FastQCFastQC Report
Thu 2 Feb 2017
SRR4062443_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062443_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073633
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG49240.45862971797625446No Hit
GTATCAACGCAGAGTACTTTTTTTT43970.409544043448739No Hit
TCCATGTACTCTGCGTTGATACCAC40580.3779690080316086No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32710.3046664921812202No Hit
TATCAACGCAGAGTACTTTTTTTTT32250.30038197410101963No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22370.2083579770741026No Hit
GAGTACATGGAAGCAGTGGTATCAA20960.19522499774131383No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17870.16644421324605335No Hit
CATGTACTCTGCGTTGATACCACTG17730.16514022948251403No Hit
GTACATGGGGTGGTATCAACGCAAA15450.14390392247630243No Hit
GGTATCAACGCAGAGTACTTTTTTT15380.14325193059453276No Hit
GCTTCCATGTACTCTGCGTTGATAC13290.12378531583883878No Hit
CCCATGTACTCTGCGTTGATACCAC12200.11363287082271131No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11870.11055919480865435No Hit
GTACATGGGAAGCAGTGGTATCAAC11620.10823065237376274No Hit
GCGTTGATACCACTGCTTCCATGTA11520.10729923539980607No Hit
GAATAGGACCGCGGTTCTATTTTGT11250.10478440957012312No Hit
GTCTTGCGCCGGTCCAAGAATTTCA10970.1021764420430445No Hit
CATGGAAGCAGTGGTATCAACGCAG10910.10161759185867052No Hit
GCGCAAGACGGACCAGAGCGAAAGC10870.10124502506908785No Hit
GATTAAGAGGGACGGCCGGGGGCAT10840.10096559997690085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCG400.005271250414.25262412
CGTGTCG400.005274512314.2512949
AAGGGCG400.00527614414.250635
TCGCGTA1101.8553692E-1013.8194379
AACCGCG707.2542207E-613.5720277
GCGTAAC1153.765308E-1013.21797611
CGCGTAA1207.366907E-1012.66840810
GTCGCGT1207.385097E-1012.6672278
TACAGTC751.4769012E-512.6672267
CTTATAC604.106388E-412.6607333
GTGTATA2050.012.5051691
CCGTCGT2250.012.2455569
TAGGAGT701.0922179E-412.2119784
CGTCGTA2300.011.97990610
GGGTCGC1353.7107384E-1011.9634916
TATTAGC1201.0073563E-811.8688852
ACCGTCG2250.011.8227448
ATTAGCT1052.7332135E-711.7563953
CGCCAGT1950.011.69391418
CGAACGT658.032586E-411.69014
Produced by FastQC (version 0.11.5)