722521271	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1301	0.10216060652461553	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1296	0.1017679831328991	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1296	0.1017679831328991	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1290	0.10129683506283936	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1283	0.10074716231443637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.852467834328634E-5	0.0
10	7.852467834328634E-5	0.0	0.0	7.852467834328634E-5	0.0
11	7.852467834328634E-5	0.0	0.0	7.852467834328634E-5	0.0
12	7.852467834328634E-5	0.0	0.0	7.852467834328634E-5	7.852467834328634E-5
13	7.852467834328634E-5	0.0	0.0	7.852467834328634E-5	7.852467834328634E-5
14	7.852467834328634E-5	0.0	0.0	7.852467834328634E-5	7.852467834328634E-5
15	7.852467834328634E-5	0.0	0.0	7.852467834328634E-5	1.5704935668657267E-4
16	7.852467834328634E-5	0.0	0.0	1.5704935668657267E-4	1.5704935668657267E-4
17	7.852467834328634E-5	0.0	0.0	1.5704935668657267E-4	1.5704935668657267E-4
18	7.852467834328634E-5	0.0	0.0	2.35574035029859E-4	2.35574035029859E-4
19	7.852467834328634E-5	0.0	0.0	3.1409871337314535E-4	2.35574035029859E-4
20	7.852467834328634E-5	0.0	0.0	3.1409871337314535E-4	2.35574035029859E-4
21	7.852467834328634E-5	0.0	0.0	3.1409871337314535E-4	3.1409871337314535E-4
22	7.852467834328634E-5	0.0	0.0	0.0012563948534925814	3.1409871337314535E-4
23	7.852467834328634E-5	0.0	0.0	0.0027483637420150217	3.1409871337314535E-4
24	7.852467834328634E-5	0.0	0.0	0.004632956022253894	3.1409871337314535E-4
25	7.852467834328634E-5	0.0	0.0	0.005653776840716616	3.1409871337314535E-4
26	7.852467834328634E-5	0.0	0.0	0.0072242704075823425	3.926233917164317E-4
27	7.852467834328634E-5	0.0	0.0	0.010600831576343655	5.496727484030044E-4
28	7.852467834328634E-5	0.0	0.0	0.02120166315268731	5.496727484030044E-4
29	7.852467834328634E-5	0.0	0.0	0.0448375913340165	5.496727484030044E-4
30	7.852467834328634E-5	0.0	0.0	0.08983223202471957	5.496727484030044E-4
31	7.852467834328634E-5	0.0	0.0	0.18688873445702148	5.496727484030044E-4
32	7.852467834328634E-5	0.0	0.0	0.2967447594592791	5.496727484030044E-4
33	7.852467834328634E-5	0.0	0.0	0.4113122651621338	5.496727484030044E-4
34	7.852467834328634E-5	0.0	0.0	0.5393860155400338	5.496727484030044E-4
35	7.852467834328634E-5	0.0	0.0	0.6827720781948747	5.496727484030044E-4
36	7.852467834328634E-5	0.0	0.0	0.8896846056294342	5.496727484030044E-4
37	7.852467834328634E-5	0.0	0.0	1.1788909959677578	5.496727484030044E-4
38	7.852467834328634E-5	0.0	0.0	1.5562020754072485	7.852467834328634E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGC	70	1.1323937E-6	25.142746	32
CGATTAG	70	1.1323937E-6	25.142746	14
TATGCCG	55	1.5937313E-4	23.999895	5
TAGGACG	210	0.0	20.952288	4
CTACTAG	160	1.8189894E-12	20.628147	1
TATATGT	160	1.1023076E-9	17.87492	4
ACCGTTC	100	2.4832769E-5	17.599922	8
GTTATAT	115	4.2670363E-6	17.220018	1
CGCGGCA	115	4.273428E-6	17.217316	26
GCATTCG	640	0.0	16.499926	22
CCGTTCG	80	0.0019884887	16.499926	9
CATATAG	95	3.345601E-4	16.211092	2
ATACCGT	455	0.0	15.955973	6
CGTATTG	650	0.0	15.907621	27
TACGCTA	405	0.0	15.7530155	9
CGCGGTT	520	0.0	15.653777	10
TTCGTAT	675	0.0	15.644376	25
ACGCTAT	410	0.0	15.560905	10
CCTTATA	85	0.002979367	15.529952	2
TAAACGC	425	0.0	15.529343	28
>>END_MODULE